Compartmentalized cross-linked enzyme nano aggregates (c-CLEnAs) toward pharmaceutical transformations

A new immobilization strategy using compartmentalized nanoreactors is herein reported for two biocatalytic processes: (1)N-acetylneuraminate lyase (NAL) is internalized in NAL-c-CLEnAs and used in a continuous flow aldol condensation ofN-acetyl-d-mannosamine with sodium pyruvate toN-acetylneuraminic acid; (2) two hydroxysteroid dehydrogenases...

Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite

The hydroxynitrile lyase from Prunus amygdalus was immobilized on Celite R-633. The immobilized enzyme could successfully be utilized in buffer saturated MTBE and excellent conversions of benzaldehyde to R-mandelonitrile were observed. No leaching occurred. To achieve high enantioselectivities, the suppression of the undesired background...

Michael hydratase alcohol dehydrogenase or just alcohol dehydrogenase?

The Michael hydratase – alcohol dehydrogenase (MhyADH) from Alicycliphilus denitrificans was previously identified as a bi-functional enzyme performing a hydration of ?,?-unsaturated ketones and subsequent oxidation of the formed alcohols. The investigations of the bi-functionality were based on a spectrophotometric assay and an activity...

TUD-1: Synthesis and application of a versatile catalyst, carrier, material...

The three-dimensional sponge-like mesoporous material TUD-1 is straightforward to prepare. Its synthesis can readily be modified to introduce metals into the framework of TUD-1, imparting many different catalytic activities. M-TUD-1 catalysts have proven to be very active, unlimited by diffusion and very stable. By combining two metals into one...