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Singh, Abhishek A. (author), Schuurman, Karianne (author), Nevedomskaya, Ekaterina (author), Stelloo, Suzan (author), Linder, Simon (author), Droog, Marjolein (author), Kim, Yongsoo (author), Sanders, Joyce (author), van der Poel, Henk (author), Bergman, Andries M. (author), Wessels, L.F.A. (author), Zwart, Wilbert (author)Chromatin immunoprecipitation (ChIP)-seq analyses of transcription factors in clinical specimens are challenging due to the technical limitations and low quantities of starting material, often resulting in low enrichments and poor signal-to-noise ratio. Here, we present an optimized protocol for transcription factor ChIP-seq analyses in human...journal article 2019
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Stelloo, Suzan (author), Nevedomskaya, Ekaterina (author), Kim, Yongsoo (author), Schuurman, Karianne (author), Valle-Encinas, Eider (author), Lobo, João (author), Krijgsman, Oscar (author), Peeper, Daniel Simon (author), Chang, Seiwon Laura (author), Wessels, L.F.A. (author)The Androgen Receptor (AR) is the key-driving transcription factor in prostate cancer, tightly controlled by epigenetic regulation. To date, most epigenetic profiling has been performed in cell lines or limited tissue samples. Here, to comprehensively study the epigenetic landscape, we perform RNA-seq with ChIP-seq for AR and histone...journal article 2018