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Multi-scale in silico and ex silico mechanics of 3D printed cochlear implants for local drug delivery

Isaakidou, A. (author), Ganjian, M. (author), van Hoften, R. (author), Cruz Saldivar, M. (author), Leeflang, M.A. (author), Groetsch, A. (author), Wątroba, M. (author), Schwiedrzik, J. (author), Mirzaali, Mohammad J. (author), Apachitei, I. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

The currently available treatments for inner ear disorders often involve systemic drug administration, leading to suboptimal drug concentrations and side effects. Cochlear implants offer a potential solution by providing localized and sustained drug delivery to the cochlea. While the mechanical characterization of both the implants and their...

journal article 2024

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In vitro co-culture models for the assessment of orthopedic antibacterial biomaterials

Eijkel, B.I.M. (author), Apachitei, I. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

The antibacterial biofunctionality of bone implants is essential for the prevention and treatment of implant-associated infections (IAI). In vitro co-culture models are utilized to assess this and study bacteria-host cell interactions at the implant interface, aiding our understanding of biomaterial and the immune response against IAI without...

review 2024

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On-Demand Magnetically-Activated Drug Delivery from Additively Manufactured Porous Bone Implants to Tackle Antibiotic-Resistant Infections

Salandova, M. (author), Leeflang, M.A. (author), Klimopoulou, M. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

This study proposes a new concept for an on-demand drug releasing device intended for integration into additively manufactured (i.e., 3D printed) orthopedic implants. The system comprises a surface with conduits connected to a subsurface reservoir used for storage and on-demand release of antimicrobial agents, covered with a cap that prevents...

journal article 2024

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In Vivo Prevention of Implant-Associated Infections Caused by Antibiotic-Resistant Bacteria through Biofunctionalization of Additively Manufactured Porous Titanium

van Hengel, I.A.J. (author), van Dijk, B. (author), Modaresifar, K. (author), Hooning van Duyvenbode, J. Fred F. (author), Nurmohamed, F. Ruben H.A. (author), Leeflang, M.A. (author), Fluit, A.C. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Weinans, Harrie (author), Zadpoor, A.A. (author)

Additively manufactured (AM) porous titanium implants may have an increased risk of implant-associated infection (IAI) due to their huge internal surfaces. However, the same surface, when biofunctionalized, can be used to prevent IAI. Here, we used a rat implant infection model to evaluate the biocompatibility and infection prevention...

journal article 2023

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High-Precision 3D Printing of Microporous Cochlear Implants for Personalized Local Drug Delivery

Isaakidou, A. (author), Apachitei, I. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

Hearing loss is a highly prevalent multifactorial disorder affecting 20% of the global population. Current treatments using the systemic administration of drugs are therapeutically ineffective due to the anatomy of the cochlea and the existing blood–labyrinth barrier. Local drug delivery systems can ensure therapeutic drug concentrations locally...

journal article 2023

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The response of human macrophages to 3D printed titanium antibacterial implants does not affect the osteogenic differentiation of hMSCs

Garmendia Urdalleta, A. (author), van Poll, M.A. (author), Fahy, Niamh (author), Witte-Bouma, Janneke (author), van Wamel, Willem (author), Apachitei, I. (author), Zadpoor, A.A. (author), Fratila-Apachitei, E.L. (author), Farrell, Eric (author)

Macrophage responses following the implantation of orthopaedic implants are essential for successful implant integration in the body, partly through intimate crosstalk with human marrow stromal cells (hMSCs) in the process of new bone formation. Additive manufacturing (AM) and plasma electrolytic oxidation (PEO) in the presence of silver...

journal article 2023

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Preventing Antibiotic-Resistant Infections: Additively Manufactured Porous Ti6Al4V Biofunctionalized with Ag and Fe Nanoparticles

Putra, N.E. (author), Leeflang, M.A. (author), Ducret, Verena (author), Patrulea, Viorica (author), Fratila-Apachitei, E.L. (author), Perron, Karl (author), Ye, Hua (author), Zhou, J. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

Implant-associated infections are highly challenging to treat, particularly with the emergence of multidrug-resistant microbials. Effective preventive action is desired to be at the implant site. Surface biofunctionalization of implants through Ag-doping has demonstrated potent antibacterial results. However, it may adversely affect bone...

journal article 2022

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Fighting Antibiotic-Resistant Bacterial Infections by Surface Biofunctionalization of 3D-Printed Porous Titanium Implants with Reduced Graphene Oxide and Silver Nanoparticles

San, H. (author), Paresoglou, M. (author), Minneboo, M.B. (author), van Hengel, I.A.J. (author), Yilmaz, A. (author), Gonzalez Garcia, Y. (author), Fluit, Ad C. (author), Hagedoorn, P.L. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

Nanoparticles (NPs) have high multifunctional potential to simultaneously enhance implant osseointegration and prevent infections caused by antibiotic-resistant bacteria. Here, we present the first report on using plasma electrolytic oxidation (PEO) to incorporate different combinations of reduced graphene oxide (rGO) and silver (Ag) NPs on...

journal article 2022

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Antibacterial Titanium Implants Biofunctionalized by Plasma Electrolytic Oxidation with Silver, Zinc, and Copper: A Systematic Review

van Hengel, I.A.J. (author), Tierolf, M.W.A.M. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

Patients receiving orthopedic implants are at risk of implant-associated infections (IAI). A growing number of antibiotic-resistant bacteria threaten to hamper the treatment of IAI. The focus has, therefore, shifted towards the development of implants with intrinsic antibacterial activity to prevent the occurrence of infection. The use of Ag, Cu...

review 2021

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Inorganic Agents for Enhanced Angiogenesis of Orthopedic Biomaterials

Salandova, M. (author), van Hengel, I.A.J. (author), Apachitei, I. (author), Zadpoor, A.A. (author), van der Eerden, B.C.J. (author), Fratila-Apachitei, E.L. (author)

Aseptic loosening of a permanent prosthesis remains one of the most common reasons for bone implant failure. To improve the fixation between implant and bone tissue as well as enhance blood vessel formation, bioactive agents are incorporated into the surface of the biomaterial. This study reviews and compares five bioactive elements (copper,...

review 2021

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Osteogenic and antibacterial surfaces on additively manufactured porous Ti-6Al-4V implants: Combining silver nanoparticles with hydrothermally synthesized HA nanocrystals

Fazel, M. (author), Salimijazi, Hamid R. (author), Shamanian, Morteza (author), Minneboo, M.B. (author), Modaresifar, K. (author), van Hengel, I.A.J. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

The recently developed additively manufacturing techniques have enabled the fabrication of porous biomaterials that mimic the characteristics of the native bone, thereby avoiding stress shielding and facilitating bony ingrowth. However, aseptic loosening and bacterial infection, as the leading causes of implant failure, need to be further...

journal article 2021

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The effects of plasma electrolytically oxidized layers containing Sr and Ca on the osteogenic behavior of selective laser melted Ti6Al4V porous implants

van Hengel, I.A.J. (author), Lacin, M. (author), Minneboo, M.B. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

Surface biofunctionalization is frequently applied to enhance the functionality and longevity of orthopedic implants. Here, we investigated the osteogenic effects of additively manufactured porous Ti6Al4V implants whose surfaces were biofunctionalized using plasma electrolytic oxidation (PEO) in Ca/P-based electrolytes with or without...

journal article 2021

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Femoral prosthesis neck fracture following total hip arthroplasty: a systematic review

van Doesburg, P. G. (author), van Langelaan, E.J. (author), Apachitei, I. (author), Bénard, M. R. (author), Verdegaal, S. H.M. (author)

Purpose: Head-neck modularity was introduced into total hip arthroplasty to provide more intraoperative surgical options. However, modularity led to new problems, such as trunnionosis and fractures of the femoral prosthesis neck. The purpose of this study was to identify risk factors for hip neck fractures and to provide recommendations to...

review 2020

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Functionality-packed additively manufactured porous titanium implants

van Hengel, I.A.J. (author), Gelderman, F. S.A. (author), Athanasiadis, S. (author), Minneboo, M.B. (author), Weinans, Harrie (author), Fluit, A. C. (author), van der Eerden, B.C.J. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

The holy grail of orthopedic implant design is to ward off both aseptic and septic loosening for long enough that the implant outlives the patient. Questing this holy grail is feasible only if orthopedic biomaterials possess a long list of functionalities that enable them to discharge the onerous task of permanently replacing the native bone...

journal article 2020

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Multi-material additive manufacturing technologies for Ti-, Mg-, and Fe-based biomaterials for bone substitution

Putra, N.E. (author), Mirzaali, Mohammad J. (author), Apachitei, I. (author), Zhou, J. (author), Zadpoor, A.A. (author)

The growing interest in multi-functional metallic biomaterials for bone substitutes challenges the current additive manufacturing (AM, =3D printing) technologies. It is foreseeable that advances in multi-material AM for metallic biomaterials will not only allow for complex geometrical designs, but also improve their multi-functionalities by...

review 2020

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Immunomodulation of surface biofunctionalized 3D printed porous titanium implants

Razzi, F. (author), Fratila-Apachitei, E.L. (author), Fahy, N. (author), Bastiaansen-Jenniskens, Yvonne M. (author), Apachitei, I. (author), Farrell, E. (author), Zadpoor, A.A. (author)

Additive manufacturing (AM) techniques have provided many opportunities for the rational design of porous metallic biomaterials with complex and precisely controlled topologies that give rise to unprecedented combinations of mechanical, physical, and biological properties. These favorable properties can be enhanced by surface...

journal article 2020

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Self-defending additively manufactured bone implants bearing silver and copper nanoparticles

van Hengel, I.A.J. (author), Tierolf, M. W.A.M. (author), Valerio, V. P.M. (author), Minneboo, M.B. (author), Fluit, A. C. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

Effective preventive measures against implant-associated infection (IAI) are desperately needed. Therefore, the development of self-defending implants with intrinsic antibacterial properties has gained significant momentum. Biomaterials biofunctionalized with silver (Ag) have resulted in effective antibacterial biomaterials, yet regularly...

journal article 2020

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Biofunctionalization of selective laser melted porous titanium using silver and zinc nanoparticles to prevent infections by antibiotic-resistant bacteria

van Hengel, I.A.J. (author), Putra, N.E. (author), Tierolf, M. W.A.M. (author), Minneboo, M.B. (author), Fluit, A. C. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)

Antibiotic-resistant bacteria are frequently involved in implant-associated infections (IAIs), making the treatment of these infections even more challenging. Therefore, multifunctional implant surfaces that simultaneously possess antibacterial activity and induce osseointegration are highly desired in order to prevent IAIs. The incorporation...

journal article 2020

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Towards osteogenic and bactericidal nanopatterns?

Widyaratih, Dwisetya S. (author), Hagedoorn, P.L. (author), Otten, L.G. (author), Ganjian, M. (author), Tümer, N. (author), Apachitei, I. (author), Hagen, C.W. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

Recent discoveries have shown that nanopatterns with feature sizes ≤100 nm could direct stem cell fate or kill bacteria. These effects could be used to develop orthopedic implants with improved osseointegration and decreased chance of implant-associated infections. The quest for osteogenic and bactericidal nanopatterns is ongoing but no...

journal article 2019

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Nature helps: Toward bioinspired bactericidal nanopatterns

Ganjian, M. (author), Modaresifar, K. (author), Ligeon, Manon R.O. (author), Kunkels, Lorenzo B. (author), Tümer, N. (author), Angeloni, L. (author), Hagen, C.W. (author), Otten, L.G. (author), Hagedoorn, P.L. (author), Apachitei, I. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

Development of synthetic bactericidal surfaces is a drug-free route to the prevention of implant-associated infections. Surface nanotopographies with specific dimensions have been shown to kill various types of bacterial strains through a mechanical mechanism, while regulating stem cell differentiation and tissue regeneration. The effective...

journal article 2019