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Duque, Anouk F. (author), Bessa, Vânia S. (author), van Dongen, L.G.J.M. (author), de Kreuk, M.K. (author), Mesquita, Raquel B.R. (author), Rangel, António O.S.S. (author), van Loosdrecht, Mark C.M. (author), Castro, Paula M.L. (author)The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long-term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia-oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated...journal article 2021
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Zhang, An Ni (author), Gaston, Jeffry M. (author), Dai, Chengzhen L. (author), Zhao, Shijie (author), Poyet, Mathilde (author), Groussin, Mathieu (author), Li, Li Guan (author), van Loosdrecht, Mark C.M. (author), Zhang, Tong (author)Antibiotic resistance genes (ARGs) are widespread among bacteria. However, not all ARGs pose serious threats to public health, highlighting the importance of identifying those that are high-risk. Here, we developed an ‘omics-based’ framework to evaluate ARG risk considering human-associated-enrichment, gene mobility, and host pathogenicity....journal article 2021
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Sanawar, H. (author), Kim, L. H. (author), Farhat, N. M. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Biofouling is one of the major factors causing decline in membrane performance in reverse osmosis (RO) plants, and perhaps the biggest hurdle of membrane technology. Chemical cleaning is periodically carried out at RO membrane installations aiming to restore membrane performance. Typical cleaning agents used in the water treatment industry...journal article 2021
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van Dijk, E.J.H. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)The nitrous oxides emission was measured over 7 months in the full-scale aerobic granular sludge plant in Dinxperlo, the Netherlands. Nitrous oxide concentrations were measured in the bulk liquid and the off-gas of the Nereda® reactor. Combined with the batch wise operation of the reactor, this gave a high information density and a better...journal article 2021
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Pinel, I.S.M. (author), Hankinson, P. M. (author), Moed, D. H. (author), Wyseure, L. J. (author), Vrouwenvelder, J.S. (author), van Loosdrecht, Mark C.M. (author)Efficient control of pathogenic bacteria, specifically Legionella pneumophila, is one of the main concerns when operating industrial cooling towers. Common practices to limit proliferation involves use of disinfectants, leading to formation of disinfection by-product and increase in water corrosiveness. A disinfectant-free Legionella control...journal article 2021
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Pfaff, N.M. (author), Kleijn, J. Mieke (author), van Loosdrecht, Mark C.M. (author), Kemperman, Antoine J.B. (author)The properties of biofilm EPS are determined by the multiple interactions between its constituents and the surrounding environment. Because of the high complexity of biofilm EPS, its constituents' characterisation is still far from thorough, and identification of these interactions cannot be done yet. Therefore, we use gels of bacterial...journal article 2021
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Ochs, Pascal (author), Martin, Benjamin D. (author), Germain, Eve (author), Wu, Zhuoying (author), Lee, Po Heng (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)Suspended sludge deammonification technologies are frequently applied for sidestream ammonia removal from dewatering liquors resulting from a thermal hydrolysis anaerobic digestion (THP/AD) process. This study aimed at optimizing the operation, evaluate the performance and stability of a full-scale suspended sludge continuous stirred tank...journal article 2021
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Barrios Hernandez, M.L. (author), Bettinelli, Carolina (author), Mora-Cabrera, Karen (author), Vanegas-Camero, Maria Clara (author), Garcia, H. (author), van de Vossenberg, Jack (author), Prats, Daniel (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author)The aerobic granular sludge (AGS) process is an effective wastewater treatment technology for organic matter and nutrient removal that has been introduced in the market rapidly. Until now, limited information is available on AGS regarding the removal of bacterial and viral pathogenic organisms present in sewage. This study focussed on...journal article 2021
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Calderon Franco, D. (author), Apoorva, Seeram (author), Medema, G.J. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Wastewater treatment is challenged by the continuous emergence of chemical and biological contaminants. Disinfection, advanced oxidation, and activated carbon technologies are accessible in high-income countries to suppress them. Low-cost, easily implementable, and scalable solutions are needed for sanitation across regions. We studied the...journal article 2021
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Surkatti, Riham (author), Al Disi, Zulfa A. (author), El-Naas, Muftah H. (author), Zouari, Nabil (author), van Loosdrecht, Mark C.M. (author), Onwusogh, Udeogu (author)The gas-to-liquid (GTL) process generates considerable amounts of wastewater that are highly acidic and characterized by its high chemical oxygen demand (COD) content, due to the presence of several organic pollutants, such as alcohols, ketones, aldehydes, and fatty acids. The presence of these organics in the process water may lead to...journal article 2021
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Palmeros Parada, M.D.M. (author), van der Putten, Wim (author), van der Wielen, L.A.M. (author), Osseweijer, P. (author), van Loosdrecht, Mark C.M. (author), Pashaei Kamali, F. (author), Posada Duque, J.A. (author)Biobased production has been promoted as an alternative to fossil-based production to mitigate climate change. However, emerging concerns over the sustainability of biobased products have shown that tensions can emerge between different objectives and concerns, like emission reduction targets and food security, and that these are dependent on...journal article 2021
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Tomas Martinez, S. (author), Kleikamp, H.B.C. (author), Neu, Thomas R. (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular...journal article 2021
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Estevez Alonso, A. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Microbial community-based polyhydroxyalkanoate (PHA) production has been demonstrated repeatedly at pilot scale. Ammonium, normally present in waste streams, might be oxidized by nitrifying bacteria resulting in additional aeration energy demand. The use of low dissolved oxygen (DO) concentrations allowed to reduce nitrifying rates by up to...journal article 2021
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Kehrein, P.A. (author), van Loosdrecht, Mark C.M. (author), Osseweijer, P. (author), Posada Duque, J.A. (author)Municipal wastewater cannot any longer be perceived as a waste stream because it contains water, energy, fertilizer and other products that can be recovered with innovative technologies in so called ‘water resource factories’. Therefore a paradigm shift has been proclaimed to design water resource factories in the future that feed into a...journal article 2020
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Calderon Franco, D. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)The free-floating extracellular DNA (exDNA) fraction of microbial ecosystems harbors antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs). Natural transformation of these xenogenetic elements can generate microbial cells resistant to one or more antibiotics. Isolating and obtaining high yield exDNA is challenging due to its low...journal article 2020
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van den Berg, L. (author), Kirkland, Catherine M. (author), Seymour, Joseph D. (author), Codd, Sarah L. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Aerobic granular sludge (AGS) technology allows simultaneous nitrogen, phosphorus, and carbon removal in compact wastewater treatment processes. To operate, design, and model AGS reactors, it is essential to properly understand the diffusive transport within the granules. In this study, diffusive mass transfer within full-scale and lab-scale...journal article 2020
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Kim, Nam Kyeun (author), Mao, Ningtao (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)In this research, extracellular polymeric substances (EPS), such as EPS<sub>flocs</sub> and EPS<sub>granules</sub>, were successfully extracted from activated and aerobic granular sludge, respectively, and tested as bio-based flame retardant materials. Flax fabric was coated by the biopolymeric substances and its flammability was evaluated...journal article 2020
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Haaksman, V.A. (author), Mirghorayshi, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Operational disturbances in aerobic granular sludge (AGS) systems can result in aerobic availability of readily biodegradable COD (rbCOD). Different from activated sludge, morphological consequences on the short and long term are not well described in literature. This study investigated the effect of incomplete anaerobic uptake of acetate on...journal article 2020
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Ochs, Pascal (author), Martin, Benjamin D. (author), Germain, Eve (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)The benefits of deammonification to remove nitrogen from sidestreams, i.e., sludge dewatering liquors, in municipal wastewater treatment plants are well accepted. The ammonia removal from dewatering liquors originated from thermal hydrolysis/anaerobic digestion (THP/AD) are deemed challenging. Many different commercial technologies have been...journal article 2020
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Valk, L.C. (author), Diender, Martijn (author), Stouten, G.R. (author), Petersen, Jette F. (author), Nielsen, Per H. (author), Dueholm, Morten S. (author), Pronk, J.T. (author), van Loosdrecht, Mark C.M. (author)Acetogens have the ability to fixate carbon during fermentation by employing the Wood-Ljungdahl pathway (WLP), which is highly conserved across Bacteria and Archaea. In a previous study, product stoichometries in galacturonate-limited, anaerobic enrichment cultures of “Candidatus Galacturonibacter soehngenii,” from a novel genus within the...journal article 2020