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Haaksman, V.A. (author), Schouteren, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)
There is a growing interest to implement aerobic granular sludge (AGS) in existing conventional activated sludge (CAS) systems with a continuous flow-through configuration. The mode of anaerobic contact of raw sewage with the sludge is an important aspect in the adaptation of CAS systems to accommodate AGS. It remains unclear how the...
journal article 2023
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Corbera Rubio, F. (author), Laureni, M. (author), Koudijs, N. (author), Müller, S. (author), van Alen, Theo (author), Schoonenberg, Frank (author), Lücker, Sebastian (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author)
Rapid sand filters (RSF) are an established and widely applied technology for groundwater treatment. Yet, the underlying interwoven biological and physical-chemical reactions controlling the sequential removal of iron, ammonia and manganese remain poorly understood. To resolve the contribution and interactions between the individual reactions...
journal article 2023
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Bahgat, T.M.S.M. (author), Wilfert, P.K. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author)
The study evaluated the combined phosphorus, nitrogen, methane, and extracellular polymeric substances (EPS) recovery from aerobic granular sludge (AGS) wastewater treatment plants. About 30% of sludge organics are recovered as EPS and 25–30% as methane (≈260 ml methane/g VS) by integrating alkaline anaerobic digestion (AD). It was shown that...
journal article 2023
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Chen, Le Min (author), Erol, Özlem (author), Choi, Young Hae (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)
Currently, there is a growing interest in transforming wastewater treatment plants (WWTPs) into resource recovery plants. Microorganisms in aerobic granular sludge produce extracellular polymeric substances (EPS), which are considered sustainable resources to be extracted and can be used in diverse applications. Exploring applications in other...
journal article 2024
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Rahimi, Zahra (author), Zinatizadeh, Ali Akbar (author), Zinadini, Sirus (author), van Loosdrecht, Mark C.M. (author), JBatstone, Damien (author)
In this work, a one-stage dual internal circulation airlift anaerobic/anoxic/aerobic (DCAL-A2O) bioreactor was continuously operated for concurrent removal of nutrients and organics from milk processing wastewater (MPW). Special configuration of the airlift A2O bioreactor created possibility of the formation of desired anaerobic, anoxic and...
journal article 2024
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Corbera Rubio, F. (author), Stouten, G.R. (author), Bruins, Jantinus (author), Dost, Simon F. (author), Merkel, Alexander Y. (author), Müller, S. (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author), Laureni, M. (author)
Nitrate leaching from agricultural soils is increasingly found in groundwater, a primary source of drinking water worldwide. This nitrate influx can potentially stimulate the biological oxidation of iron in anoxic groundwater reservoirs. Nitrate-dependent iron-oxidizing (NDFO) bacteria have been extensively studied in laboratory settings, yet...
journal article 2024
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Martinez Quintela, M. (author), Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Suárez, S. (author), Omil, F. (author), Weissbrodt, D.G. (author)
In activated sludge, the antibiotic resistance genes (ARGs) can be present either in the intracellular (iDNA) or extracellular DNA fraction (exDNA). Recent advances in the exDNA extraction methodology allow a better profiling of the pool of ARGs. However, little is known about how stress conditions modify the distribution of ARGs between both...
journal article 2024
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Abkar, Leili (author), Aghili Mehrizi, Amirreza (author), Jafari, Morez (author), Beck, Sara E. (author), Ghassemi, Abbas (author), van Loosdrecht, Mark C.M. (author)
Reverse osmosis (RO) systems offer a viable solution for treating brackish water (BW), a common but underutilized water resource. However, the energy-intensive nature of brackish water reverse osmosis (BWRO) systems poses affordability challenges to water supply, necessitating a focus on minimizing their energy consumption to support SDG6's...
journal article 2024
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Prot, T.J.F. (author), Nguyen, V. H. (author), Wilfert, P.K. (author), Dugulan, A.I. (author), Goubitz, K. (author), de Ridder, D.J. (author), Korving, L. (author), Rem, P.C. (author), Bouderbala, A. (author), Witkamp, G.J. (author), van Loosdrecht, Mark C.M. (author)
To prevent eutrophication of surface water, phosphate needs to be removed from sewage. Iron (Fe) dosing is commonly used to achieve this goal either as the main strategy or in support of biological removal. Vivianite (Fe(II) <sub>3</sub> (PO <sub>4</sub> ) <sub>2</sub> * 8H <sub>2</sub> O) plays a crucial role in capturing the phosphate, and...
journal article 2019
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Culcasi, A. (author), Ktori, R. (author), Pellegrino, A. (author), Rodriguez Pascual, M. (author), van Loosdrecht, Mark C.M. (author), Tamburini, A. (author), Cipollina, A. (author), Xevgenos, Dimitris (author), Micale, G. (author)
European policy encourages the adoption of sustainable systems that promote the efficient use and recovery of minerals and chemicals. In this respect, desalination brines do contain a dramatic amount of valuable minerals and can be valorized through appropriate treatments rather than releasing them into the environment. This paper proposes an...
journal article 2022
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Keuten, M.G.A. (author), Peters, M.C.F.M. (author), van Dijk, J.C. (author), van Loosdrecht, Mark C.M. (author), Rietveld, L.C. (author)
other 2017
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Verhagen, K.J.A. (author), Gabriel Guedes da Silva, L. (author), van de Wijgaart, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)
Natural habitats of microorganisms are dynamic environments with non-continuous supply of carbon and energy sources, in which intermediate storage of substrates can increase competitiveness. Plasticicumulans acidivorans are polyhydroxybutyrate (PHB) accumulating bacteria enriched from activated sludge using carbon feast-famine cycles as...
poster 2017
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Kouba, V. (author), Bachmannová, Ch (author), Podzimek, T. (author), Lipovová, P. (author), van Loosdrecht, Mark C.M. (author)
The adaptation of bacteria involved in the anaerobic ammonium oxidation (anammox) to low temperatures in the mainstream of WWTP will unlock substantial treatment savings. However, their adaptation mechanisms have begun to be revealed only very recently. This study reviewed the state-of-the-art knowledge on these mechanisms from -omics studies...
review 2022
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Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)
A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...
review 2022
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Suresh Kumar, P. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)
Eutrophication and the resulting formation of harmful algal blooms (HAB) causes huge economic and environmental damages. Phosphorus (P) from sewage effluent and agricultural run-off has been identified as a major cause for eutrophication. Phosphorous concentrations greater than 100 μg P/L are usually considered high enough to cause...
review 2019
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Ruiz Hadad, L. (author), Ali, Muhammad (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)
Currently, the most cost-effective and efficient method for phosphorus (P) removal from wastewater is enhanced biological P removal (EPBR) via polyphosphate-accumulating organisms (PAOs). This study integrates a literature review with genomic analysis to uncover the phylogenetic and metabolic diversity of the relevant PAOs for wastewater...
review 2024
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Soler Jofra, A. (author), Pérez, Julio (author), van Loosdrecht, Mark C.M. (author)
Aerobic ammonium oxidizing bacteria were first isolated more than 100 years ago and hydroxylamine is known to be an intermediate. The enzymatic steps involving hydroxylamine conversion to nitrite are still under discussion. For a long time it was assumed that hydroxylamine was directly converted to nitrite by a hydroxylamine oxidoreductase....
review 2021
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Estevez Alonso, A. (author), Pei, R. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)
Conversion of organic waste and wastewater to polyhydroxyalkanoates (PHAs) offers a potential to recover valuable resources from organic waste. Microbial community-based PHA production systems have been successfully applied in the last decade at lab- and pilot-scales, with a total of 19 pilot installations reported in the scientific...
review 2021
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Kehrein, P.A. (author), van Loosdrecht, Mark C.M. (author), Osseweijer, P. (author), Garfí, Marianna (author), Dewulf, Jo (author), Posada Duque, J.A. (author)
In recent decades, academia has elaborated a wide range of technological solutions to recover water, energy, fertiliser and other products from municipal wastewater treatment plants. Drivers for this work range from low resource recovery potential and cost effectiveness, to the high energy demands and large environmental footprints of current...
review 2020
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Kleikamp, H.B.C. (author), Lin, Y. (author), McMillan, D.G.G. (author), Geelhoed, Jeanine S. (author), Naus-Wiezer, Suzanne N.H. (author), Van Baarlen, Peter (author), Saha, Chinmoy (author), Louwen, Rogier (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)
Nonulosonic acids, commonly referred to as sialic acids, are a highly important group of nine-carbon sugars common to all domains of life. They all share biosynthetic and structural features, but otherwise display a remarkable chemical diversity. In humans, sialic acids cover all cells which makes them important for processes such as cellular...
review 2020
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