Fu, Rongjie (author), He, Wei (author), Dou, Jinzhuang (author), Villarreal, Oscar D. (author), Bedford, Ella (author), Wang, Helen (author), Hou, Connie (author), Zhang, Liang (author), Depken, S.M. (author) The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (gRNA) and the targeted DNA, yet the sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore the sequence determinants governing CRISPR/Cas9 specificity, here we describe a dual-target system to...
journal article 2022
