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Diderich, J.A. (author), Weening, S.M. (author), van den Broek, M.A. (author), Pronk, J.T. (author), Daran, J.G. (author)Saccharomyces pastorianus is an interspecies hybrid between S. cerevisiae and S. eubayanus. The identification of the parental species of S. pastorianus enabled the de novo reconstruction of hybrids that could potentially combine a wide array of phenotypic traits. Lager yeasts are characterized by their inability to decarboxylate ferulic acid...journal article 2018
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Gorter de Vries, A.R. (author), Couwenberg, Lucas G.F. (author), van den Broek, M.A. (author), De La Torre Cortés, Pilar (author), ter Horst, J. (author), Pronk, J.T. (author), Daran, J.G. (author)Targeted DNA double-strand breaks (DSBs) with CRISPR-Cas9 have revolutionized genetic modification by enabling efficient genome editing in a broad range of eukaryotic systems. Accurate gene editing is possible with near-perfect efficiency in haploid or (predominantly) homozygous genomes. However, genomes exhibiting polyploidy and/or high...journal article 2018
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Pronk, Emilie (author)Oil and gas production systems consist of flows in wells, in horizontal and inclined flowlines and in risers. Due to the reservoir composition and changes in pressure and temperature, the flow is often under multiphase conditions. With time, the reservoir matures and the reservoir pressure decreases. The gas rate drops below a critical gas rate,...master thesis 2017
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Mans, R. (author), Else-Hassing, J. (author), Wijsman, M. (author), Giezekamp, Annabel (author), Pronk, J.T. (author), Daran, J.G. (author), van Maris, A.J.A. (author)CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25...journal article 2017
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Pronk, Martijn (author)Data quality is essential in the modern world. The higher the quality of the data, the more information, knowledge and thereby wisdom can be retrieved. Besides better wisdom high quality data has another advantage: it can save costs since errors can be avoided. Low quality data is costing a lot of money to companies and will lead to information...master thesis 2017
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Pronk, Arno (author), Luo, Peng (author), Li, Q. (author), Sanders, F.C. (author), Overtoom, M.E. (author), Coar, Lancelot (author)There has been a long tradition in making ice structures, but the development of technical improvements for making ice buildings is a new field with just a handful of researchers. Most of the projects were realized by professors in cooperation with their students as part of their education in architecture and civil engineering. The following...conference paper 2017
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Wu, Yue (author), Liu, Xiuming (author), Li, Q. (author), Chen, Boxuan (author), Luo, Peng (author), Pronk, Arno (author), Mergny, Elke (author)By using inflatable moulds and then spraying cellulose-water mixture, one ice dome and two ice towers were built in Harbin in December 2016. During the whole process, form-finding of the inflatable moulds as well as the construction of these ice composite shell structures are very important for the final results.<br/>The mould for the ice dome...conference paper 2017
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Bracher, J.M. (author), de Hulster, A.F. (author), Koster, C.C. (author), van den Broek, M.A. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Biotin prototrophy is a rare, incompletely understood, and industrially relevant characteristic of Saccharomyces cerevisiae strains. The genome of the haploid laboratory strain CEN.PK113-7D contains a full complement of biotin biosynthesis genes, but its growth in biotin-free synthetic medium is extremely slow (specific growth rate [μ] ≈ 0.01...journal article 2017
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Pronk, Linard (author)At the moment, the worldwide demand for air conditioning is rapidly growing, and it is expected to exceed the demand for space heating by the 2060s. However, traditional refrigerants such as CFCs and HCFCs are regulated or phased out by the Montreal and Kyoto protocol. Secondary loop refrigerant systems use less of these harmful refrigerants...master thesis 2017
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Gorter de Vries, A.R. (author), Pronk, J.T. (author), Daran, J.G. (author)Chromosomal copy number variation (CCNV) plays a key role in evolution and health of eukaryotes. The unicellular yeast Saccharomyces cerevisiae is an important model for studying the generation, physiological impact, and evolutionary significance of CCNV. Fundamental studies of this yeast have contributed to an extensive set of methods for...journal article 2017
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Pronk, M. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid...journal article 2017
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Papapetridis, I. (author), van Dijk, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Background: Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD<sup>+</sup>-dependent acetate reduction has been shown to improve ethanol yields and...journal article 2017
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Verhoeven, M.D. (author), Lee, Misun (author), Kamoen, L. (author), van den Broek, M.A. (author), Janssen, Dick B. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)Combined overexpression of xylulokinase, pentose-phosphate-pathway enzymes and a heterologous xylose isomerase (XI) is required but insufficient for anaerobic growth of Saccharomyces cerevisiae on d-xylose. Single-step Cas9-assisted implementation of these modifications yielded a yeast strain expressing Piromyces XI that showed fast aerobic...journal article 2017
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Hakkaart, X.D.V. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)Understanding microbial growth and metabolism is a key learning objective of microbiology and biotechnology courses, essential for understanding microbial ecology, microbial biotechnology and medical microbiology. Chemostat cultivation, a key research tool in microbial physiology that enables quantitative analysis of growth and metabolism under...journal article 2017
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Brickwedde, A. (author), van den Broek, M.A. (author), Geertman, Jan Maarten A. (author), Magalhães, Frederico (author), Kuijpers, Niels G.A. (author), Gibson, Brian (author), Pronk, J.T. (author), Daran, J.G. (author)The lager brewing yeast Saccharomyces pastorianus, an interspecies hybrid of S. eubayanus and S. cerevisiae, ferments maltotriose, maltose, sucrose, glucose and fructose in wort to ethanol and carbon dioxide. Complete and timely conversion ("attenuation") of maltotriose by industrial S. pastorianus strains is a key requirement for process...journal article 2017
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Marques, W.L. (author), Mans, R. (author), Marella, Eko Roy (author), Cordeiro, Rosa Lorizolla (author), van den Broek, M.A. (author), Daran, J.G. (author), Pronk, J.T. (author), Gombert, Andreas K. (author), van Maris, A.J.A. (author)Many relevant options to improve efficacy and kinetics of sucrose metabolism in Saccharomyces cerevisiae and, thereby, the economics of sucrose-based processes remain to be investigated. An essential first step is to identify all native sucrose-hydrolysing enzymes and sucrose transporters in this yeast, including those that can be activated...journal article 2017
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Jansen, Mickel L.A. (author), Bracher, J.M. (author), Papapetridis, I. (author), Verhoeven, M.D. (author), de Bruijn, J.A. (author), de Waal, P. (author), van Maris, A.J.A. (author), Klaassen, P (author), Pronk, J.T. (author)The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. After a discussion of the challenges that these novel industrial contexts impose on yeast strains,...journal article 2017
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Gonzalez Ramos, D. (author), Gorter de Vries, A.R. (author), Grijseels, Sietske S. (author), van Berkum, M.C. (author), Swinnen, Steve (author), van den Broek, M.A. (author), Nevoigt, Elke (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)Background: Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation step that precedes ethanol production should therefore express a high and constitutive level of acetic acid tolerance before...journal article 2016
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Van Rossum, H.M. (author), Kozak, B.U. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)Saccharomyces cerevisiae is an important industrial cell factory and an attractive experimental model for evaluating novel metabolic engineering strategies. Many current and potential products of this yeast require acetyl coenzyme A (acetyl-CoA) as a precursor and pathways towards these products are generally expressed in its cytosol. The native...journal article 2016
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Pronk, T.N. (author)A recently recognized application of lattice materials is shape morphing technology, where a structure is expected to take a predefined shape in response to a change in environmental (temperature, pressure, etc.) or loading conditions. In a shape morphing structure that uses a 2D lattice material as the basis, one or more struts in the lattice...master thesis 2016