The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading

Background Clostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ?10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which...