Luo, Zhiyuan (author), Qiao, Li (author), Chen, Haomin (author), Mao, Zhili (author), Wu, Shujiao (author), Ma, Bianqin (author), Xie, Tian (author), Wang, Anming (author), Pei, Xiaolin (author), Sheldon, R.A. (author) The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...
journal article 2024
