Searched for: author:"Soloviev, O.A."
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Pozzi, Paolo (author), Smith, C.S. (author), Carroll, E.C.M. (author), Wilding, Dean (author), Soloviev, O.A. (author), Booth, Martin (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
Inhomogeneities in the refractive index of a biological microscopy sample can introduce phase aberrations, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve image quality. However, conventional adaptive optics can only correct a single phase aberration for the whole field of...
journal article 2020
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Nguyen, Hieu Thao (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author)
We present an efficient phase retrieval approach for imaging systems with high numerical aperture based on the vectorial model of the point spread function. The algorithm is in the class of alternating minimization methods and can be adjusted for applications with either known or unknown amplitude of the field in the pupil. The algorithm...
journal article 2020
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Pozzi, P. (author), Maddalena, L. (author), Ceffa, N.G. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Carroll, E.C.M. (author), Verhaegen, M.H.G. (author)
The use of spatial light modulators to project computer generated holograms is a common strategy for optogenetic stimulation of multiple structures of interest within a three-dimensional volume. A common requirement when addressing multiple targets sparsely distributed in three dimensions is the generation of a points cloud, focusing excitation...
journal article 2019
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Piscaer, P.J. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author)
A new wavefront sensorless adaptive optics method is presented that can accurately correct for time-varying aberrations using a single focal plane image at each sample instance. The linear relation between the mean square of the aberration gradient and the change in second moment of the image forms the basis of the presented method. The new...
journal article 2019
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Pozzi, Paolo (author), Soloviev, O.A. (author), Wilding, Dean (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image...
journal article 2018
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Piscaer, P.J. (author), Gupta, A. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author)
In this paper, we propose the use of Gaussian radial basis functions (GRBFs) to model the generalized pupil function for phase retrieval. The selection of the GRBF hyper-parameters is analyzed to achieve an increased accuracy of approximation. The performance of the GRBF-based method is compared in a simulation study with another modal-based...
journal article 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned...
journal article 2018
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and...
conference paper 2018
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Kazasidis, Orestis (author), Verpoort, Sven (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author), Wittrock, Ulrich (author)
With a view to the next generation of large space telescopes, we investigate guide-starfree, image-based aberration correction using a unimorph deformable mirror in a plane conjugate to the primary mirror.We designed and built a high-resolution imaging testbed to evaluate control algorithms. In this paper we use an algorithm based on the...
journal article 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...
conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)
The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...
conference paper 2018
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Gong, H. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
We present a quantitative phase imaging microscope based on a Shack-Hartmann sensor, that directly reconstructs the optical path difference (OPD) in reflective mode. Comparing with the holographic or interferometric methods, the SH technique needs no reference beam in the setup, which simplifies the system. With a preregistered reference, the...
journal article 2017
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Wilding, D. (author), Soloviev, O.A. (author), Pozzi, P. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
A methodology for retrieving the unknown object distribution and point-spread functions (PSFs) from a set of images acquired in the presence of temporal phase aberrations is presented in this paper. The method works by finding optimal complimentary linear filters for multi-frame deconvolution. The algorithm uses undemanding computational...
journal article 2017
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Wilding, D. (author), de Iongh, Gijs (author), Soloviev, O.A. (author), Pozzi, P. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
By taking multiple input-output measurements, it is shown how to determine the input to an optical system that corrects unknown phase aberrations without interferometric measurements or online iterative optimization within a couple of seconds. It is shown to work in simulations and experiment. This technique may also be used to acquire the...
conference paper 2017
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Gong, H. (author), Agbana, T.E. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
In this Letter, we show that a Shack-Hartmann wavefront sensor can be used for the quantitative measurement of the specimen optical path difference (OPD) in an ordinary incoherent optical microscope, if the spatial coherence of the illumination light in the plane of the specimen is larger than the microscope resolution. To satisfy this...
journal article 2017
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in...
conference paper 2016
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is...
conference paper 2016
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Gong, H. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
We present a reference-less and time-multiplexing phase retrieval method by making use of the digital micromirror device (DMD). In this method, the DMD functions not only as a flexible binary mask which modulates the optical field, but also as a sampling mask for measuring corresponding phases, which makes the whole setup simple and robust. The...
conference paper 2016
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Agbana, T.E. (author), Yang, H. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
This paper presents experimental results of a static aberration control algorithm based on the linear relation be- tween mean square of the aberration gradient and the second moment of point spread function for the generation of control signal input for a deformable mirror (DM). Results presented in the work of Yang et al.1 suggested a good...
conference paper 2016
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Gong, H. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovine, G.V. (author)
We investigate the possibility of interferometric optical field sensor with DMD forming a series of sampling interferometers in the pupil of an optical system. Our preliminary experiment validates the feasibility of our approach for the field reconstruction in the case of coherent field. We believe that this approach can be extended to sensing...
conference paper 2015
Searched for: author:"Soloviev, O.A."
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