Pancharatnam–Berry phase shaping for control of the transverse enhancement of focusing

We show that elongating a tightly focused field in the direction perpendicular to the optical axis is possible. We demonstrate our approach by specially shaping the Pancharatnam–Berry (PB) phase. Moreover, the analytical formulae required to calculate the strength vectors and energy flux of the three-dimensional electromagnetic fields near...

Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity

The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (gRNA) and the targeted DNA, yet the sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore the sequence determinants governing CRISPR/Cas9 specificity, here we describe a dual-target system to...