Zhou, J. (author), Olson, D.G. (author), Argyros, D.A. (author), Deng, Y. (author), Van Gulik, W.M. (author), Van Dijken, J.P. (author), Lynd, L.R. (author) Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity....
journal article 2013
