Hu, Chunyi (author), Almendros Romero, C. (author), Nam, Ki Hyun (author), Sousa Pires da Costa Basto, R.M. (author), Vink, J.N.A. (author), van Eijkeren-Haagsma, A.C. (author), Bagde, Saket R. (author), Brouns, S.J.J. (author), Ke, Ailong (author) Prokaryotes adapt to challenges from mobile genetic elements by integrating spacers derived from foreign DNA in the CRISPR array<sup>1</sup>. Spacer insertion is carried out by the Cas1–Cas2 integrase complex<sup>2–4</sup>. A substantial fraction of CRISPR–Cas systems use a Fe–S cluster containing Cas4 nuclease to ensure that spacers are...
journal article 2021
