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Mateo, C. (author), Van Langen, L.M. (author), Van Rantwijk, F. (author)The invention relates to a method of preparing cross-linked enzyme particles using a cross-linking agent. According to the invention, the enzyme particles are formed and subsequently cross-linked using a cross-linking agent having at least n reactive groups where N>=3 and a molecular weight of >2,000 Da. The method according to the invention...patent 2004