Searched for: author:"Kim, S.H."
(1 - 10 of 10)
document
Kim, S.H. (author), Ganji, M. (author), Kim, E. (author), van der Torre, J. (author), Abbondanzieri, E. (author), Dekker, C. (author)
The three-dimensional organization of DNA is increasingly understood to play a decisive role in vital cellular processes. Many studies focus on the role of DNA-packaging proteins, crowding, and confinement in arranging chromatin, but structural information might also be directly encoded in bare DNA itself. Here, we visualize plectonemes ...
journal article 2018
document
Kim, S.H. (author), Ahn, Tak Kyoon (author), Cui, T.J. (author), Chauhan, S. (author), Sung, Jaeyoung (author), Joo, C. (author), Kim, Doseok (author)
At the core of homologous DNA repair, RecA catalyzes the strand exchange reaction. This process is initiated by a RecA loading protein, which nucleates clusters of RecA proteins on single-stranded DNA. Each cluster grows to cover the single-stranded DNA but may leave 1- to 2-nucleotide (nt) gaps between the clusters due to three different...
journal article 2017
document
Vlijm, R. (author), Kim, S.H. (author), De Zwart, P. L. (author), Dalal, Y. (author), Dekker, C. (author)
Nucleosomes form the unit structure of the genome in eukaryotes, thereby constituting a fundamental tenet of chromatin biology. In canonical nucleosomes, DNA wraps around the histone octamer in a left-handed toroidal ramp. Here, in single-molecule magnetic tweezers studies of chaperone-assisted nucleosome assembly, we show that the handedness...
journal article 2017
document
Ganji, M. (author), Kim, S.H. (author), van der Torre, J. (author), Abbondanzieri, E. (author), Dekker, C. (author)
DNA supercoiling crucially affects cellular processes such as DNA replication, gene expression, and chromatin organization. However, mechanistic understanding of DNA supercoiling and the related DNA-processing enzymes has remained limited, mainly due to the lack of convenient experimental tools to probe these phenomena. Here, we report a...
journal article 2016
document
Kim, S.H. (author), Vlijm, R. (author), van der Torre, J. (author), Dalal, Y. (author), Dekker, C. (author)
Centromere-specific nucleosomes are a central feature of the kinetochore complex during mitosis, in which microtubules exert pulling and pushing forces upon the centromere. CENP-A nucleosomes have been assumed to be structurally unique, thereby providing resilience under tension relative to their H3 canonical counterparts. Here, we directly test...
journal article 2016
document
Kim, S.H. (author), Park, J. (author), Joo, C. (author), Kim, D. (author), Ha, T. (author)
RecA proteins form a long stable filament on a single-stranded DNA and catalyze strand exchange reaction. The stability of RecA filament changes dramatically with pH, yet its detailed mechanism is not known. Here, using a single molecule assay, we determined the binding and dissociation rates of RecA monomers at the filament ends at various pH....
journal article 2015
document
Choi, Y.S. (author), Seo, K.W. (author), Lee, S.J. (author), Kim, S.H. (author), Yun, J.H. (author)
The flows in a naturally evaporating liquid droplet confined by two flat plates were investigated experimentally. A diluted sodium-chloride (NaCl) aqueous solution was used to form the confined droplets. The evaporation of water at the free surface of the droplet built up a concentration gradient inside the solution, which induced a natural...
conference paper 2013
document
Kim, S.H. (author), Joo, C. (author), Ha, T. (author), Kim, D. (author)
A is a DNA-dependent ATPase and mediates homologous recombination by first forming a filament on a single-stranded (ss) DNA. RecA binds preferentially to TGG repeat sequence, which resembles the recombination hot spot Chi (5'-GCTGGTGG-3') and is the most frequent pattern (GTG) of the codon usage in Escherichia coli. Because of the highly dynamic...
journal article 2013
document
Kim, Dong Jin (author), Kim, S.Y. (author), You, Y.J. (author), Rhee, K.P. (author), Kim, S.H. (author), Kim, Y.G. (author)
journal article 2013
document
Kim, D.S. (author), Kim, M.K. (author), Kim, S.H. (author), Choo, Y.W. (author)
The investigation on the seismic behavior of dams becomes crucial but is limited due to lack of experimental or field data. This paper aims to experimentally simulate the seismic behavior of three types of dams; earth-core rock-fill dam (ECRD), concrete-faced rock-fill dam (CFRD), and composite dam which combines concrete dam and CFRD. A series...
conference paper 2012
Searched for: author:"Kim, S.H."
(1 - 10 of 10)