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McKenzie, R. (author), Keizer, Emma M. (author), Vink, J.N.A. (author), van Lopik, Jasper (author), Büke, F. (author), Kalkman, Vera (author), Fleck, Christian (author), Tans, S.J. (author), Brouns, S.J.J. (author)While CRISPR-Cas defence mechanisms have been studied on a population level, their temporal dynamics and variability in individual cells have remained unknown. Using a microfluidic device, time-lapse microscopy and mathematical modelling, we studied invader clearance in Escherichia coli across multiple generations. We observed that CRISPR...journal article 2022
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Büke, F. (author), Grilli, Jacopo (author), Cosentino Lagomarsino, Marco (author), Bokinsky, G.E. (author), Tans, S.J. (author)Growth and division are central to cell size. Bacteria achieve size homeostasis by dividing when growth has added a constant size since birth, termed the adder principle, by unknown mechanisms.<sup>1</sup><sup>,</sup><sup>2</sup> Growth is well known to be regulated by guanosine tetraphosphate (ppGpp), which controls diverse processes from...journal article 2022
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D'Angelo, Francesca (author), Fernandez Fueyo, E. (author), Garcia, Pierre Simon (author), Shomar Monges, H. (author), Cebola Rebelo Manuel, R.S. (author), Büke, F. (author), van den Broek, N.J.F. (author), de Ram, C. (author), Pabst, Martin (author)Iron-sulfur (Fe-S) clusters are ancient and ubiquitous protein cofactors and play irreplaceable roles in many metabolic and regulatory processes. Fe-S clusters are built and distributed to Fe-S enzymes by dedicated protein networks. The core components of these networks are widely conserved and highly versatile. However, Fe-S proteins and...journal article 2022
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Büke, F. (author)Bacteria have been an integral part of human life since the ancient times either as cooperative tenants living in and around us or as constant threats to our health and wellbeing. Since prehistoric times we unknowingly used them as tools of fermentation and fought against them with haphazardly discovered natural remedies. Today, after more than...doctoral thesis 2020
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Noga, M.J. (author), Büke, F. (author), van den Broek, N.J.F. (author), Imholz, N.C.E. (author), Scherer, N.M.D. (author), Yang, Y.H.F. (author), Bokinsky, G.E. (author)Every cell must produce enough membrane to contain itself. However, the mechanisms by which the rate of membrane synthesis is coupled with the rate of cell growth remain unresolved. By comparing substrate and enzyme concentrations of the fatty acid and phospholipid synthesis pathways of Escherichia coli across a 3-fold range of carbon-limited...journal article 2020