A Random Walk Towards the Golden Fleece: Single-molecule Investigations of Argonaute Target Search

In this thesis we used single-molecule FRET to investigate the kinetic properties of a protein called Argonaute. While traditionally one uses bulk methods to investigate the molecular properties of proteins, bulk methods do not confer information that is transient, since that is inherently averaged out. Single-molecule methods, as their name...

Numerical simulation on the mechanical characteristics of double-stranded DNA under axial stretching and lateral unzipping

The mechanical characteristics of the long-chain double-stranded DNA (dsDNA) molecule under the axial stretching and lateral unzipping are studied by the clustered atomistic-continuum method (CACM). The CACM consisted of the clustered atom method (CAM) and the atomistic-continuum method (ACM). The CAM treats the specific atomic group as the...