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Liu, Z. (author), van Veen, E.N.W. (author), Sanchez González, H. (author), Solano Hermosilla, B.P. (author), Palmero Moya, F.J.P.M. (author), Mc Cluskey, K.A. (author), Ramirez Montero, D.F. (author), van Laar, T. (author), Dekker, N.H. (author)Integrated single-molecule force-fluorescence spectroscopy setups allow for simultaneous fluorescence imaging and mechanical force manipulation and measurements on individual molecules, providing comprehensive dynamic and spatiotemporal information. Dual-beam optical tweezers (OT) combined with a confocal scanning microscope form a force...journal article 2024
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Sanchez González, H. (author), Liu, Z. (author), van Veen, E.N.W. (author), van Laar, T. (author), Diffley, John F.X. (author), Dekker, N.H. (author)Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (ORC). In yeast, ORC is a dynamic protein that diffuses rapidly along DNA, unless halted by origin...journal article 2023