- document
-
Ziliani, Agustina (author), Bovio-Winkler, Patricia (author), Cabezas, Angela (author), Etchebehere, Claudia (author), Garcia, Hector A. (author), López-Vázquez, Carlos M. (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Rubio-Rincón, Francisco J. (author)Ca. Accumulibacter was the predominant microorganism (relative FISH bio-abundance of 67 ± 5%) in a lab-scale sequential batch reactor that accomplished enhanced biological phosphorus removal (EBPR) while using glucose and acetate as the carbon sources (1:1 COD-based ratio). Both organic compounds were completely anaerobically consumed. The...journal article 2023
- document
-
Hu, Zhi Yi (author), Lin, Yi Peng (author), Wang, Qing Ting (author), Zhang, Yi Xin (author), Tang, Jie (author), Wang, Shuai (author), van Loosdrecht, Mark C.M. (author), Zeng, Raymond Jianxiong (author), Zhang, Fang (author)By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and...journal article 2023
- document
-
Zhang, Zi (author), Sato, Yugo (author), Dai, Ji (author), Chui, Ho kwong (author), Daigger, Glen (author), van Loosdrecht, Mark C.M. (author), Chen, Guanghao (author)Exploring alternative water sources and improving the efficiency of energy uses are crucial approaches to strengthening the water-energy securities and achieving carbon mitigations in sub(tropical) coastal cities. Seawater use for toilet flushing and district cooling systems is reportedly practical for achieving multiaspect benefits in Hong...journal article 2023
- document
-
Chen, L.M. (author), Keisham, Sunanda (author), Tateno, Hiroaki (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Bacteria can synthesize a diverse array of glycans, being found attached to proteins and lipids or as loosely associated polysaccharides to the cells. The major challenge in glycan analysis in environmental samples lies in developing high-throughput and comprehensive characterization methodologies to elucidate the structure and monitor the...journal article 2023
- document
-
Li, Zejiao (author), Wang, Jixiang (author), Liu, Jialin (author), Chen, Xingyu (author), Lei, Zhongfang (author), Yuan, Tian (author), Lee, Duu Jong (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)Reducing CO<sub>2</sub> emission and energy consumption is crucial for the sustainable management of wastewater treatment plants (WWTPs). In this study, an algal-bacterial aerobic granular sludge (AGS) system was developed for efficient carbon (C) assimilation and nitrogen (N)/phosphorus (P) removal without the need for mechanical aeration....journal article 2023
- document
-
Li, Ji (author), Hao, Xiaodi (author), Shen, Zhan (author), Wu, Yuanyuan (author), van Loosdrecht, Mark C.M. (author)A high moisture content of waste activated sludge (WAS) associated with a low calorific value needs to be deeply dried towards self-supporting incineration. On the other hand, thermal energy with low temperature exchanged from treated effluent has great potential for drying sludge. Unfortunately, low-temperature drying of sludge seems to be...journal article 2023
- document
-
Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)The transformation of environmental microorganisms by extracellular DNA is an overlooked mechanism of horizontal gene transfer and evolution. It initiates the acquisition of exogenous genes and propagates antimicrobial resistance alongside vertical and conjugative transfers. We combined mixed-culture biotechnology and Hi-C sequencing to...journal article 2023
- document
-
Sorokin, Dimitry Y. (author), Tikhonova, Tamara V. (author), Koch, Hanna (author), van den Berg, E.M. (author), Hinderks, Renske S. (author), Pabst, Martin (author), Kuenen, J.G. (author), van Loosdrecht, Mark C.M. (author), Lücker, Sebastian (author)Dissimilatory nitrate reduction to ammonia (DNRA) is a common biochemical process in the nitrogen cycle in natural and man-made habitats, but its significance in wastewater treatment plants is not well understood. Several ammonifying Trichlorobacter strains (former Geobacter) were previously enriched from activated sludge in nitrate-limited...journal article 2023
- document
-
Ali, Muhammad (author), Singh, Yogesh (author), Fortunato, Luca (author), Rehman, Zahid Ur (author), Manjunath, Sarvajith (author), Vrouwenvelder, J.S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)This study describes a novel integration of aerobic granular sludge (AGS) with a gravity-driven membrane (GDM) system at a pilot scale with a treatment capacity of approximately 150 L per day to treat raw domestic wastewater. The treatment performance and energy consumption of the AGS-GDM system were compared to the neighboring full-scale...journal article 2023
- document
-
Yin, Xiaole (author), Chen, Xi (author), Jiang, Xiao Tao (author), Yang, Ying (author), Li, Bing (author), Shum, Marcus Ho Hin (author), Schmitt, H. (author), Cha, Chang Jun (author), van Loosdrecht, Mark C.M. (author)Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health"framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and...journal article 2023
- document
-
Moradi, N. (author), Vazquez, Carlos Lopez (author), Hernandez, Hector Garcia (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Rincón, Francisco Rubio (author)Digestate is a rich source of nutrients that can be applied in agricultural fields as fertilizer or irrigation water. However, most of the research about application of digestate have focused on its agronomic properties and neglected the potential harm of the presence of contaminants of emerging concern (CECs). Aadvanced oxidation processes ...journal article 2023
- document
-
Li, Xuan (author), Liu, Huan (author), Gao, Li (author), Sherchan, Samendra P. (author), Zhou, Ting (author), Khan, Stuart J. (author), van Loosdrecht, Mark C.M. (author), Wang, Qilin (author)Although the coronavirus disease (COVID-19) emergency status is easing, the COVID-19 pandemic continues to affect healthcare systems globally. It is crucial to have a reliable and population-wide prediction tool for estimating COVID-19-induced hospital admissions. We evaluated the feasibility of using wastewater-based epidemiology (WBE) to...journal article 2023
- document
-
Pei, R. (author), Vicente-Venegas, Gerard (author), Tomaszewska-Porada, Agnieszka (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge...journal article 2023
- document
-
de Jong, S.I. (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author), McMillan, D.G.G. (author)Proteomics has greatly advanced the understanding of the cellular biochemistry of microorganisms. The thermoalkaliphile Caldalkalibacillus thermarum TA2.A1 is an organism of interest for studies into how alkaliphiles adapt to their extreme lifestyles, as it can grow from pH 7.5 to pH 11. Within most classes of microbes, the membrane-bound...journal article 2023
- document
-
Chen, L.M. (author), de Bruin, S. (author), Pronk, M. (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Anaerobic and aerobic granular sludge processes are widely applied in wastewater treatment. In these systems, microorganisms grow in dense aggregates due to the production of extracellular polymeric substances (EPS). This study investigates the sialylation and sulfation of anionic glyconconjugates in anaerobic and aerobic granular sludges...journal article 2023
- document
-
Tabakovic, A. (author), Lemmens, Jeremy (author), Tamis, J. (author), van Vliet, Dave (author), Nahar, Sayeda (author), Suitela, Willem (author), van Loosdrecht, Mark C.M. (author), Leegwater, G.A. (author)Bitumen is a key constitutive material in asphalt pavements. It binds together the rock scaffolding of a pavement. Bitumen provides asphalt pavement with flexibility and enables it to respond to traffic loading and return to its original condition after the loading, i.e. bitumen restores/repairs the damage. In Porous Asphalt (PA) or...journal article 2023
- document
-
Olavarria, Karel (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages, such as increased yields and reduced energy dissipation. However, lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic equilibrium. While stoichiometric analyses and...journal article 2023
- document
-
Ahmadi, Navid (author), Abbasi, Mona (author), Torabian, Ali (author), van Loosdrecht, Mark C.M. (author), Ducoste, Joel (author)We investigated the transformation of four pharmaceuticals (Diclofenac, Naproxen, Ibuprofen and Carbamazepine) in a moving bed biofilm reactor subjected to different COD/N ratios in four experimental phases. The shift from medium to high range COD/N ratio (i.e., 5:1 to 100:1) intensified the competition between heterotrophs and nitrifying...journal article 2023
- document
-
Kim, Nam Kyeun (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)In the present research, a bio-based flame retardant (FR) was prepared using a biopolymer derived from wastewater sludge to improve the fire performance of polypropylene (PP). Extracellular polymeric substances (EPS), which were extracted from wastewater aerobic granular sludge, were absorbed into cellulose-based fibres, such as flax and...journal article 2023
- document
-
Páez Watson, T.W. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The understanding of microbial communities and the biological regulation of its members is crucial for implementation of novel technologies using microbial ecology. One poorly understood metabolic principle of microbial communities is resource allocation and biosynthesis. Resource allocation theory in polyphosphate accumulating organisms ...journal article 2023