Precision Engineering of the Co-immobilization of Enzymes for Cascade Biocatalysis

The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...

Risk Quantification and Visualization Method for Loss-of-Control Scenarios in Flight

This paper proposes a flight risk analysis method that combines risk assessment and visual deduction to study the causes of flight accidents, specifically the loss of control caused by failure factors. The goal is to explore the impact of these failure factors on loss-of-control events and illustrate the risk evolution under different scenarios...

Designing an enzyme assembly line for green cascade processes using bio-orthogonal chemistry

Two non-canonical amino acids (ncAAs) with bio-orthogonal reactive groups, namely, p-azido-l-phenylalanine (p-AzF) and p-propargyloxy-l-phenylalanine (p-PaF), were genetically inserted into an aldo-keto reductase (AKR) and an alcohol dehydrogenase (ADH), respectively, at two preselected sites for each enzyme. The variants were expressed in...

Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry

The covalent immobilisation of enzymes generally involves the use of highly reactive crosslinkers, such as glutaraldehyde, to couple enzyme molecules to each other or to carriers through, for example, the free amino groups of lysine residues, on the enzyme surface. Unfortunately, such methods suffer from a lack of precision. Random formation...

A Coflow-based Co-optimization Framework for High-performance Data Analytics

Efficient execution of distributed database operators such as joining and aggregating is critical for the performance of big data analytics. With the increase of the compute speedup of modern CPUs, reducing the network<br/>communication time of these operators in large systems is becoming increasingly important, and also challenging current...