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Vasilakou, E. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Background: Microbial metabolism is highly dependent on the environmental conditions. Especially, the substrate concentration, as well as oxygen availability, determine the metabolic rates. In large-scale bioreactors, microorganisms encounter dynamic conditions in substrate and oxygen availability (mixing limitations), which influence their...journal article 2020
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Kehrein, P.A. (author), van Loosdrecht, Mark C.M. (author), Osseweijer, P. (author), Posada Duque, J.A. (author), Dewulf, Jo (author)This paper guides decision making in more sustainable urban water management practices that feed into a circular economy by presenting a novel framework for conceptually designing and strategically planning wastewater treatment processes from a resource recovery perspective. Municipal wastewater cannot any longer be perceived as waste stream...journal article 2020
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Soler Jofra, A. (author), Laureni, M. (author), Warmerdam, Marieke (author), Pérez, Julio (author), van Loosdrecht, Mark C.M. (author)Hydroxylamine is a key intermediate in several biological reactions of the global nitrogen cycle. However, the role of hydroxylamine in anammox is still not fully understood. In this work, the impact of hydroxylamine (also in combination with other substrates) on the metabolism of a planktonic enrichment culture of the anammox species Ca....journal article 2020
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Surkatti, Riham (author), El-Naas, Muftah H. (author), van Loosdrecht, Mark C.M. (author), Benamor, Abdelbaki (author), Al-Naemi, Fatima (author), Onwusogh, Udeogu (author)Gas-to-liquid (GTL) technology involves the conversion of natural gas into several liquid hydrocarbon products. The Fischer-Tropsch (F-T) process is the most widely applied approach for GTL, and it is the main source of wastewater in the GTL process. The wastewater is generally characterized by high chemical oxygen demand (COD) and total...review 2020
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Othman, I. (author), Halim, M. H.Ab (author), Anuar, A. N. (author), Rosman, N. H. (author), Harun, H. (author), Noor, S. M. (author), Ujang, Z. (author), van Loosdrecht, Mark C.M. (author)The purpose of the microbial diversity studies conducted is to discover species composition, structure, bacterial distribution, spatial activity as well as the function and role of the microbial. A laboratory-scale sequencing batch reactor with a working volume of 2 L was used to develop aerobic granular sludge to treat livestock wastewater....journal article 2020
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Wilfert, P.K. (author), Meerdink, J. (author), Degaga, B. (author), Temmink, H. (author), Korving, L. (author), Witkamp, G.J. (author), Goubitz, K. (author), van Loosdrecht, Mark C.M. (author)Sulfide is frequently suggested as a tool to release and recover phosphate from iron phosphate rich waste streams, such as sewage sludge, although systematic studies on mechanisms and efficiencies are missing. Batch experiments were conducted with different synthetic iron phosphates (purchased Fe(III)P, Fe(III)P synthesized in the lab and...journal article 2020
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Feng, Cuijie (author), Welles, L. (author), Zhang, X. (author), Pronk, M. (author), de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author)Phosphorus has been successfully eliminated from wastewater by biological techniques of enhanced biological phosphorus removal (EBPR) process, which relies on a specific microbiota of polyphosphate accumulating organisms (PAOs) that accumulate phosphate as polyphosphates (poly-P). Most methods for quantification of poly-P pools suffer from...journal article 2020
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Barrios Hernandez, M.L. (author), Pronk, M. (author), Garcia, H. (author), Boersma, Arne (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Hooijmans, Christine M. (author)The aim of this study was to evaluate the effectiveness of the novel aerobic granular sludge (AGS) wastewater treatment technology in removing faecal indicator organisms (FIOs) compared to the conventional activated sludge (CAS) treatment system. The work was carried out at two full-scale wastewater treatment plants (WWTP) in the Netherlands,...journal article 2020
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Felz, S. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Glycosaminoglycans (GAGs) are linear heteropolysaccharides containing a derivative of an amino sugar. The possibility of the presence of GAGs in aerobic granular sludge was studied by combining SDS-PAGE with Alcian Blue staining (at pH 2.5 and 1), FTIR, mammalian Hyaluronic acid and sulfated GAG analysis kits, enzymatic digestion and specific...journal article 2020
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Pinel, I.S.M. (author), Moed, D. H. (author), Vrouwenvelder, J.S. (author), van Loosdrecht, Mark C.M. (author)Understanding the bacterial dynamics in cooling towers is imperative for the assessment of disinfection efficiency and management of microbial risks linked to aerosol formation. The objective of this study was to evaluate the impact of feed water on the cooling water bacterial microbiome and investigate the survival ability of its members...journal article 2020
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Seawater can be introduced or intrude in sewer systems and can thereby negatively influence biological wastewater treatment processes. Here we studied the impact of artificial seawater on the enhanced biological phosphate removal (EBPR) process performance by aerobic granular sludge (AGS) with synthetic wastewater. Process performance,...journal article 2020
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van Dijk, E.J.H. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author)The settling behavior of aerobic granular sludge (AGS) in full-scale reactors is different from the settling of normal activated sludge. Current activated sludge models lack the features to describe the segregation of granules based on size during the settling process. This segregation plays an important role in the granulation process and...journal article 2020
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Pinel, I.S.M. (author), Kim, Lan Hee (author), Proença Borges, Vitor R. (author), Farhat, Nadia M. (author), Witkamp, Geert Jan (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Phosphate limitation has been suggested as a preventive method against biofilms. P-limited feed water was studied as a preventive strategy against biofouling in cooling towers (CTs). Three pilot-scale open recirculating CTs were operated in parallel for five weeks. RO permeate was fed to the CTs (1) without supplementation (reference), (2)...journal article 2020
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de Jong, S.I. (author), van den Broek, M.A. (author), Merkel, Alexander Y. (author), de la Torre Cortes, Pilar (author), Kalamorz, Falk (author), Cook, Gregory M. (author), van Loosdrecht, Mark C.M. (author), McMillan, D.G.G. (author)The aerobic thermoalkaliphile Caldalkalibacillus thermarum strain TA2.A1 is a member of a separate order of alkaliphilic bacteria closely related to the Bacillales order. Efforts to relate the genomic information of this evolutionary ancient organism to environmental adaptation have been thwarted by the inability to construct a complete...journal article 2020
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Abstract: Candidatus Accumulibacter phosphatis is an important microorganism for enhanced biological phosphorus removal (EBPR). In a previous study, we found a remarkable flexibility regarding salinity, since this same microorganism could thrive in both freshwater- and seawater-based environments, but the mechanism for the tolerance to saline...journal article 2020
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Pinel, I.S.M. (author), Kleikamp, H.B.C. (author), Pabst, Martin (author), Vrouwenvelder, J.S. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids in the structural matrix of biofilms developing in engineered water systems constitute a potential target in the battle against biofouling. This report focuses specifically on the presence of sialic acids as part of the extracellular polymeric substances (EPS) of biofilms forming in cooling towers and the potential effect of...journal article 2020
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Soler Jofra, A. (author), Wang, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)A lab-scale partial nitritation granular sludge air-lift reactor was operated in continuous mode treating low strength synthetic medium (influent ca. 50 mg-N-NH<sub>4</sub> <sup>+</sup>/L). Granules were initially stratified with AOB in the external shell and NOB in the inner core at 20 °C. Once temperature was decreased progressively from 20...journal article 2019
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Conthe Calvo, M. (author), Lycus, Pawel (author), Arntzen, Magnus (author), Ramos da Silva, Aline (author), Frostegård, Åsa (author), Bakken, Lars R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The strong greenhouse gas nitrous oxide (N<sub>2</sub>O) can be emitted from wastewater treatment systems as a byproduct of ammonium oxidation and as the last intermediate in the stepwise reduction of nitrate to N<sub>2</sub> by denitrifying organisms. A potential strategy to reduce N<sub>2</sub>O emissions would be to enhance the activity of...journal article 2019
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Suresh Kumar, P. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author), Witkamp, G.J. (author)Eutrophication and the resulting formation of harmful algal blooms (HAB) causes huge economic and environmental damages. Phosphorus (P) from sewage effluent and agricultural run-off has been identified as a major cause for eutrophication. Phosphorous concentrations greater than 100 μg P/L are usually considered high enough to cause...review 2019
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Sanawar, Huma (author), Bucs, Szilárd S. (author), Pot, Martin A. (author), Zlopasa, J. (author), Farhat, Nadia M. (author), Witkamp, Geert Jan (author), Kruithof, Joop C. (author), van Loosdrecht, Mark C.M. (author), Vrouwenvelder, J.S. (author)Routine chemical cleaning with the combined use of sodium hydroxide (NaOH) and hydrochloric acid (HCl) is carried out as a means of biofouling control in reverse osmosis (RO) membranes. The novelty of the research presented herein is in the application of urea, instead of NaOH, as a chemical cleaning agent to full-scale spiral-wound RO...journal article 2019