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Wijsman, Jeroen W.M. (author), Prins, Theo C. (author), Moons, J. J.Simeon (author), Herman, P.M.J. (author)Sand nourishment is a widespread management strategy to protect sandy coasts against erosion. Nourishments can impact the benthic fauna directly by burial and indirectly by changing environmental conditions such as bottom shear stress and/or sediment composition (grain size distribution). The macrobenthic community in soft-bottom environments...journal article 2023
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Boonekamp, F.J. (author), Knibbe, E. (author), Vieira Lara, M.A. (author), Wijsman, M. (author), Luttik, M.A.H. (author), van Eunen, Karen (author), den Ridder, M.J. (author), Pabst, Martin (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)Although transplantation of single genes in yeast plays a key role in elucidating gene functionality in metazoans, technical challenges hamper humanization of full pathways and processes. Empowered by advances in synthetic biology, this study demonstrates the feasibility and implementation of full humanization of glycolysis in yeast. Single...journal article 2022
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Albalak, Gali (author), Stijntjes, M. (author), Wijsman, Carolien A. (author), Slagboom, P. Eline (author), van der Ouderaa, Frans J. (author), Mooijaart, Simon P. (author), van Heemst, Diana (author), Noordam, Raymond (author)Background: Little is known about the impact of timing as opposed to frequency and intensity of daily physical activity on metabolic health. Therefore, we assessed the association between accelerometery-based daily timing of physical activity and measures of metabolic health in sedentary older people. Methods: Hourly mean physical activity...journal article 2022
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Wijsman, Jeroen W.M. (author), Craeymeersch, Johan A. (author), Herman, P.M.J. (author)Epifauna and infauna are often sampled by different types of sampling devices including grabs, and dredges, depending on various factors such as local environmental conditions, research questions and available budget. Because grabs and dredges have a different species-specific selectivity, both methods could be complementary but for some...journal article 2022
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Herman, P.M.J. (author), Moons, J. J.Simeon (author), Wijsman, Jeroen W.M. (author), Luijendijk, Arjen (author), Ysebaert, Tom (author)The Sand Motor is a very large (20 million m<sup>3</sup>) nourishment constructed along the coast in The Netherlands. The huge volume of sand is redistributed along the coast by natural forces stemming from tidal currents and waves. For environmental evaluation of this large construction, the benthic subtidal fauna has been sampled prior to...journal article 2021
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Wijsman, M. (author), Swiat, M.A. (author), Marques, W.L. (author), Hettinga, Johanna K. (author), van den Broek, M.A. (author), Torre Cortés, Pilar de la (author), Mans, R. (author), Pronk, J.T. (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)Hexose transporter-deficient yeast strains are valuable testbeds for the study of sugar transport by native and heterologous transporters. In the popular Saccharomyces cerevisiae strain EBY.VW4000, deletion of 21 transporters completely abolished hexose transport. However, repeated use of the LoxP/Cre system in successive deletion rounds also...journal article 2018
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Mans, R. (author), Wijsman, M. (author), Daran-Lapujade, P.A.S. (author), Daran, J.G. (author)Here, two methods are described for efficient genetic modification of Saccharomyces cerevisiae using CRISPR/Cas9. The first method enables the modification of a single genetic locus using in vivo assembly of a guide RNA (gRNA) expression plasmid without the need for prior cloning. A second method using in vitro assembled plasmids that could...journal article 2018
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Mans, R. (author), Else-Hassing, J. (author), Wijsman, M. (author), Giezekamp, Annabel (author), Pronk, J.T. (author), Daran, J.G. (author), van Maris, A.J.A. (author)CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25...journal article 2017
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Swiat, M.A. (author), Dashko, S. (author), den Ridder, M.J. (author), Wijsman, M. (author), van der Oost, John (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)Cpf1 is a new class II family of CRISPR-Cas RNA-programmable endonucleases with unique features that make it a very attractive alternative or complement to Cas9 for genome engineering. Using constitutively expressed Cpf1 from Francisella novicida, the present study demonstrates that FnCpf1 can mediate RNA-guided DNA cleavage at targeted...journal article 2017
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Salazar, A.N. (author), Gorter de Vries, A.R. (author), van den Broek, M.A. (author), Wijsman, M. (author), de la Torre, P. (author), Brickwedde, A. (author), Brouwers, N. (author), Daran, J.G. (author), Abeel, T.E.P.M.F. (author)The haploid Saccharomyces cerevisiae strain CEN.PK113–7D is a popular model system for metabolic engineering and systems biology research. Current genome assemblies are based on short-read sequencing data scaffolded based on homology to strain S288C. However, these assemblies contain large sequence gaps, particularly in subtelomeric regions, and...journal article 2017
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Mans, R. (author), Van Rossum, H.M. (author), Wijsman, M. (author), Backx, A. (author), Kuijpers, N.G.A. (author), van den Broek, M. (author), Daran-Lapujade, P.A.S. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author), Daran, J.G. (author)A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently been developed. However, especially when multiple genetic manipulations are required, strain construction is still a time-consuming process. This study describes new CRISPR/Cas9-based approaches for easy, fast strain construction in yeast and explores their...journal article 2015
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Birklund, J. (author), Wijsman, J.W.M. (author)The expected major increase in marine aggregate extraction in the future will increase the spatial and temporal environmental impacts. Coastal zone management and interdisciplinary surveys and assessments will become increasingly important in order to minimise impacts of largescale sand mining on marine resources and to resolve conflicts against...report 2005
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- Wijsman, J.W.M (author) report 2005
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- Wijsman, J.W.M. (author), Verhage, L. (author) report 2004
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- Wijsman, J.W.M. (author) report 2003
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Peters, B.G.T.M. (author), Liek, G.A. (author), Wijsman, J.W.M. (author), Kuijper, M.W.M. (author), Van Eck, G.T. (author)In 1995 kwamen Vlaanderen en Nederland overeen dat de vaargeul naar Antwerpen verruimd zou worden tot 48/43/38 voet. Dit betekent dat schepen met een maximale diepgang van 48 voet (1 voet is circa 0,3 meter) in één getij naar Antwerpen kunnen varen en dat schepen met een maximale diepgang van 43 voet in één getij vanuit Antwerpen de...report 2003
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Stikvoort, E. (author), Berrevoets, C.M. (author), Kuijper, M. (author), Lefèvre, F. (author), Liek, G.J. (author), Lievaart, M.A. (author), Van Maldegem, D. (author), Meininger, P.L. (author), Peters, B. (author), Pouwer, A.J. (author), Schippers, H.K. (author), Wijsman, J.W.M. (author)Het voor u liggende MOVE Hypothesendocument 2003 is door RIKZ in opdracht van directie Zeeland van Rijkswaterstaat opgesteld. Aan de basis van dit rapport staan zeer vele gegevens die in de loop van soms al tientallen jaren in de Westerschelde verzameld zijn en nog verzameld worden. Het betreft fysische, biologische en chemische informatie, met...report 2003
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Wijsman, J. (author)In de zomer van 1997 is men begonnen met het verdiepen van de Westerschelde ten behoeve van de scheepvaart naar Antwerpen. De verdiepingswerkzaamheden hebben invloed op de hydrologie, morfologie, chemie en biologie van de Westerschelde. Om deze omvang van veranderingen in kaart te brengen is het project Monitoring Verdieping Westerschelde (MOVE)...report 2003
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- Wijsman, J.W.M. (author) report 2001
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- Wijsman, M.R. (author), Van Dijken, J.P. (author), Van Kleeff, B.H.A. (author), Scheffers, W.A. (author) journal article 1984