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Soler Jofra, A. (author), Schmidtchen, Lisbeth (author), Olmo, Lluc (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)Hydroxylamine is a nitrogen intermediate of ammonium oxidizing bacteria (AOB) that can transiently accumulate during nitrification. The impact of hydroxylamine on aerobic ammonium oxidations is still obscure. In the present study the short and long term impact of hydroxylamine on partial nitritation granular sludge was investigated. Dissolved...journal article 2022
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Wijdeveld, W. K. (author), Prot, T.J.F. (author), Sudintas, G. (author), Kuntke, P. (author), Korving, L. (author), van Loosdrecht, Mark C.M. (author)Phosphorus (P) is an essential resource for food production and chemical industry. Phosphorus use has to become more sustainable and should include phosphorus recycling from secondary sources. About 20% of P ends up in sewage sludge, making this a substantial secondary P source. There is currently a technological gap to recover P from sludge...journal article 2022
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Kouba, V. (author), Bachmannová, Ch (author), Podzimek, T. (author), Lipovová, P. (author), van Loosdrecht, Mark C.M. (author)The adaptation of bacteria involved in the anaerobic ammonium oxidation (anammox) to low temperatures in the mainstream of WWTP will unlock substantial treatment savings. However, their adaptation mechanisms have begun to be revealed only very recently. This study reviewed the state-of-the-art knowledge on these mechanisms from -omics studies...review 2022
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Kouba, V. (author), D, Vejmelkova (author), E, Zwolsman (author), K, Hurkova (author), K, Navratilova (author), M, Laureni (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Anammox bacteria enable efficient removal of nitrogen from sewage in processes involving partial nitritation and anammox (PN/A) or nitrification, partial denitrification, and anammox (N-PdN/A). In mild climates, anammox bacteria must be adapted to ≤15 °C, typically by gradual temperature decrease; however, this takes months or years. To...journal article 2022
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van den Berg, L. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Granular sludge processes are frequently used in domestic and industrial wastewater treatment. The granule buoyant density and biomass density are important parameters for the design and operation of granular sludge reactors. Different methods to measure the granule density include the pycnometer method, the Percoll density gradient method,...journal article 2022
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Kim, Nam Kyeun (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Eco-friendly flame retardants are greatly required to meet the expectations of low-toxicity, environmental compatibility and sustainability. Extracellular polymeric substances (EPS), the biopolymers recovered from excess granular wastewater sludge, have been successfully incorporated into poly(vinyl alcohol) (PVA) by a solution casting method...journal article 2022
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Li, Ji (author), Hao, Xiaodi (author), Gan, W. (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Alginate like extracellular polymers (ALE) recovered from flocculent sludge has been identified as a kind of highly valuable biomaterials. However, the extraction protocols limit the production of biopolymers as ALE extracted from flocculent sludge is at a lower level, around 90–190 mg/g VSS. Under this circumstance, the eco-friendly and...journal article 2022
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Kouba, Vojtěch (author), Hůrková, Kamila (author), Navrátilová, Klára (author), Kok, Dana (author), Benáková, Andrea (author), Laureni, M. (author), van Niftrik, Laura (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)The adaptation of bacteria involved in anaerobic ammonium oxidation (anammox) to low temperatures will enable more efficient removal of nitrogen from sewage across seasons. At lower temperatures, bacteria typically tune the synthesis of their membrane lipids to promote membrane fluidity. However, such adaptation of anammox bacteria lipids,...journal article 2022
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van Dijk, E.J.H. (author), Haaksman, V.A. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)In this study a mathematical framework was developed to describe aerobic granulation based on 6 main mechanisms: microbial selection, selective wasting, maximizing transport of substrate into the biofilm, selective feeding, substrate type and breakage. A numerical model was developed using four main components; a 1D convection/dispersion...journal article 2022
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Li, Ji (author), Hao, Xiaodi (author), Gan, Wei (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Alginate like extracellular polymers (ALE) recovered from excess sludge have been evaluated as an eco-friendly, cost effective and sustainable alternative to highly valued materials. However, the ALE extraction from flocculent sludge ranges normally from 90 to 190 mg/g VSS, which is only equivalent to the lowest edge of the ALE production...journal article 2022
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de Bruin, S. (author), Vasquez Cardenas, D. (author), Sarbu, S. M. (author), Meysman, F.J.R. (author), Sousa, D. Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sulfated glycosaminoglycans (sGAG) are negatively charged extracellular polymeric substances that occur in biofilms from various environments. Yet, it remains unclear whether these polymers are acquired from the external environment or produced by microbes in the biofilm. To resolve this, we analyzed the presence of sGAGs in samples of an...journal article 2022
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Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...review 2022
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Seviour, R (author), Tooker, Nick (author), Vainio, Pirjo (author), Dueholm, Morten Kam Dahl (author), Nierychlo, Marta (author), Andersen, Kasper Skytte (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author), Rossetti, S (author)Microbial communities are responsible for biological wastewater treatment, but our knowledge of their diversity and function is still poor. Here, we sequence more than 5 million high-quality, full-length 16S rRNA gene sequences from 740 wastewater treatment plants (WWTPs) across the world and use the sequences to construct the ‘MiDAS 4’...journal article 2022
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...journal article 2022
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Sharma, Keshab (author), Gutierrez, Oriol (author), Yuan, Zhiguo (author), Daelman, Matthijs R.J. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)This chapter provides a review of the models available for estimating the production and emission of methane from wastewater collection and treatment systems. The details of a number of mechanistic models as well as the simplified empirical models have been summarized. Their limitations have been identified and general methods for calibration...book chapter 2022
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Culcasi, A. (author), Ktori, R. (author), Pellegrino, A. (author), Rodriguez Pascual, M. (author), van Loosdrecht, Mark C.M. (author), Tamburini, A. (author), Cipollina, A. (author), Xevgenos, Dimitris (author), Micale, G. (author)European policy encourages the adoption of sustainable systems that promote the efficient use and recovery of minerals and chemicals. In this respect, desalination brines do contain a dramatic amount of valuable minerals and can be valorized through appropriate treatments rather than releasing them into the environment. This paper proposes an...journal article 2022
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Palmeros Parada, M.D.M. (author), van der Wielen, L.A.M. (author), Osseweijer, P. (author), van Loosdrecht, Mark C.M. (author), Pashaei Kamali, F. (author), Posada Duque, J.A. (author), van der Putten, Wim H. (author)Aviation biofuels are promising to reduce carbon emissions in the aviation sector. However, emerging concerns over biofuels indicate a need for sustainability analyses that take into consideration the context around biofuel production. Here, we present a novel ex-ante sustainability analysis of production alternatives for aviation biofuel in...book chapter 2022
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Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...journal article 2022