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Almeida Benalcazar, E.F. (author), Mondini, Camille (author), Bruant, Guillaume (author), Tremblay, Julien (author), Weissbrodt, D.G. (author), Mockaitis, Gustavo (author)BACKGROUND: Carboxylates such as volatile fatty acids (VFA) can be produced by acidogenic fermentation (AF) of dairy wastes including cheese whey, a massive residue produced at 160.67 million m<sup>3</sup> of which 42% are not valorized and impact the environment. In mixed-culture fermentations, selection pressures can favor AF and halt...journal article 2024
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Martinez Quintela, M. (author), Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Suárez, S. (author), Omil, F. (author), Weissbrodt, D.G. (author)In activated sludge, the antibiotic resistance genes (ARGs) can be present either in the intracellular (iDNA) or extracellular DNA fraction (exDNA). Recent advances in the exDNA extraction methodology allow a better profiling of the pool of ARGs. However, little is known about how stress conditions modify the distribution of ARGs between both...journal article 2024
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Policastro, G. (author), Ebrahimi, S. (author), Weissbrodt, D.G. (author), Fabbricino, Massimiliano (author), van Loosdrecht, Mark C.M. (author)A dual-growth-limited continuous operated bioreactor (chemostat) was used to enhance lipid accumulation in an enrichment culture of microalgae. The light intensity and nitrogen concentration where both limiting factors resulting in high lipid accumulation in the mixed culture. Both conditions of light and nitrogen excess and deficiency were...journal article 2024
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Calderon Franco, D. (author), Corbera Rubio, F. (author), Cuesta Sanz, M. (author), Pieterse, Brent (author), de Ridder, David (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author), Laureni, M. (author), Weissbrodt, D.G. (author)Drinking water treatment plants (DWTPs) are designed to remove physical, chemical, and biological contaminants. However, until recently, the role of DWTPs in minimizing the cycling of antibiotic resistance determinants has got limited attention. In particular, the risk of selecting antibiotic-resistant bacteria (ARB) is largely overlooked in...journal article 2023
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Teixeira, A. Margarida (author), Vaz-Moreira, Ivone (author), Calderon Franco, D. (author), Weissbrodt, D.G. (author), Purkrtova, Sabina (author), Gajdos, Stanislav (author), Dottorini, Giulia (author), Nielsen, Per H. (author), Khalifa, Leron (author), Cytryn, Eddie (author), Bartacek, Jan (author), Manaia, Célia M. (author)Urban wastewater treatment plants (UWTPs) are essential for reducing the pollutants load and protecting water bodies. However, wastewater catchment areas and UWTPs emit continuously antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), with recognized impacts on the downstream environments. Recently, the European Commission...journal article 2023
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Alloul, A. (author), Moradvandi, A. (author), Puyol, Daniel (author), Molina, Raúl (author), Gardella, G. (author), Vlaeminck, Siegfried E. (author), De Schutter, B.H.K. (author), Abraham, E. (author), Lindeboom, R.E.F. (author), Weissbrodt, D.G. (author)Purple phototrophic bacteria (PPB) show an underexplored potential for resource recovery from wastewater. Raceway reactors offer a more affordable full-scale solution on wastewater and enable useful additional aerobic processes. Current mathematical models of PPB systems provide useful mechanistic insights, but do not represent the full...journal article 2023
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Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)The transformation of environmental microorganisms by extracellular DNA is an overlooked mechanism of horizontal gene transfer and evolution. It initiates the acquisition of exogenous genes and propagates antimicrobial resistance alongside vertical and conjugative transfers. We combined mixed-culture biotechnology and Hi-C sequencing to...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Giulianetti de Almeida, M.P. (author), Mockaitis, Gustavo (author), Weissbrodt, D.G. (author)Whey has applications in food, beverages, personal care products, pharmaceuticals, and the medical sector. However, it remains a massive dairy residue worldwide (160.7 million m<sup>3</sup> year<sup>−1</sup>), with high organic and nutrient loads. About 42% is used for low-value products such as animal feed and fertilizers or is even directly...review 2023
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Cerruti, M. (author), Crosset-Perrotin, G. (author), Ananth, Mythili (author), Rombouts, Julius Laurens (author), Weissbrodt, D.G. (author)Fermentative chemoorganoheterotrophic bacteria (FCB) and purple photoorganoheterotrophic bacteria (PPB) are two interesting microbial guilds to process carbohydrate-rich wastewaters. Their metabolic interactions have been studied in pure cultures or co-cultures, but little is known about mixed cultures. We studied the effect of reactor...journal article 2023
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Piaggio, A.L. (author), Mittapalli, Srilekha (author), Calderon Franco, D. (author), Weissbrodt, D.G. (author), van Lier, J.B. (author), de Kreuk, M.K. (author), Lindeboom, R.E.F. (author)This study investigates the effects, conversions, and resistance induction, following the addition of 150 μg·L<sup>−1 </sup>of two antibiotics, sulfamethoxazole (SMX) and trimethoprim (TMP), in a laboratory-scale micro-aerated anaerobic membrane bioreactor (MA-AnMBR). TMP and SMX were removed at 97 and 86%, indicating that micro-aeration did not...journal article 2023
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Calderon Franco, D. (author), Sarelse, R.G. (author), Christou, S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)In the One Health context, wastewater treatment plants (WWTPs) are central to safeguarding water resources. Nonetheless, many questions remain about their effectiveness in preventing antimicrobial resistance (AMR) dissemination. Most surveillance studies monitor the levels and removal of selected antibiotic resistance genes (ARGs) and mobile...journal article 2022
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Miłobedzka, Aleksandra (author), Ferreira, Catarina (author), Vaz-Moreira, Ivone (author), Calderon Franco, D. (author), Gorecki, Adrian (author), Purkrtova, Sabina (author), Bartacek, Jan (author), Dziewit, Lukasz (author), Weissbrodt, D.G. (author)Antibiotic resistance (AR) is a global problem requiring international cooperation and coordinated action. Global monitoring must rely on methods available and comparable across nations to quantify AR occurrence and identify sources and reservoirs, as well as paths of AR dissemination. Numerous analytical tools that are gaining relevance in...review 2022
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Seviour, R (author), Tooker, Nick (author), Vainio, Pirjo (author), Dueholm, Morten Kam Dahl (author), Nierychlo, Marta (author), Andersen, Kasper Skytte (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Rossetti, S (author)Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’...journal article 2022
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Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), Vodičková, Patricie (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including...journal article 2022
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Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...journal article 2022
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Calderon Franco, D. (author), Orschler, Laura (author), Lackner, Susanne (author), Agrawal, Shelesh (author), Weissbrodt, D.G. (author)The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemia has been one of the most difficult challenges humankind has recently faced. Wastewater-based epidemiology has emerged as a tool for surveillance and mitigation of potential viral outbreaks, circumventing biases introduced by clinical patient testing. Due to the situation...journal article 2022
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Kouba, V. (author), D, Vejmelkova (author), E, Zwolsman (author), K, Hurkova (author), K, Navratilova (author), M, Laureni (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Anammox bacteria enable efficient removal of nitrogen from sewage in processes involving partial nitritation and anammox (PN/A) or nitrification, partial denitrification, and anammox (N-PdN/A). In mild climates, anammox bacteria must be adapted to ≤15 °C, typically by gradual temperature decrease; however, this takes months or years. To...journal article 2022
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Cerruti, M. (author), Kim, J.H. (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Purple bacteria (PPB), anoxygenic photoorganoheterotrophic organisms with a hyper-versatile metabolism and high biomass yields over substrate, are promising candidates for the recovery of nutrient resources from wastewater. Infrared light is a pivotal parameter to control and design PPB-based resource recovery. However, the effects of light...journal article 2022