Co-Culture of Liver-Derived Mesenchymal Stromal Cells and Intrahepatic Cholangiocyte Organoids in an Extracellular Matrix Environment: a Step Towards Complex Liver Models

To this day, liver transplantation is the only effective treatment for end-stage liver failure. Unfortunately, the scarcity of liver donors leads to waitlist mortality, pushing the need for alternative treatment options. In vitro models are essential tools in research on alternatives for transplantation. One of the promising models is the...

Repopulating a decellularized liver scaffold with liver-derived organoids in a perfusion-based bioreactor

Over the last decade new methods are explored in the field of tissue engineering to minimize the donor organ shortage. Engineering organs with a complex structure and large vascular network, such as the liver, remain a challenge. Luckily, the decellularization of an organ creates a scaffold that consists of the extracellular matrix (ECM) with...