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Calderon Franco, D. (author), Sarelse, R.G. (author), Christou, S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)In the One Health context, wastewater treatment plants (WWTPs) are central to safeguarding water resources. Nonetheless, many questions remain about their effectiveness in preventing antimicrobial resistance (AMR) dissemination. Most surveillance studies monitor the levels and removal of selected antibiotic resistance genes (ARGs) and mobile...journal article 2022
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Calderon Franco, D. (author), Orschler, Laura (author), Lackner, Susanne (author), Agrawal, Shelesh (author), Weissbrodt, D.G. (author)The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemia has been one of the most difficult challenges humankind has recently faced. Wastewater-based epidemiology has emerged as a tool for surveillance and mitigation of potential viral outbreaks, circumventing biases introduced by clinical patient testing. Due to the situation...journal article 2022
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Calderon Franco, D. (author), Apoorva, Seeram (author), Medema, G.J. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Wastewater treatment is challenged by the continuous emergence of chemical and biological contaminants. Disinfection, advanced oxidation, and activated carbon technologies are accessible in high-income countries to suppress them. Low-cost, easily implementable, and scalable solutions are needed for sanitation across regions. We studied the...journal article 2021
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Cerruti, M. (author), Kim, J.H. (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Purple bacteria (PPB), anoxygenic photoorganoheterotrophic organisms with a hyper-versatile metabolism and high biomass yields over substrate, are promising candidates for the recovery of nutrient resources from wastewater. Infrared light is a pivotal parameter to control and design PPB-based resource recovery. However, the effects of light...journal article 2022
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Calderon Franco, D. (author), Corbera Rubio, F. (author), Cuesta Sanz, M. (author), Pieterse, Brent (author), de Ridder, David (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author), Laureni, M. (author), Weissbrodt, D.G. (author)Drinking water treatment plants (DWTPs) are designed to remove physical, chemical, and biological contaminants. However, until recently, the role of DWTPs in minimizing the cycling of antibiotic resistance determinants has got limited attention. In particular, the risk of selecting antibiotic-resistant bacteria (ARB) is largely overlooked in...journal article 2023
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Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)The free-floating extracellular DNA (exDNA) fraction of microbial ecosystems harbors antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs). Natural transformation of these xenogenetic elements can generate microbial cells resistant to one or more antibiotics. Isolating and obtaining high yield exDNA is challenging due to its low...journal article 2020
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Teixeira, A. Margarida (author), Vaz-Moreira, Ivone (author), Calderon Franco, D. (author), Weissbrodt, D.G. (author), Purkrtova, Sabina (author), Gajdos, Stanislav (author), Dottorini, Giulia (author), Nielsen, Per H. (author), Khalifa, Leron (author), Cytryn, Eddie (author), Bartacek, Jan (author), Manaia, Célia M. (author)Urban wastewater treatment plants (UWTPs) are essential for reducing the pollutants load and protecting water bodies. However, wastewater catchment areas and UWTPs emit continuously antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs), with recognized impacts on the downstream environments. Recently, the European Commission...journal article 2023
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Piaggio, A.L. (author), Mittapalli, Srilekha (author), Calderon Franco, D. (author), Weissbrodt, D.G. (author), van Lier, J.B. (author), de Kreuk, M.K. (author), Lindeboom, R.E.F. (author)This study investigates the effects, conversions, and resistance induction, following the addition of 150 μg·L<sup>−1 </sup>of two antibiotics, sulfamethoxazole (SMX) and trimethoprim (TMP), in a laboratory-scale micro-aerated anaerobic membrane bioreactor (MA-AnMBR). TMP and SMX were removed at 97 and 86%, indicating that micro-aeration did not...journal article 2023
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Giulianetti de Almeida, M.P. (author), Mockaitis, Gustavo (author), Weissbrodt, D.G. (author)Milk discovery and processing enabled human settling and thriving in various settings. The discovery of cheese led to the production of whey as dairy by-product. Although it can find application in food, beverages, personal care products, pharmaceuticals and medical treatment, cheese whey is a massive dairying residue world-wide (154 Mm3·y-1)...journal article 2021
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Kouba, V. (author), D, Vejmelkova (author), E, Zwolsman (author), K, Hurkova (author), K, Navratilova (author), M, Laureni (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Anammox bacteria enable efficient removal of nitrogen from sewage in processes involving partial nitritation and anammox (PN/A) or nitrification, partial denitrification, and anammox (N-PdN/A). In mild climates, anammox bacteria must be adapted to ≤15 °C, typically by gradual temperature decrease; however, this takes months or years. To...journal article 2022
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Alloul, A. (author), Cerruti, M. (author), Adamczyk, Damian (author), Weissbrodt, D.G. (author), Vlaeminck, Siegfried E. (author)Purple non-sulfur bacteria (PNSB) show potential for microbial protein production on wastewater as animal feed. They offer good selectivity (i.e., low microbial diversity and high abundance of one species) when grown anaerobically in the light. However, the cost of closed anaerobic photobioreactors is prohibitive for protein production....journal article 2021
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Pallares Vega, R. (author), Macedo, Gonçalo (author), Brouwer, Michael S.M. (author), Hernandez Leal, Lucia (author), van der Maas, Peter (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Heederik, Dick (author), Mevius, Dik (author), Schmitt, Heike (author)Plasmid-mediated dissemination of antibiotic resistance among fecal Enterobacteriaceae in natural ecosystems may contribute to the persistence of antibiotic resistance genes in anthropogenically impacted environments. Plasmid transfer frequencies measured under laboratory conditions might lead to overestimation of plasmid transfer potential...journal article 2021
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), van Niftrik, Laura (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The adaptation of bacteria involved in anaerobic ammonium oxidation (anammox) to low temperatures will enable more efficient removal of nitrogen from sewage across seasons. At lower temperatures, bacteria typically tune the synthesis of their membrane lipids to promote membrane fluidity. However, such adaptation of anammox bacteria lipids,...journal article 2022
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Alloul, A. (author), Van Kampen, W. (author), Cerruti, M. (author), Wittouck, S. (author), Pabst, Martin (author), Weissbrodt, D.G. (author)Purple non-sulphur bacteria (PNSB) are an emerging group of microbes attractive for applied microbiology applications such as wastewater treatment, plant biostimulants, microbial protein, polyhydroxyalkanoates and H<sub>2</sub> production. These photoorganoheterotrophic microbes have the unique ability to grow selectively on organic carbon in...journal article 2022
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Seviour, R (author), Tooker, Nick (author), Vainio, Pirjo (author), Dueholm, Morten Kam Dahl (author), Nierychlo, Marta (author), Andersen, Kasper Skytte (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Rossetti, S (author)Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’...journal article 2022
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Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...journal article 2022
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Cerruti, M. (author), Crosset-Perrotin, G. (author), Ananth, Mythili (author), Rombouts, Julius Laurens (author), Weissbrodt, D.G. (author)Fermentative chemoorganoheterotrophic bacteria (FCB) and purple photoorganoheterotrophic bacteria (PPB) are two interesting microbial guilds to process carbohydrate-rich wastewaters. Their metabolic interactions have been studied in pure cultures or co-cultures, but little is known about mixed cultures. We studied the effect of reactor...journal article 2023
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Fumasoli, Alexandra (author), Bürgmann, Helmut (author), Weissbrodt, D.G. (author), Wells, G.N. (author), Beck, Karin (author), Mohn, Joachim (author), Morgenroth, Eberhard (author), Udert, Kai M. (author)Ammonia oxidation decreases the pH in wastewaters where alkalinity is limited relative to total ammonia. The activity of ammonia oxidizing bacteria (AOB), however, typically decreases with pH and often ceases completely in slightly acidic wastewaters. Nevertheless, nitrification at low pH has been reported in reactors treating human urine,...journal article 2017
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Laureni, M. (author), Weissbrodt, D.G. (author), Villez, Kris (author), Robin, Orlane (author), de Jonge, Nadieh (author), Rosenthal, Alex (author), Wells, George (author), Nielsen, Jeppe Lund (author), Morgenroth, Eberhard (author), Joss, Adriano (author)The control of nitrite-oxidizing bacteria (NOB) challenges the implementation of partial nitritation and anammox (PN/A) processes under mainstream conditions. The aim of the present study was to understand how operating conditions impact microbial competition and the control of NOB in hybrid PN/A systems, where biofilm and flocs coexist. A...journal article 2019
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Policastro, G. (author), Ebrahimi, S. (author), Weissbrodt, D.G. (author), Fabbricino, Massimiliano (author), van Loosdrecht, Mark C.M. (author)A dual-growth-limited continuous operated bioreactor (chemostat) was used to enhance lipid accumulation in an enrichment culture of microalgae. The light intensity and nitrogen concentration where both limiting factors resulting in high lipid accumulation in the mixed culture. Both conditions of light and nitrogen excess and deficiency were...journal article 2024