Process for creating a double-stranded polyribonucleotide sequence with terminal overhang, as well as a process for creating a double-stranded polynucleotide construct and an application

The invention relates to a process for creating double-stranded RNA (16) having a terminal overhang. In accordance with the invention a DNA amplification is used for this purpose, followed by a transcription of the amplified DNA. When amplifying the DNA, primer pairs are used and care is taken that additional sequences (18, 19) are present that...