The tungsten metallome of Pyrococcus furiosus

The tungsten metallome of the hyperthermophilic archaeon Pyrococcus furiosus has been investigated using electroanalytical metal analysis and native–native 2D-PAGE with the radioactive tungsten isotope W-187 (t1/2 = 23.9 h). P. furiosus cells have an intracellular tungsten concentration of 29 mM, of which ca. 30% appears to be free tungsten,...

A steady-state and pre-steady-state kinetics study of the tungstoenzyme formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus

Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus is a homotetrameric protein with one tungstodipterin and one [4Fe–4S] cubane per 69-kDa subunit. The enzyme kinetics have been studied under steady-state conditions at 80 °C and pre-steady state conditions at 50 °C, in the latter case via monitoring of the relatively weak (? ? 2 mM...

A steady-state and pre-steady-state kinetics study of the tungstoenzyme formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus

Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus is a homotetrameric protein with onetungstodipterin and one [4Fe–4S] cubane per 69-kDa subunit. The enzyme kinetics have been studied understeady-state conditions at 80 C and pre-steady state conditions at 50 C, in the latter case via monitoring of the relatively weak (e ~ 2 mM–1 cm...

WOR5, a Novel Tungsten-Containing Aldehyde Oxidoreductase from Pyrococcus furiosus with a Broad Substrate Specificity