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Coupling extracellular glycan composition with metagenomic data in papermill and brewery anaerobic granular sludges

Doloman, Anna (author), de Bruin, S. (author), van Loosdrecht, Mark C.M. (author), Sousa, Diana Z. (author), Lin, Y. (author)

Glycans are crucial for the structure and function of anaerobic granular sludge in wastewater treatment. Yet, there is limited knowledge regarding the microorganisms and biosynthesis pathways responsible for glycan production. In this study, we analysed samples from anaerobic granular sludges treating papermill and brewery wastewater,...

journal article 2024

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Anionic extracellular polymeric substances extracted from seawater-adapted aerobic granular sludge

Chen, L.M. (author), Beck, Paula (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Abstract: Anionic polymers, such as heparin, have been widely applied in the chemical and medical fields, particularly for binding proteins (e.g., fibroblast growth factor 2 (FGF-2) and histones). However, the current animal-based production of heparin brings great risks, including resource shortages and product contamination. Recently,...

journal article 2024

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The water-soluble fraction of extracellular polymeric substances from a resource recovery demonstration plant: characterization and potential application as an adhesive

Chen, Le Min (author), Erol, Özlem (author), Choi, Young Hae (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Currently, there is a growing interest in transforming wastewater treatment plants (WWTPs) into resource recovery plants. Microorganisms in aerobic granular sludge produce extracellular polymeric substances (EPS), which are considered sustainable resources to be extracted and can be used in diverse applications. Exploring applications in other...

journal article 2024

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Identification and degradation of structural extracellular polymeric substances in waste activated sludge via a polygalacturonate-degrading consortium

Hu, Zhi Yi (author), Lin, Yi Peng (author), Wang, Qing Ting (author), Zhang, Yi Xin (author), Tang, Jie (author), Wang, Shuai (author), van Loosdrecht, Mark C.M. (author), Zeng, Raymond Jianxiong (author), Zhang, Fang (author)

By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and...

journal article 2023

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Alterations of Glycan Composition in Aerobic Granular Sludge during the Adaptation to Seawater Conditions

Chen, L.M. (author), Keisham, Sunanda (author), Tateno, Hiroaki (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Bacteria can synthesize a diverse array of glycans, being found attached to proteins and lipids or as loosely associated polysaccharides to the cells. The major challenge in glycan analysis in environmental samples lies in developing high-throughput and comprehensive characterization methodologies to elucidate the structure and monitor the...

journal article 2023

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Highly efficient carbon assimilation and nitrogen/phosphorus removal facilitated by photosynthetic O₂ from algal-bacterial aerobic granular sludge under controlled DO/pH operation

Li, Zejiao (author), Wang, Jixiang (author), Liu, Jialin (author), Chen, Xingyu (author), Lei, Zhongfang (author), Yuan, Tian (author), Lee, Duu Jong (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)

Reducing CO₂ emission and energy consumption is crucial for the sustainable management of wastewater treatment plants (WWTPs). In this study, an algal-bacterial aerobic granular sludge (AGS) system was developed for efficient carbon (C) assimilation and nitrogen (N)/phosphorus (P) removal without the need for mechanical aeration....

journal article 2023

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Sialylation and Sulfation of Anionic Glycoconjugates Are Common in the Extracellular Polymeric Substances of Both Aerobic and Anaerobic Granular Sludges

Chen, L.M. (author), de Bruin, S. (author), Pronk, M. (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Anaerobic and aerobic granular sludge processes are widely applied in wastewater treatment. In these systems, microorganisms grow in dense aggregates due to the production of extracellular polymeric substances (EPS). This study investigates the sialylation and sulfation of anionic glyconconjugates in anaerobic and aerobic granular sludges...

journal article 2023

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Enhancement of fire resistance and mechanical performance of polypropylene composites containing cellulose fibres and extracellular biopolymers from wastewater sludge

Kim, Nam Kyeun (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

In the present research, a bio-based flame retardant (FR) was prepared using a biopolymer derived from wastewater sludge to improve the fire performance of polypropylene (PP). Extracellular polymeric substances (EPS), which were extracted from wastewater aerobic granular sludge, were absorbed into cellulose-based fibres, such as flax and...

journal article 2023

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Turnover of the extracellular polymeric matrix of granules performing biological phosphate removal

Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)

Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...

journal article 2023

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Metaproteomics, metagenomics and 16S rRNA sequencing provide different perspectives on the aerobic granular sludge microbiome

Kleikamp, H.B.C. (author), Grouzdev, Denis (author), Schaasberg, P. (author), van Valderen, R.D. (author), van der Zwaan, R. (author), van de Wijgaart, R. (author), Lin, Y. (author), Abbas, B.A. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)

The tremendous progress in sequencing technologies has made DNA sequencing routine for microbiome studies. Additionally, advances in mass spectrometric techniques have extended conventional proteomics into the field of microbial ecology. However, systematic studies that provide a better understanding of the complementary nature of these ...

journal article 2023

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Insight on how biopolymers recovered from aerobic granular wastewater sludge can reduce the flammability of synthetic polymers

Kim, Nam Kyeun (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Eco-friendly flame retardants are greatly required to meet the expectations of low-toxicity, environmental compatibility and sustainability. Extracellular polymeric substances (EPS), the biopolymers recovered from excess granular wastewater sludge, have been successfully incorporated into poly(vinyl alcohol) (PVA) by a solution casting method...

journal article 2022

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Sulfated glycosaminoglycan-like polymers are present in an acidophilic biofilm from a sulfidic cave

de Bruin, S. (author), Vasquez Cardenas, D. (author), Sarbu, S. M. (author), Meysman, F.J.R. (author), Sousa, D. Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Sulfated glycosaminoglycans (sGAG) are negatively charged extracellular polymeric substances that occur in biofilms from various environments. Yet, it remains unclear whether these polymers are acquired from the external environment or produced by microbes in the biofilm. To resolve this, we analyzed the presence of sGAGs in samples of an...

journal article 2022

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A review on recovery of extracellular biopolymers from flocculent and granular activated sludges: Cognition, key influencing factors, applications, and challenges

Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)

A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...

review 2022

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Enrichment and application of extracellular nonulosonic acids containing polymers of Accumulibacter

Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...

journal article 2022

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Catabolism of sialic acids in an environmental microbial community

Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...

journal article 2022

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Production of nonulosonic acids in the extracellular polymeric substances of “Candidatus Accumulibacter phosphatis”

Tomas Martinez, S. (author), Kleikamp, H.B.C. (author), Neu, Thomas R. (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular...

journal article 2021

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Flame retardant property of flax fabrics coated by extracellular polymeric substances recovered from both activated sludge and aerobic granular sludge

Kim, Nam Kyeun (author), Mao, Ningtao (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

In this research, extracellular polymeric substances (EPS), such as EPS_flocs and EPS_granules, were successfully extracted from activated and aerobic granular sludge, respectively, and tested as bio-based flame retardant materials. Flax fabric was coated by the biopolymeric substances and its flammability was evaluated...

journal article 2020

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Impact of metal ions on structural EPS hydrogels from aerobic granular sludge

Felz, S. (author), Kleikamp, H.B.C. (author), Zlopasa, J. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)

Structural extracellular polymeric substances (structural EPS) can form stable hydrogels and are considered to be responsible for the stability of biofilms and aerobic granular sludge. Structural EPS were extracted from aerobic granular sludge and characterized for their gel-forming capacity with different alkaline earth and transition metal...

journal article 2020

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Anticipating Xenogenic Pollution at the Source: Impact of Sterilizations on DNA Release From Microbial Cultures

Calderon Franco, D. (author), Lin, Qingnan (author), van Loosdrecht, Mark C.M. (author), Abbas, B.A. (author), Weissbrodt, D.G. (author)

The dissemination of DNA and xenogenic elements across waterways is under scientific and public spotlight due to new gene-editing tools, such as do-it-yourself (DIY) CRISPR-Cas kits deployable at kitchen table. Over decades, prevention of spread of genetically modified organisms (GMOs), antimicrobial resistances (AMR), and pathogens from...

journal article 2020

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Tackling the chemical diversity of microbial nonulosonic acids-a universal large-scale survey approach

Kleikamp, H.B.C. (author), Lin, Y. (author), McMillan, D.G.G. (author), Geelhoed, Jeanine S. (author), Naus-Wiezer, Suzanne N.H. (author), Van Baarlen, Peter (author), Saha, Chinmoy (author), Louwen, Rogier (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)

Nonulosonic acids, commonly referred to as sialic acids, are a highly important group of nine-carbon sugars common to all domains of life. They all share biosynthetic and structural features, but otherwise display a remarkable chemical diversity. In humans, sialic acids cover all cells which makes them important for processes such as cellular...

review 2020