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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Bale, Nicol J. (author), Sinnighe Damsté, Jaap S. (author), Kublanov, Ilya V. (author)Beta-mannans are insoluble plant polysaccharides with beta-1,4-linked mannose as the backbone. We used three forms of this polysaccharide, namely, pure mannan, glucomannan, and galactomannan, to enrich haloarchaea, which have the ability to utilize mannans for growth. Four mannan-utilizing strains obtained in pure cultures were closely related...journal article 2024
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Elcheninov, Alexander G. (author), Ugolkov, Yaroslav A. (author), Elizarov, Ivan M. (author), Klyukina, Alexandra A. (author), Kublanov, Ilya V. (author), Sorokin, Dimitry Y. (author)Extremely halophilic archaea are one of the principal microbial community components in hypersaline environments. The majority of cultivated haloarchaea are aerobic heterotrophs using peptides or simple sugars as carbon and energy sources. At the same time, a number of novel metabolic capacities of these extremophiles were discovered recently...journal article 2023
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Merkel, Alexander Y. (author), Bale, Nocole J. (author), Sininghe-Damste, Jaap S. (author), Kublanov, Ilya V. (author)Two strains of neutrophilic haloaloarchaea were selectively enriched from hypersaline lakes in southwestern Siberia using β-1,3-glucans as a substrate. The strains were nearly identical in their phenotypes and according to<br/>phylogenomic analysis, and represent a distant novel species group in the genus Halapricum of the family Haloarculaceae....journal article 2023
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Merkel, Alexander Y. (author), Bale, Nicole J. (author), Sininghe-Damste, Jaap (author), Kublanov, Ilya V. (author)The authors regret that there is a mistake in the strain collection number: instead of UQ 51487 it should be UQM 51487 in the protologue Table 3 of their paper. The corrected protologue Table is presented below.journal article 2023
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Zhilina, Tatjana N. (author), Sorokin, Dimitry Y. (author), Toschakov, Stepan V. (author), Kublanov, Ilya V. (author), Zavarzina, Daria G. (author)Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702 <sup>T</sup> is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum...journal article 2023
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Khizhniak, Tatjana V. (author), Koenen, Michel (author), Bale, Nicole J. (author), Damsté, Jaap S.Sinninghe (author), Kublanov, Ilya V. (author)Several pure cultures of alkaliphilic haloaloarchaea were enriched and isolated from hypersaline soda lakes in southwestern Siberia using amylopectin and fructans as substrates. Phylogenomic analysis placed the isolates into two distinct groups within the class Halobacteria. Four isolates forming group 1 were closely related to a recently...journal article 2022
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Khijniak, Tatiana V. (author), Kolganova, Tatiana V. (author), Kublanov, Ilya V. (author)Extremely halophilic archaea (haloarchaea) of the class Halobacteria is<br/>a dominant group of aerobic heterotrophic prokaryotic communities in<br/>salt-saturated habitats, such as salt lakes and solar salterns. Most of the<br/>pure cultures of haloarchaea were enriched, isolated, and cultivated on<br/>rich soluble substrates such as amino...journal article 2022
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Sorokin, Dimitry Y. (author), Khijniak, Tatjana V. (author), Zakharycheva, Alicia P. (author), Elcheninov, Alexander G. (author), Hahnke, Richard L. (author), Boueva, Olga V. (author), Ariskina, Elena V. (author), Bunk, Boyke (author), Kublanov, Ilya V. (author), Evtushenko, Lyudmila I. (author)A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22<sup>T</sup>, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia. The isolate was facultatively alkaliphilic, growing at pH 6.5–10.5 (optimum at 7.3–9.0) and highly salt-tolerant, tolerating up to 3 M total Na<sup>+</sup> as...journal article 2021
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Sorokin, Dimitry Y. (author), Elcheninov, Alexander G. (author), Toshchakov, Stepan V. (author), Bale, Nicole J. (author), Sinninghe Damsté, Jaap S. (author), Khijniak, Tatiana V. (author), Kublanov, Ilya V. (author)Two groups of alkaliphilic haloarchaea from hypersaline alkaline lakes in Central Asia, Egypt and North America were enriched and isolated in pure culture using chitin as growth substrate. These cultures, termed AArcht, were divided into two groups: group 1 which includes eleven isolates from highly alkaline soda lakes and group 2 which...journal article 2019
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Sorokin, Dimitry Y. (author), Khijniak, Tatiana V. (author), Elcheninov, Alexander G. (author), Toshchakov, Stepan V. (author), Kostrikina, Nadezhda A. (author), Bale, Nicole J. (author), Sinninghe Damsté, Jaap S. (author), Kublanov, Ilya V. (author)An extremely halophilic euryarchaeon, strain HArcel1T, was enriched and isolated in pure culture from the surface brines and sediments of hypersaline athalassic lakes in the Kulunda Steppe (Altai region, Russia) using amorphous cellulose as the growth substrate. The colonies of HArcel1T are pale-orange, and form large zones of cellulose...journal article 2019
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Sorokin, Dimitry Y. (author), Khijniak, Tatiana V. (author), Kostrikina, Nadezhda A. (author), Elcheninov, Alexander G. (author), Toshchakov, Stepan V. (author), Bale, Nicole J. (author), Damsté, Jaap S.Sinninghe (author), Kublanov, Ilya V. (author)Six strains of extremely halophilic and alkaliphilic euryarchaea were enriched and isolated in pure culture from surface brines and sediments of hypersaline alkaline lakes in various geographical locations with various forms of insoluble cellulose as growth substrate. The cells are mostly flat motile rods with a thin monolayer cell wall while...journal article 2018
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Sorokin, Dimitry Y. (author), Muntyan, Maria S. (author), Toshchakov, Stepan V. (author), Korzhenkov, Aleksei (author), Kublanov, Ilya V. (author)Stable development of a heterotrophic bacterial satellite with a peculiar cell morphology has been observed in several enrichment cultures of haloalkaliphilic benthic filamentous cyanobacteria from a hypersaline soda lake in Kulunda Steppe (Altai, Russia). The organism was isolated in pure culture (strain Omega) using sonicated cyanobacterial...journal article 2018
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Sorokin, Dimitry Y. (author), Khijniak, Tatiana V. (author), Galinski, Erwin A. (author), Kublanov, Ilya V. (author)Two proteolytic bacterial strains, BSker2<sup>T</sup>and BSker3<sup>T</sup>, were enriched from sediments of hypersaline alkaline lakes in Kulunda Steppe (Altai, Russia) with chicken feathers as substrate, followed by pure culture isolation on hypersaline alkaline media with casein. The cells were non-motile, filamentous, flexible rods. The...journal article 2017
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Sorokin, Dimitry Y. (author), Messina, Enzo (author), Smedile, Francesco (author), Roman, Pawel (author), Damsté, Jaap S.Sinninghe (author), Ciordia, Sergio (author), Mena, Maria Carmen (author), Ferrer, Manuel (author), Golyshin, Peter N. (author), Kublanov, Ilya V. (author), Samarov, Nazar I. (author), Toshchakov, Stepan V. (author), La Cono, Violetta (author), Yakimov, Michail M. (author)Hypersaline anoxic habitats harbour numerous novel uncultured archaea whose metabolic and ecological roles remain to be elucidated. Until recently, it was believed that energy generation via dissimilatory reduction of sulfur compounds is not functional at salt saturation conditions. Recent discovery of the strictly anaerobic acetotrophic...journal article 2017
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Sorokin, Dimitry Y. (author), Kolganova, Tatiana V. (author), Khijniak, Tatiana V. (author), Jones, Brian E. (author), Kublanov, Ilya V. (author)Alkaline saline soils, known also as ''soda solonchaks'', represent a natural soda habitat which differs from soda lake sediments by higher aeration and lower humidity. The microbiology of soda soils, in contrast to the more intensively studied soda lakes, remains poorly explored. In this work we investigate the diversity of culturable...journal article 2017
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Sorokin, Dimitry Y. (author), Kublanov, Ilya V. (author), Khijniak, Tatiana V. (author)Brine samples from Kulunda Steppe soda lakes (Altai, Russia) were inoculated into a hypersaline alkaline mineral medium with β-keratin (chicken feather) as a substrate. The micro-organisms dominating the enrichment culture were isolated by limiting serial dilution on the same medium with casein as a substrate. The cells of strain BSker1<sup>T...journal article 2017
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Sorokin, Dimitry Y. (author), Kublanov, Ilya V. (author), Yakimov, Mikhail M. (author), Rijpstra, W. Irene C (author), Sinninghe Damsté, Jaap S. (author)Anaerobic enrichments with acetate as electron donor and carbon source, and elemental sulfur as electron acceptor at 4 M NaCl using anaerobic sediments and brines from several hypersaline lakes in Kulunda Steppe (Altai, Russia) resulted in isolation in pure culture of four strains of obligately anaerobic haloarchae growing exclusively by...journal article 2016
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Messina, Enzo (author), Sorokin, Dimitry Y. (author), Kublanov, Ilya V. (author), Toshchakov, Stepan (author), Lopatina, Anna (author), Arcadi, Erika (author), Smedile, Francesco (author), La Spada, Gina (author), La Cono, Violetta (author), Yakimov, Michail M. (author)Strain M27-SA2 was isolated from the deep-sea salt-saturated anoxic lake Medee, which represents one of the most hostile extreme environments on our planet. On the basis of physiological studies and phylogenetic positioning this extremely halophilic euryarchaeon belongs to a novel genus 'Halanaeroarchaeum' within the family Halobacteriaceae....journal article 2016
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Sorokin, D.Y. (author), Toshchakov, S.V. (author), Kolganova, T.V. (author), Kublanov, I.V. (author)Until recently, extremely halophilic euryarchaeota were considered mostly as aerobic heterotrophs utilizing simple organic compounds as growth substrates. Almost nothing is known on the ability of these prokaryotes to utilize complex polysaccharides, such as cellulose, xylan, and chitin. Although few haloarchaeal cellulases and chitinases were...journal article 2015
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Dominova, I.N. (author), Sorokin, D.Y. (author), Kublanov, I.V. (author), Patrushev, M.V. (author), Toshchakova, S.V. (author)The complete genome sequence of a novel halophilic archaeon, Salinarchaeum sp. strain HArcht-Bsk1T, was determined using next-generation sequencing. The genome comprises a 3,255,260-bp circular chromosome with a G+C content of 66.7%. Automatic annotation of the genome revealed a single rRNA operon, 45 tRNAs, and 3,013 protein-coding gene sequences.journal article 2013