A novel experimental method to determine substrate uptake kinetics of gaseous substrates applied to the carbon monoxide-fermenting Clostridium autoethanogenum

Syngas fermentation has gained momentum over the last decades. The cost-efficient design of industrial-scale bioprocesses is highly dependent on quantitative microbial growth data. Kinetic and stoichiometric models for syngas-converting microbes exist, but accurate experimental validation of the derived parameters is lacking. Here, we...

Poison to Products: On harnessing the power of microorganisms to convert waste streams into new chemicals

One of the main challenges society currently deals with is the depletion of fossil fuels. To navigate this issue, we must embrace the concept of circularity and turn waste into a resource. Waste streams are omnifarious and their conversion into new chemical building blocks is not always trivial. Luckily, we can take a look at nature’s problem...

Overflow metabolism at the thermodynamic limit of life: How carboxydotrophic acetogens mitigate carbon monoxide toxicity

Carboxydotrophic metabolism is gaining interest due to its applications in gas fermentation technology, enabling the conversion of carbon monoxide to fuels and commodities. Acetogenic carboxydotrophs play a central role in current gas fermentation processes. In contrast to other energy-rich microbial substrates, CO is highly toxic, which...

Physiological and stoichiometric characterization of ethanol-based chain elongation in the absence of short-chain carboxylic acids

Hexanoate is a valuable chemical that can be produced by microorganisms that convert short-chain- to medium-chain carboxylic acids through a process called chain elongation. These microorganisms usually produce mixtures of butyrate and hexanoate from ethanol and acetate, but direct conversion of ethanol to hexanoate is theoretically possible....

Earlier chronotype in patients with rheumatoid arthritis

Objectives: Rheumatoid arthritis (RA) patients show an earlier circadian rhythm (i.e. serum melatonin peaks earlier during the night, indicating an earlier timing of the internal circadian pacemaker). In the current study, we examined whether the chronotype, which is influenced by the circadian rhythm, is also earlier. In addition, we...

Product Inhibition and pH Affect Stoichiometry and Kinetics of Chain Elongating Microbial Communities in Sequencing Batch Bioreactors

Anaerobic microbial communities can produce carboxylic acids of medium chain length (e.g., caproate, caprylate) by elongating short chain fatty acids through reversed β-oxidation. Ethanol is a common electron donor for this process. The influence of environmental conditions on the stoichiometry and kinetics of ethanol-based chain elongation...

Risk Management for the Underground Car Park Kruisplein

In the preparation and construction stages of the underground car park beneath Kruisplein with diaphragm walls, risk management played an important role. After a description of the structure and the construction, the preventive measures that were invented and carried out are summarised. These relate to the organisation of the project ...

Ampyx power market development

Polarization-dependent quantum beats of four-wave mixing in the Luttinger model for bulk semiconductors

Design principles for flexible pavements: A computational model for granular bases

De distributie van een groter assortiment "Olvarit Babyvoeding"

Het ontwerpen van pneumatische transportinstallaties

Ontwerp van een verdraaibare as-schildverbinding in trommels van bandtransporteurs

Vermenigvuldigers voor de analogon-rekenmachine van de sub-afdeling Vliegtuigbouwkunde der Technische Hogeschool te Delft