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van den Berg, L. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Granular sludge processes are frequently used in domestic and industrial wastewater treatment. The granule buoyant density and biomass density are important parameters for the design and operation of granular sludge reactors. Different methods to measure the granule density include the pycnometer method, the Percoll density gradient method,...journal article 2022
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Kim, Nam Kyeun (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Eco-friendly flame retardants are greatly required to meet the expectations of low-toxicity, environmental compatibility and sustainability. Extracellular polymeric substances (EPS), the biopolymers recovered from excess granular wastewater sludge, have been successfully incorporated into poly(vinyl alcohol) (PVA) by a solution casting method...journal article 2022
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Li, Ji (author), Hao, Xiaodi (author), Gan, W. (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Alginate like extracellular polymers (ALE) recovered from flocculent sludge has been identified as a kind of highly valuable biomaterials. However, the extraction protocols limit the production of biopolymers as ALE extracted from flocculent sludge is at a lower level, around 90–190 mg/g VSS. Under this circumstance, the eco-friendly and...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), van Niftrik, Laura (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The adaptation of bacteria involved in anaerobic ammonium oxidation (anammox) to low temperatures will enable more efficient removal of nitrogen from sewage across seasons. At lower temperatures, bacteria typically tune the synthesis of their membrane lipids to promote membrane fluidity. However, such adaptation of anammox bacteria lipids,...journal article 2022
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van Dijk, E.J.H. (author), Haaksman, V.A. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)In this study a mathematical framework was developed to describe aerobic granulation based on 6 main mechanisms: microbial selection, selective wasting, maximizing transport of substrate into the biofilm, selective feeding, substrate type and breakage. A numerical model was developed using four main components; a 1D convection/dispersion...journal article 2022
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Li, Ji (author), Hao, Xiaodi (author), Gan, Wei (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Alginate like extracellular polymers (ALE) recovered from excess sludge have been evaluated as an eco-friendly, cost effective and sustainable alternative to highly valued materials. However, the ALE extraction from flocculent sludge ranges normally from 90 to 190 mg/g VSS, which is only equivalent to the lowest edge of the ALE production...journal article 2022
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de Bruin, S. (author), Vasquez Cardenas, D. (author), Sarbu, S. M. (author), Meysman, F.J.R. (author), Sousa, D. Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sulfated glycosaminoglycans (sGAG) are negatively charged extracellular polymeric substances that occur in biofilms from various environments. Yet, it remains unclear whether these polymers are acquired from the external environment or produced by microbes in the biofilm. To resolve this, we analyzed the presence of sGAGs in samples of an...journal article 2022
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Seviour, R (author), Tooker, Nick (author), Vainio, Pirjo (author), Dueholm, Morten Kam Dahl (author), Nierychlo, Marta (author), Andersen, Kasper Skytte (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Rossetti, S (author)Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’...journal article 2022
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...journal article 2022
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Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...journal article 2022
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Sagir Kurt, Ece (author), Atli, Ezgi (author), Basa, Safak (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author)Aerobic granules contain microorganisms that are responsible for carbon, nitrogen, and phosphorus removal in aerobic granular sludge (AGS) process in which aerobic/anoxic/anaerobic layers (from surface to core) occur in a single granule. Optimizing the aerobic granular sludge (AGS) process for granulation and efficient nutrient removal can be...journal article 2022
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Olavarria, Karel (author), Pijman, Yared O. (author), Cabrera, Ricardo (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The coupling of PHB generation with NADH reoxidation is required to generate PHB as a fermentation product. A fundamental trait to accomplish this feature is to express a functional NADH-preferring acetoacetyl-CoA reductase, engaged in PHB accumulation. One way to obtain such a reductase is by engineering the cofactor preference of the...journal article 2022
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Pei, R. (author), Vicente-Venegas, Gerard (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoate accumulation experiments at pilot scale were performed with fullscale municipal waste activated sludge. Development of biomass PHA content was quantified by thermogravimetric analysis. Over 48 h the biomass reached up to 0.49 ± 0.03 gPHA/gVSS (n=4). Samples were processed in parallel to characterise the distribution of...journal article 2022
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Hao, Xiaodi (author), Wang, Xiangyang (author), Shi, Chen (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Sludge incineration and seawater desalination are two approaches that can be used in the disposal of waste activated sludge (WAS) and for obtaining fresh water. As resource recovery from wastewater treatment and water purification is a topic of particular interest in these times, “water mining” has become a focus of research, with phosphate/P...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), Vodičková, Patricie (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The application of partial nitritation-anammox (PN/A) under mainstream conditions can enable substantial cost savings at wastewater treatment plants (WWTPs), but how process conditions and cell physiology affect anammox performance at psychrophilic temperatures below 15 °C remains poorly understood. We tested 14 anammox communities, including...journal article 2022
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Ekholm, Jennifer (author), Persson, Frank (author), de Blois, Mark (author), Modin, Oskar (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Suarez, Carolina (author), Gustavsson, David J.I. (author), Wilén, Britt Marie (author)Aerobic granular sludge (AGS) plants have gained growing interest and application due to their low energy demand, small footprint, and low operational costs. However, the fulfilment of strict discharge limits for nitrogen and phosphorus, vast seasonal temperature variations, and large peaks in influent flows may pose challenges to the...journal article 2022
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Prot, T.J.F. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)In a wastewater treatment plant (WWTP), several sludge streams exist and the composition of their liquid phase varies with time and place. For evaluating the potential for formation of precipitates and equilibria for weak acids/bases, the ionic strength and chemical composition need to be known. This information is often not available in...journal article 2022
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Hao, Xiaodi (author), Wu, Daoqi (author), Li, Ji (author), Liu, Ranbin (author), van Loosdrecht, Mark C.M. (author)Entropy is a concept defined by the second law of thermodynamics. Applying this concept to the world we live in, entropy production must be minimized and negentropy (negative entropy production) should be accelerated, in order to produce a healthy and stable ecological system. The present wastewater treatment, however, contributes to entropy...journal article 2022