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Loeff, L. (author), Kerssemakers, J.W.J. (author), Joo, C. (author), Dekker, C. (author)Single-molecule techniques allow the visualization of the molecular dynamics of nucleic acids and proteins with high spatiotemporal resolution. Valuable kinetic information of biomolecules can be obtained when the discrete states within single-molecule time trajectories are determined. Here, we present a fast, automated, and bias-free step...journal article 2021
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Resolving Chemical Modifications to a Single Amino Acid within a Peptide Using a Biological NanoporeRestrepo Perez, L. (author), Huang, Gang (author), Bohländer, Peggy R. (author), Worp, N.M. (author), Eelkema, R. (author), Maglia, Giovanni (author), Joo, C. (author), Dekker, C. (author)While DNA sequencing is now amply available, fast, and inexpensive, protein sequencing remains a tremendous challenge. Nanopores may allow for developing a protein sequencer with single-molecule capabilities. As identification of 20 different amino acids currently presents an unsurmountable challenge, fingerprinting schemes are pursued, in...journal article 2019
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Shi, X. (author), Verschueren, D.V. (author), Dekker, C. (author)Plasmon resonance biosensors provide ultimate sensitivity at the single-molecule level. This sensitivity is, however, associated with a nanometer-sized confined hotspot, and molecular transport toward the sensor relies on inefficient diffusion. Here, we combine a plasmonic nanoantenna with a solid-state nanopore and demonstrate that single...journal article 2018
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Heerema, S.J. (author), Vicarelli, L. (author), Pud, S. (author), Schouten, R.N. (author), Zandbergen, H.W. (author), Dekker, C. (author)Many theoretical studies predict that DNA sequencing should be feasible by monitoring the transverse current through a graphene nanoribbon while a DNA molecule translocates through a nanopore in that ribbon. Such a readout would benefit from the special transport properties of graphene, provide ultimate spatial resolution because of the single...journal article 2018
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Plesa, C. (author)Solid-state nanopores are small nanometer-scale holes in thin membranes. When used to separate two chambers containing salt solution, any biomolecule passing from one chamber to the other is forced to pass through the pore constriction. An electric field applied across the membrane is used to create an ionic current and electrophoretically drive...doctoral thesis 2015
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Kowalczyk, S.W. (author)Solid-state nanopores, nanometer-size holes in a thin synthetic membrane, are a versatile tool for the detection and manipulation of charged biomolecules. This thesis describes mostly experimental work on DNA translocation through solid-state nanopores, which we study at the single-molecule level. In particular, we demonstrate length-wise ...doctoral thesis 2011
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Smeets, R.M.M. (author)This thesis describes experimental work on a novel type of devices capable of detecting single-(bio)molecules; nanometer-sized pores, or nanopores. Individual nanopores are placed in between two electrolyte-filled liquid compartments and (bio)molecules are electrophoretically driven through them. During passage of a single (bio)molecule the...doctoral thesis 2008
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Storm, A.J. (author)This thesis describes the fabrication and use of nanostructures to study the physical properties of individual DNA molecules. We report DNA translocation experiments through solid-state nanopores with a diameter of about 10 nm. DNA ranging in length from 2000 to 48000 base pairs was detected and we find that the translocation time scales as a...doctoral thesis 2004