- document
-
Bouwknegt, J. (author), Koster, C.C. (author), Vos, A.M. (author), Ortiz Merino, R.A. (author), Wassink, Mats (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Hagedoorn, P.L. (author), Pronk, J.T. (author)Following publication of the original article [1], the authors reported errors in the text of the Results section and in Table 2. It refers to a mutation in a yeast gene as VPS1<sup>I410L</sup> and to the corresponding change in the Vps1 amino-acid sequence as I410L. The correct descriptions should read VPS1<sup>I401L</sup> and I401L,...journal article 2021
- document
-
Estrada Bonilla, B.A. (author), Martins Costa, A.R. (author), van den Berg, D.F. (author), van Rossum, T. (author), Hagedoorn, Stefan (author), Walinga, Hielke (author), Xiao, Minfeng (author), Haas, Pieter Jan (author), Brouns, S.J.J. (author)Bacteriophages are an invaluable source of novel genetic diversity. Sequencing of phage genomes can reveal new proteins with potential uses as biotechnological and medical tools, and help unravel the diversity of biological mechanisms employed by phages to take over the host during viral infection. Aiming to expand the available collection of...journal article 2021
- document
-
Shams, Shams T. (author), Zhang, Jie (author), Tonin, F. (author), Hinderks, Renske (author), Deurloo, Y.N. (author), Urlacher, Vlada B. (author), Hagedoorn, P.L. (author)Lignin valorization may offer a sustainable approach to achieve a chemical industry that is not completely dependent on fossil resources for the production of aromatics. However, lignin is a recalcitrant, heterogeneous, and complex polymeric compound for which only very few catalysts can act in a predictable and reproducible manner. Laccase...journal article 2021
- document
-
Puschmann, J. (author), Mahor, D. (author), De Geus, Daniël C. (author), Strampraad, M.J.F. (author), Srour, B. (author), Hagen, W.R. (author), Todorovic, Smilja (author), Hagedoorn, P.L. (author)The heme enzyme chlorite dismutase (Cld) catalyzes O-O bond formation as part of the conversion of the toxic chlorite (ClO2-) to chloride (Cl-) and molecular oxygen (O2). Enzymatic O-O bond formation is rare in nature, and therefore, the reaction mechanism of Cld is of great interest. Microsecond timescale pre-steady-state kinetic experiments...journal article 2021
- document
-
Modaresifar, K. (author), Ganjian, M. (author), Angeloni, L. (author), Minneboo, M.B. (author), Ghatkesar, M.K. (author), Hagedoorn, P.L. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)Despite the potential of small-scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each...journal article 2021
- document
-
Hagedoorn, P.L. (author), Hollmann, F. (author), Hanefeld, U. (author)Oleate hydratase catalyses the addition of water to the CC double bond of oleic acid to produce (R)-10-hydroxystearic acid. The enzyme requires an FAD cofactor that functions to optimise the active site structure. A wide range of unsaturated fatty acids can be hydrated at the C10 and in some cases the C13 position. The substrate scope can be...review 2021
- document
-
Mahor, D. (author), Puschmann, J. (author), van den Haak, Menno (author), Kooij, Pepijn J. (author), van den Ouden, David L.J. (author), Strampraad, M.J.F. (author), Srour, B. (author), Hagedoorn, P.L. (author)In the original article published, in the g<sub>y</sub> value (column) of the H<sub>2</sub>O/OH<sup>−</sup>species (row) of Table 2 was mistakenly given as “1.18” and the correct value is “2.18”.journal article 2020
- document
-
Coloma Hurel, J.L. (author), Guiavarc'h, Y.P. (author), Hagedoorn, P.L. (author), Hanefeld, U. (author)Granulicella tundricolahydroxynitrile lyase (GtHNL) is a manganese dependent cupin which catalyses the enantioselective synthesis of (R)-cyanohydrins. TheGtHNL triple variant A40H/V42T/Q110H, previously reported to exhibit a high activity and stability, was immobilised on Celite R-633 by adsorption. The synthesis of (R)-mandelonitrile...journal article 2020
- document
-
Kotuniak, Radosław (author), Strampraad, M.J.F. (author), Bossak-Ahmad, Karolina (author), Wawrzyniak, Urszula E. (author), Ufnalska, Iwona (author), Hagedoorn, P.L. (author), Bal, Wojciech (author)The amino-terminal copper and nickel/N-terminal site (ATCUN/NTS) present in proteins and bioactive peptides exhibits high affinity towards Cu<sup>II</sup> ions and have been implicated in human copper physiology. Little is known, however, about the rate and exact mechanism of formation of such complexes. We used the stopped-flow and...journal article 2020
- document
-
Modaresifar, K. (author), Kunkels, Lorenzo (author), Ganjian, M. (author), Tümer, N. (author), Hagen, C.W. (author), Otten, L.G. (author), Hagedoorn, P.L. (author), Angeloni, L. (author), Ghatkesar, M.K. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)Recent progress in nano-/micro-fabrication techniques has paved the way for the emergence of synthetic bactericidal patterned surfaces that are capable of killing the bacteria via mechanical mechanisms. Different design parameters are known to affect the bactericidal activity of nanopatterns. Evaluating the effects of each parameter, isolated...journal article 2020
- document
-
Breve, T. (author), Filius, M. (author), Araman, Can (author), van der Helm, M. (author), Hagedoorn, P.L. (author), Joo, C. (author), van Kasteren, Sander I. (author), Eelkema, R. (author)Supramolecular encapsulation is known to alter chemical properties of guest molecules. We have applied this strategy of molecular encapsulation to temporally control the catalytic activity of a stable copper(I)–carbene catalyst. Encapsulation of the copper(I)–carbene catalyst by the supramolecular host cucurbit[7]uril (CB[7]) resulted in the...journal article 2020
- document
-
Mahor, D. (author), Puschmann, J. (author), van den Haak, Menno (author), Kooij, Pepijn J. (author), van den Ouden, David L.J. (author), Strampraad, M.J.F. (author), Srour, B. (author), Hagedoorn, P.L. (author)Abstract: Chlorite dismutase is a unique heme enzyme that catalyzes the conversion of chlorite to chloride and molecular oxygen. The enzyme is highly specific for chlorite but has been known to bind several anionic and neutral ligands to the heme iron. In a pH study, the enzyme changed color from red to green in acetate buffer pH 5.0. The...journal article 2020
- document
-
Busch, H. (author), Tonin, F. (author), Alvarenga Da Silva, N. (author), van den Broek, M.A. (author), Lu, Simona (author), Daran, J.G. (author), Hanefeld, U. (author), Hagedoorn, P.L. (author)Oleate hydratases (Ohys, EC 4.2.1.53) are a class of enzymes capable of selective water addition reactions to a broad range of unsaturated fatty acids leading to the respective chiral alcohols. Much research was dedicated to improving the applications of existing Ohys as well as to the identification of undescribed Ohys with potentially novel...journal article 2020
- document
-
Mestrom, L. (author), Marsden, S.R. (author), McMillan, D.G.G. (author), Schoevaart, Rob (author), Hagedoorn, P.L. (author), Hanefeld, U. (author)In this case study, we compare the performance of an enzyme immobilised using two different methods: i) as carrier-free catalytically active inclusion bodies or ii) as carrier-attached immobilised enzyme. To make this comparison we used a trehalose transferase from Thermoproteus uzoniensis fused to the fluorescent thermostable protein mCherry...journal article 2020
- document
-
Zhang, W. (author), Lee, Jeong Hoo (author), Younes, S.H.H. (author), Tonin, F. (author), Hagedoorn, P.L. (author), Pichler, Harald (author), Baeg, Yoonjin (author), Park, Jin-Buang (author), Hollmann, F. (author), Kourist, Robert (author)En route to a bio-based chemical industry, the conversion of fatty acids into building blocks is of particular interest. Enzymatic routes, occurring under mild conditions and excelling by intrinsic selectivity, are particularly attractive. Here we report photoenzymatic cascade reactions to transform unsaturated fatty acids into...journal article 2020
- document
-
Zhang, W. (author), Liu, H. (author), van Schie, M.M.C.H. (author), Hagedoorn, P.L. (author), Alcalde, Miguel (author), Denkova, A.G. (author), Djanashvili, K. (author), Hollmann, F. (author)It is well-known that energy-rich radiation induces water splitting, eventually yielding hydrogen peroxide. Synthetic applications, however, are scarce and to the best of our knowledge, the combination of radioactivity with enzyme-catalysis has not been considered yet. Peroxygenases utilize H2O2 as an oxidant to promote highly selective...journal article 2020
- document
-
Zhang, Jie (author), Gao, Ling (author), Hu, Jie (author), Wang, Chongjun (author), Hagedoorn, P.L. (author), Li, Ning (author), Zhou, Xing (author)Hypericin is a naturally occurring compound synthesized by certain species of the genus Hypericum, with various pharmacological effects. It is used as a natural photosensitizing agent with great potential in photodynamic therapy. This review discusses the latest results about the biosynthetic pathways and chemical synthetic routes to obtain...journal article 2020
- document
-
Zhang, Jie (author), Li, Fukun (author), Wang, Ruiqi (author), Tan, Xuemei (author), Hagedoorn, P.L. (author)Laccase is a versatile multicopper oxidase that holds great promise for many biotechnological applications. For such applications, it is essential to explore good biocatalytic systems for high activity and recyclability. The feasibility of membrane enclosed enzymatic catalysis (MEEC) for enzyme recycling with laccase was evaluated. The...journal article 2020
- document
-
La Zara, D. (author), Bailey, Maximilian R. (author), Hagedoorn, P.L. (author), Benz, D. (author), Quayle, Michael J. (author), Folestad, Staffan (author), van Ommen, J.R. (author)In this work, we report molecular layer deposition (MLD) of ultrathin poly(ethylene terephthalate) (PET) films on gram-scale batches of ultrafine particles for the first time. TiO2 P25 nanoparticles (NPs) are coated up to 50 cycles in an atmospheric-pressure fluidized-bed reactor at 150 °C using terephthaloyl chloride and ethylene glycol as...journal article 2020
- document
-
Coloma Hurel, J.L. (author), Lugtenburg, T.G. (author), Afendi, Muhammad (author), Lazzarotto, M. (author), Bracco Garcia, M.P. (author), Hagedoorn, P.L. (author), Gardossi, Lucia (author), Hanefeld, U. (author)Arabidopsis thaliana hydroxynitrile lyase (AtHNL) catalyzes the selective synthesis of (R)-cyanohydrins. This enzyme is unstable under acidic conditions, therefore its immobilization is necessary for the synthesis of enantiopure cyanohydrins. EziG Opal is a controlled porosity glass material for the immobilization of His-tagged enzymes. The...journal article 2020