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Manufacturing thin ionic polymer metal composite for sensing at the microscale

Motreuil-Ragot, P.A. (author), Hunt, A. (author), Sacco, L.N. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

Ionic polymer metal composites (IPMCs) are a class of materials with a rising appeal in biological micro-electromechanical systems (bio-MEMS) due to their unique properties (low voltage output, bio-compatibility, affinity with ionic medium). While tailoring and improving actuation capabilities of IPMCs is a key motivator in almost all IPMC...

journal article 2023

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Miniaturized engineered heart tissues from hiPSC-derived triple cell type co-cultures to study human cardiac function

Windt, L. M. (author), Wiendels, M. (author), Dostanić, M. (author), Bellin, M. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author), Mummery, C. L. (author), van Meer, B.J. (author)

Human heart tissues grown as three-dimensional spheroids and consisting of different cardiac cell types derived from pluripotent stem cells (hiPSCs) recapitulate aspects of human physiology better than standard two-dimensional models in vitro. They typically consist of less than 5000 cells and are used to measure contraction kinetics although...

journal article 2023

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An organ-on-chip device with integrated charge sensors and recording microelectrodes

Aydogmus, H. (author), Hu, M. (author), Ivancevic, Lovro (author), Frimat, Jean Philippe (author), van den Maagdenberg, Arn M.J.M. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

Continuous monitoring of tissue microphysiology is a key enabling feature of the organ-on-chip (OoC) approach for in vitro drug screening and disease modeling. Integrated sensing units are particularly convenient for microenvironmental monitoring. However, sensitive in vitro and real-time measurements are challenging due to the inherently...

journal article 2023

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Highly reproducible tissue positioning with tapered pillar design in engineered heart tissue platforms

Dostanic, M. (author), Windt, Laura (author), Wiendels, Maury (author), van Meer, Berend J. (author), Mummery, Christine L. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

We present a novel design of elastic micropillars for tissue self-assembly in engineered heart tissue (EHT) platforms. The innovative tapered profile confines reproducibly the tissue position along the main micropillar axis, increasing the accuracy of tissue contraction force measurement. Polydimethylsiloxane-based pillars were designed and...

conference paper 2023

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Rapid Prototyping of Organ-on-a-Chip Devices Using Maskless Photolithography

Kasi, D.G. (author), de Graaf, M.N.S. (author), Motreuil-Ragot, P.A. (author), Frimat, Jean-Phillipe M. S. (author), Ferrari, Michel D. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author), van den Maagdenberg, Arn M.J.M. (author), Mummery, Christine (author), Orlova, Valeria (author)

Organ-on-a-chip (OoC) and microfluidic devices are conventionally produced using microfabrication procedures that require cleanrooms, silicon wafers, and photomasks. The prototyping stage often requires multiple iterations of design steps. A simplified prototyping process could therefore offer major advantages. Here, we describe a rapid and...

journal article 2022

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Ionic polymer metal composite-based microfluidic flow sensor for bio-MEMS applications

Motreuil-Ragot, P.A. (author), Turcan, G. (author), de Wagenaar, B. (author), Hunt, A. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

Sensing flow rates in structured microenvironments like lab-on-chip (LOC) and organ-on-chip (OoC) is crucial to assess important parameters such as transport of media and molecules of interest. So far, these micro-electromechanical systems for biology (bio-MEMS) mostly rely on flow sensing systems based on thermal sensors. However, thermal...

conference paper 2022

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Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays

Revyn, N. (author), Hu, Michel H.Y. (author), Frimat, Jean-Philippe (author), de Wagenaar, B. (author), van den Maagdenberg, Arn M.J.M. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically...

conference paper 2022

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Recording 3D neuronal activity on chip with segmented 3D microelectrode arrays

Revyn, N. (author), Hu, Michel (author), Frimat, Jean-Philippe (author), van den Maagdenberg, Arn M.J.M. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

poster 2021

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FET-based integrated charge sensing in a MEM organ-on-chip platform

Aydogmus, H. (author), van Ginkel, H.J. (author), Galiti, A-D. (author), Hu, M.H.Y. (author), Frimat, J-P. (author), van den Maagdenberg, A.M.J.M. (author), Zhang, Kouchi (author), Mastrangeli, Massimo (author), Sarro, Pasqualina M (author)

poster 2021

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ForceTracker: A versatile tool for contractile force assessment in 3D organ-on-chip platforms

Rivera-Arbelaez, J.M. (author), Dostanic, M. (author), Stein, J.M. (author), van den Berg, A. (author), Segerink, L.I. (author), Mummery, C.L. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author), Ribeiro, M.C. (author), Passier, R. (author)

poster 2021

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An engineered heart tissue platform with integrated pacing microelectrodes

Dostanic, M. (author), Windt, L.M. (author), Stein, J.M. (author), van Meer, B.J. (author), Diakou, A. (author), Mummery, C.L. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

poster 2021

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Highly-conformal sputtered through-silicon vias with sharp superconducting transition

Alfaro Barrantes, J.A. (author), Mastrangeli, Massimo (author), Thoen, David (author), Visser, Sten (author), Bueno Lopez, J. (author), Baselmans, J.J.A. (author), Sarro, Pasqualina M (author)

This paper describes the microfabrication and electrical characterization of aluminum-coated superconducting through-silicon vias (TSVs) with sharp superconducting transition above 1 K. The sharp superconducting transition was achieved by means of fully conformal and void-free DC-sputtering of the TSVs with Al, and is here demonstrated in up...

journal article 2021

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Dual-Gate Fet-Based Charge Sensor Enhanced by In-Situ Electrode Decoration in a MEMS Organs-On-Chip Platform

Aydogmus, H. (author), van Ginkel, H.J. (author), Galiti, Anna-Danai (author), Hu, Michel (author), Frimat, Jean-Philippe (author), van den Maagdenberg, Arn (author), Zhang, Kouchi (author), Mastrangeli, Massimo (author), Sarro, Pasqualina M (author)

Continuous monitoring of tissue microphysiology is a key enabling feature of the organ-on-chip (OoC) approach for drug screening and disease modeling. Sensing charged species in OoC tissue microenvironments is thereby essential. However, the inherently small (i.e., cm) size of OoC devices poses the challenging requirement to integrate...

conference paper 2021

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Microelectromechanical Organs-on-Chip

Mastrangeli, Massimo (author), Aydogmus, H. (author), Dostanic, M. (author), Motreuil-Ragot, P.A. (author), Revyn, N. (author), de Wagenaar, B. (author), Dekker, R. (author), Sarro, Pasqualina M (author)

Stemming from the convergence of tissue engineering and microfluidics, organ-on-chip (OoC) technology can reproduce in vivo-like dynamic microphysiological environments for tissues in vitro. The possibility afforded by OoC devices of realistic recapitulation of tissue and organ (patho)physiology may hold the key to bridge the current...

conference paper 2021

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A Miniaturized EHT Platform for Accurate Measurements of Tissue Contractile Properties

Dostanic, M. (author), Stein, Jeroen (author), Windt, Laura (author), van Meer, Berend (author), Bellin, Milena (author), Orlova, Valeria (author), Mastrangeli, Massimo (author), Mummery, Christine (author), Sarro, Pasqualina M (author)

We present a wafer-scale fabricated, PDMS-based platform for culturing miniaturized engineered heart tissues (EHTs) which allows highly accurate measurements of the contractile properties of these tissues. The design of the platform is an anisometrically downscaled version of the Heart-Dyno system, consisting of two elastic micropillars inside...

journal article 2020

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Miniaturized engineered heart tissues from human induced pluripotent cell-derived co-culture

Windt, Laura (author), Dostanic, M. (author), Stein, Jeroen (author), Meraviglia, Viviana (author), Campostrini, Giulia (author), Bellin, Milena (author), Orlova, Valeria (author), Mastrangeli, Massimo (author), Sarro, Pasqualina M (author), van Meer, Berend (author), Mummery, Christine L. (author)

poster 2020

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Miniaturized engineered heart tissues from HiPSC-derived co-culture

Windt, Laura (author), Dostanic, M. (author), Stein, Jeroen (author), Meraviglia, Viviana (author), Campostrini, Giulia (author), Bellin, Milena (author), Orlova, Valeria (author), Mastrangeli, Massimo (author), Sarro, Pasqualina M (author), van Meer, Berend (author), Mummery, Christine (author)

poster 2020

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Superconducting High-Aspect Ratio Through-Silicon Vias with DC-Sputtered Al for Quantum 3D integration

Alfaro Barrantes, J.A. (author), Mastrangeli, Massimo (author), Thoen, David (author), Visser, Sven (author), Bueno Lopez, J. (author), Baselmans, J.J.A. (author), Sarro, Pasqualina M (author)

This paper presents the fabrication and electrical characterization of superconducting high-aspect ratio through-silicon vias DC-sputtered with aluminum. Fully conformal and void-free coating of 300 μm-deep and 50 μmwide vias with Al, a CMOS-compatible and widely available superconductor, was made possible by tailoring a funneled sidewall...

journal article 2020

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Enabling actuation and sensing in organs-on-chip using electroactive polymers

Motreuil-Ragot, P.A. (author), Hunt, A. (author), Kasi, Dhanesh (author), Brajon, B. (author), van den Maagdenberg, Arn M.J.M. (author), Orlova, Valeria (author), Mastrangeli, Massimo (author), Sarro, Pasqualina M (author)

Organ-on-chip (OoC) devices are in rising demand for high-throughput and low-cost development and toxico-logical screening of chemicals and pharmaceuticals, as they accurately mimic in vitro physiological conditions as in the human body. In particular, OoCs are urgently needed for screening cardiovascular drug toxicity. Physiological relevance...

conference paper 2020

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A 3D microelectrode array to record neural activity at different tissue depths

de Rijk, T.M. (author), Hu, Michel (author), Frimat, Jean-Philippe (author), van den Maagdenberg, Arn M.J.M. (author), Sarro, Pasqualina M (author), Mastrangeli, Massimo (author)

In vitro study of high-level neurobiological systems requires three-dimensional (3D) neuronal cultures [1]. Meas-uring responses along all three spatial dimensions is critical to record electric activity inside 3D neuronal models, such as organoids and other 3D brain tissue constructs. However, this lies beyond the capacity of 2D microelec-trode...

poster 2020