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Abdelraheem, E.M.M. (author), Jockmann, Emely (author), Li, Jianyu (author), Günther, Stefan (author), Andexer, Jennifer N. (author), Hagedoorn, P.L. (author), Hanefeld, U. (author)S-Adenosyl-l-methionine (SAM)-dependent methyltransferases (MTs) are highly chemoselective enzymes grouped in C-, N-, O-, S- and halide MTs, depending on the (hetero) atom that acts as the methyl group acceptor. So far, OMTs present the largest group, including many well investigated candidates. The catechol OMT from mammals such as from...journal article 2023
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Enzymatic Birch Reduction via Hydrogen Atom Transfer at an Aqua-Tungsten- bis-Metallopterin CofactorSeelmann, Carola S. (author), Huwiler, Simona G. (author), Culka, Martin (author), Strampraad, M.J.F. (author), Biskup, Till (author), Weber, Stefan (author), Ullmann, G. Matthias (author), Hagedoorn, P.L. (author), Pierik, A.J. (author)The Birch reduction is a widely used synthetic tool to reduce arenes to 1,4-cyclohexadienes. Its harsh cryogenic reaction conditions and the dependence on alkali metals have motivated researchers to explore alternative approaches. In anaerobic aromatic compound degrading microbes, class II benzoyl-coenzyme A (CoA) reductases (BCRs) reduce...journal article 2023
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Coloma Hurel, J.L. (author), Hagedoorn, P.L. (author), Bento, Isabel (author), Hanefeld, U. (author)Granulicella tundricola hydroxynitrile lyase (GtHNL) is a manganese dependent cupin that catalyzes the enantioselective synthesis of cyanohydrins. The analysis of its active site shows high similarity with the active site of cupin Tm1459 from Thermotoga maritima, an enzyme that catalyzes the oxidative cleavage of styrene derivatives. GtHNL ...journal article 2023
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Hagedoorn, P.L. (author)Isothermal titration calorimetry (ITC) is a popular chemical analysis technique that can be used to measure macromolecular interactions and chemical and physical processes. ITC involves the measurement of heat flow to and from a measurement cell after each injection during a titration experiment. ITC has been useful to measure the thermodynamics...journal article 2022
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Coloma Hurel, J.L. (author), Teeuwisse, Lidwien (author), Afendi, Muhammad (author), Hagedoorn, P.L. (author), Hanefeld, U. (author)Granulicella tundricola hydroxynitrile lyase (GtHNL) catalyses the synthesis of chiral (R)‐ cyanohydrins and (R)‐β‐nitro alcohols. The triple variant GtHNL‐A40H/V42T/Q110H (GtHNL‐3V) was immobilised on Celite R‐633 and used in monophasic MTBE saturated with 100 mM KPi buffer pH 7 for the synthesis of (R)‐2‐nitro‐1‐phenylethanol (NPE) in batch...journal article 2022
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Kowalczykiewicz, Daria (author), Przypis, Marta (author), Mestrom, L. (author), Kumpf, Antje (author), Tischler, Dirk (author), Hagedoorn, P.L. (author), Hanefeld, U. (author), Jarzębski, Andrzej (author), Szymańska, Katarzyna (author)Here, we present a two-step continuous flow enzymatic synthesis process in monolithic microreactors using basic sugars as substrates. In the first step UDP-glucose pyrophosphorylase (TaGalU) catalyses the synthesis of uridine-diphosphate-glucose (UDP-Glc) using uridine triphosphate (UTP) and glucose-1-phosphate (Glc-1-P). This is followed by...journal article 2022
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Kotuniak, Radosław (author), Szczerba, Paulina (author), Sudzik, Dobromiła (author), Strampraad, M.J.F. (author), Hagedoorn, P.L. (author), Bal, Wojciech (author)We used a series of modified/substituted GGH analogues to investigate the kinetics of Cu(ii) binding to ACTUN peptides. Rules for rate modulation by 1<sup>st</sup> and 2<sup>nd</sup> sphere interactions were established, providing crucial insight into elucidation of the reaction mechanism and its contribution to biological copper transport.journal article 2022
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van Dijk, Madeleine C. (author), de Kruijff, R.M. (author), Hagedoorn, P.L. (author)Iron deficiency anemia can be treated with oral or intravenous Fe supplementation. Such supplementation has considerable effects on the human microbiome, and on opportunistic pathogenic micro-organisms. Molecular understanding of the control and regulation of Fe availability at the host-microbe interface is crucial to interpreting the side...review 2022
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Matsumura, Hirotoshi (author), Faponle, Abayomi S. (author), Hagedoorn, P.L. (author), Tosha, Takehiko (author), de Visser, Sam P. (author), Moënne-Loccoz, Pierre (author)Steady-state kinetics of cytochrome-c dependent denitrifying NO reductases (cNORs) show evidence of substrate inhibition at NO concentrations higher than 10 μM, but the mechanism of inhibition remains unclear. Here, we present low-temperature FTIR photolysis experiments carried out on the NO complex formed by addition of NO to the oxidized...journal article 2022
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Honarmand Ebrahimi, Kourosh (author), Ciofi-Baffoni, Simone (author), Hagedoorn, P.L. (author), Nicolet, Yvain (author), Le Brun, Nick E. (author), Hagen, W.R. (author), Armstrong, Fraser A. (author)A virus hijacks host cellular machineries and metabolites in order to reproduce. In response, the innate immune system activates different processes to fight back. Although many aspects of these processes have been well investigated, the key roles played by iron–sulfur [FeS] clusters, which are among the oldest classes of bio-inorganic...review 2022
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- Ji, Yuxuan (author), Wei, Li (author), Da, Anqi (author), Stark, Holger (author), Hagedoorn, P.L. (author), Ciofi-Baffoni, Simone (author), Cowley, Sally A. (author), James, William S. (author), Hagen, W.R. (author) journal article 2022
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Zhang, Jie (author), Tan, Lirong (author), Hagedoorn, P.L. (author), Wang, Ruiqi (author), Wen, Li (author), Wu, Siwei (author), Tan, Xuemei (author), Xu, Hui (author), Zhou, Xing (author)Bisphenols are important industrial materials for example for the production of plastics, but are also well known for their adverse health effects, in particular bisphenol A (BPA) is an endocrine disruptor. The widespread use of plastics has raised concerns. Therefore, the removal of bisphenols from wastewater has sparked the interest of the...journal article 2022
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Marsden, S.R. (author), Wijma, Hein J. (author), Mohr, M.K.F. (author), Justo, Inês (author), Hagedoorn, P.L. (author), Laustsen, Jesper (author), Mestrom, L. (author), McMillan, D.G.G. (author), Hanefeld, U. (author)Regulation of enzyme activity is vital for living organisms. In metalloenzymes, far-reaching rearrangements of the protein scaffold are generally required to tune the metal cofactor's properties by allosteric regulation. Here structural analysis of hydroxyketoacid aldolase from Sphingomonas wittichii RW1 (SwHKA) revealed a dynamic movement of...journal article 2022
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Varela, Romina Fernández (author), Valino, Ana Laura (author), Abdelraheem, E.M.M. (author), Medici, R. (author), Sayé, Melisa (author), Pereira, Claudio A. (author), Hagedoorn, P.L. (author), Hanefeld, U. (author), Iribarren, Adolfo (author), Lewkowicz, Elizabeth (author)In nature 2-deoxy-D-ribose-5-phosphate aldolase (DERA) catalyses the reversible formation of 2-deoxyribose 5-phosphate from D-glyceraldehyde 3-phosphate and acetaldehyde. In addition, this enzyme can use acetaldehyde as the sole substrate, resulting in a tandem aldol reaction, yielding 2,4,6-trideoxy-D-erythro-hexapyranose, which...journal article 2022
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San, H. (author), Paresoglou, M. (author), Minneboo, M.B. (author), van Hengel, I.A.J. (author), Yilmaz, A. (author), Gonzalez Garcia, Y. (author), Fluit, Ad C. (author), Hagedoorn, P.L. (author), Fratila-Apachitei, E.L. (author), Apachitei, I. (author), Zadpoor, A.A. (author)Nanoparticles (NPs) have high multifunctional potential to simultaneously enhance implant osseointegration and prevent infections caused by antibiotic-resistant bacteria. Here, we present the first report on using plasma electrolytic oxidation (PEO) to incorporate different combinations of reduced graphene oxide (rGO) and silver (Ag) NPs on...journal article 2022
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Abdelraheem, E.M.M. (author), Thair, Benjamin (author), Varela, Romina Fernández (author), Jockmann, Emely (author), Popadić, Désirée (author), Hailes, Helen C. (author), Ward, John M. (author), Hagedoorn, P.L. (author), Hanefeld, U. (author)In this review the current state-of-the-art of S-adenosylmethionine (SAM)-dependent methyltransferases and SAM are evaluated. Their structural classification and diversity is introduced and key mechanistic aspects presented which are then detailed further. Then, catalytic SAM as a target for drugs, and approaches to utilise SAM as a cofactor...review 2022
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Bouwknegt, J. (author), Koster, C.C. (author), Vos, A.M. (author), Ortiz Merino, R.A. (author), Wassink, Mats (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Hagedoorn, P.L. (author), Pronk, J.T. (author)Following publication of the original article [1], the authors reported errors in the text of the Results section and in Table 2. It refers to a mutation in a yeast gene as VPS1<sup>I410L</sup> and to the corresponding change in the Vps1 amino-acid sequence as I410L. The correct descriptions should read VPS1<sup>I401L</sup> and I401L,...journal article 2021
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Hagedoorn, P.L. (author), Hollmann, F. (author), Hanefeld, U. (author)Oleate hydratase catalyses the addition of water to the CC double bond of oleic acid to produce (R)-10-hydroxystearic acid. The enzyme requires an FAD cofactor that functions to optimise the active site structure. A wide range of unsaturated fatty acids can be hydrated at the C10 and in some cases the C13 position. The substrate scope can be...review 2021
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Estrada Bonilla, B.A. (author), Martins Costa, A.R. (author), van den Berg, D.F. (author), van Rossum, T. (author), Hagedoorn, Stefan (author), Walinga, Hielke (author), Xiao, Minfeng (author), Haas, Pieter Jan (author), Brouns, S.J.J. (author)Bacteriophages are an invaluable source of novel genetic diversity. Sequencing of phage genomes can reveal new proteins with potential uses as biotechnological and medical tools, and help unravel the diversity of biological mechanisms employed by phages to take over the host during viral infection. Aiming to expand the available collection of...journal article 2021
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Bouwknegt, J. (author), Koster, C.C. (author), Vos, A.M. (author), Ortiz Merino, R.A. (author), Wassink, Mats (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Hagedoorn, P.L. (author), Pronk, J.T. (author)Background: In most fungi, quinone-dependent Class-II dihydroorotate dehydrogenases (DHODs) are essential for pyrimidine biosynthesis. Coupling of these Class-II DHODHs to mitochondrial respiration makes their in vivo activity dependent on oxygen availability. Saccharomyces cerevisiae and closely related yeast species harbor a cytosolic Class...journal article 2021