Engineering ssRNA tile filaments for (dis)assembly and membrane binding

Cytoskeletal protein filaments such as actin and microtubules confer mechanical support to cells and facilitate many cellular functions such as motility and division. Recent years have witnessed the development of a variety of molecular scaffolds that mimic such filaments. Indeed, filaments that are programmable and compatible with biological...

Single-Molecule Structure and Topology of Kinetoplast DNA Networks

Kinetoplast DNA (kDNA) is a two-dimensional Olympic-ring-like network of mutually linked DNA minicircles found in certain parasites called trypanosomes. Understanding the self-assembly and replication of this structure are not only major open questions in biology but can also inform the design of synthetic topological materials. Here, we...

High-Speed Tapping Mode AFM Utilizing Recovery of Tip-Sample Interaction

We propose to use the State Estimation by Sum-of-Norms Regularisation (STATESON-)algorithm for recovering the tip-sample interaction in high-speed tapping mode atomic force microscopy (AFM). This approach enables accurate sample height estimation for each independent cantilever oscillation period, provided that the tip-sample interaction...

Filtering and Imaging of Frequency-Degenerate Spin Waves Using Nanopositioning of a Single-Spin Sensor

Nitrogen-vacancy (NV) magnetometry is a new technique for imaging spin waves in magnetic materials. It detects spin waves by their microwave magnetic stray fields, which decay evanescently on the scale of the spin-wavelength. Here, we use nanoscale control of a single-NV sensor as a wavelength filter to characterize frequency-degenerate spin...

Bridging-induced phase separation induced by cohesin SMC protein complexes

Structural maintenance of chromosome (SMC) protein complexes are able to extrude DNA loops. While loop extrusion constitutes a fundamental building block of chromosomes, other factors may be equally important. Here, we show that yeast cohesin exhibits pronounced clustering on DNA, with all the hallmarks of biomolecular condensation. DNA...

Directional Excitation of a High-Density Magnon Gas Using Coherently Driven Spin Waves

Controlling magnon densities in magnetic materials enables driving spin transport in magnonic devices. We demonstrate the creation of large, out-of-equilibrium magnon densities in a thin-film magnetic insulator via microwave excitation of coherent spin waves and subsequent multimagnon scattering. We image both the coherent spin waves and the...

Free-Space Subterahertz-Field Polarization Controlled by Selection of Waveguide Modes

We experimentally study the free-space electromagnetic field emitted from a multimode rectangular waveguide equipped with a diagonal-horn antenna. Using the frequency range of 215-580 GHz, a photomixer is used to launch a free-space circularly polarized electromagnetic field, exciting multiple modes at the input of the rectangular waveguide...

Semi-permeability of graphene nanodrums in sucrose solution

Semi-permeable membranes are important elements in water purification and energy generation applications, for which the atomic thickness and strength of graphene can enhance efficiency and permeation rate while maintaining good selectivity. Here, we show that an osmotic pressure difference forms across a suspended graphene membrane as a...

Publisher Correction: The condensin holocomplex cycles dynamically between open and collapsed states (Nature Structural & Molecular Biology, (2020), 27, 12, (1134-1141), 10.1038/s41594-020-0508-3)

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Analysis of a single-mode waveguide at sub-terahertz frequencies as a communication channel

We study experimentally the transmission of an electromagnetic waveguide in the frequency range from 160 to 300 GHz. Photo-mixing is used to excite and detect the fundamental TE₁₀ mode in a rectangular waveguide with two orders-of-magnitude lower impedance. The large impedance mismatch leads to a strong frequency dependence of the...

Local Electrodynamics of a Disordered Conductor Model System Measured with a Microwave Impedance Microscope

We study the electrodynamic impedance of percolating conductors with a predefined network topology using a scanning microwave impedance microscope at gigahertz frequencies. For a given percolation number we observe strong spatial variations across a sample that correlate with the connected regions (clusters) in the network when the...

Shielded cantilever with on-chip interferometer circuit for THz scanning probe impedance microscopy

We have realized a microstrip based terahertz (THz) near field cantilever that enables quantitative measurements of the impedance of the probe tip at THz frequencies (0.3 THz). A key feature is the on-chip balanced hybrid coupler that serves as an interferometer for passive signal cancellation to increase the readout circuit sensitivity...

Visualization of unstained DNA nanostructures with advanced in-focus phase contrast TEM techniques

Over the last few years, tremendous progress has been made in visualizing biologically important macromolecules using transmission electron microscopy (TEM) and understanding their structure-function relation. Yet, despite the importance of DNA in all forms of life, TEM visualization of individual DNA molecules in its native unlabeled form...

Distortion of DNA Origami on Graphene Imaged with Advanced TEM Techniques

While graphene may appear to be the ultimate support membrane for transmission electron microscopy (TEM) imaging of DNA nanostructures, very little is known if it poses an advantage over conventional carbon supports in terms of resolution and contrast. Microscopic investigations are carried out on DNA origami nanoplates that are supported...

Spatial conductivity mapping of unprotected and capped black phosphorus using microwave microscopy

Thin layers of black phosphorus present an ideal combination of a 2Dmaterial with a tunable direct bandgap and high carrier mobility. However the material suffers from degradation in ambient conditions due to an oxidation reaction which involves water, oxygen and light. Wehave measured the spatial profile of the conductivity on flakes of...

Condensin Smc2-Smc4 Dimers Are Flexible and Dynamic

Structural maintenance of chromosomes (SMC) protein complexes, including cohesin and condensin, play key roles in the regulation of higher-order chromosome organization. Even though SMC proteins are thought to mechanistically determine the function of the complexes, their native conformations and dynamics have remained unclear. Here, we probe...

High-Speed AFM Reveals the Dynamics of Single Biomolecules at the Nanometer Scale

Atomic force microscopy allows visualization of biomolecules with nanometer resolution under physiological conditions. Recent advances have improved the time resolution of the technique from minutes to tens of milliseconds, meaning that it is now possible to watch single biomolecules in action in real time. Here, we review this development.