H₂O₂ Production at Low Overpotentials for Electroenzymatic Halogenation Reactions

Various enzymes utilize hydrogen peroxide as an oxidant. Such “peroxizymes” are potentially very attractive catalysts for a broad range of oxidation reactions. Most peroxizymes, however, are inactivated by an excess of H₂O₂. The electrochemical reduction of oxygen can be used as an in situ generation method for hydrogen...

Efficient Aerobic Oxidation of trans-2-Hexen-1-ol using the Aryl Alcohol Oxidase from Pleurotus eryngii

The selective oxidation of trans-2-hexen-1-ol to the corresponding aldehyde using a recombinant aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. Especially using the two liquid phase system to overcome solubility and product inhibition issues enabled to achieve more than 2.200.000 catalytic turnovers for the production enzyme...

Biocatalytic synthesis of the Green Note trans-2-hexenal in a continuous-flow microreactor

The biocatalytic preparation of trans-hex-2-enal from trans-hex-2-enol using a novel aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. As O₂-dependent enzyme PeAAOx-dependent reactions are generally plagued by the poor solubility of O₂ in aqueous media and mass transfer limitations resulting in poor...

Towards environmentally acceptable synthesis of chiral α-hydroxy ketones via oxidase-lyase cascades

The one-pot multistep enzymatic oxidation of aliphatic and benzylic alcohols to the corresponding aldehydes combined with their subsequent carboligation to chiral α-hydroxy ketones has been exemplarily evaluated in terms of being a “green” biocatalytic approach. Besides the potential to start from bio-derived alcohols, this concept avoids the...