Searched for: author%3A%22Vdovin%2C+Gleb%22
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Soloviev, O.A. (author), Noom, J. (author), Nguyen, Hieu Thao (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
We investigate the general adjustment of projection-based phase retrieval algorithms for use with saturated data. In the phase retrieval problem, model fidelity of experimental data containing a non-zero background level, fixed pattern noise, or overexposure, often presents a serious obstacle for standard algorithms. Recently, it was shown...
conference paper 2022
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Agbana, T.E. (author), Nijman, Patrick (author), Hoeboer, M.D.B. (author), van Grootheest, D.N. (author), van Diepen, Angela (author), van Lieshout, Lisette (author), Diehl, J.C. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
We present a simple method for the diagnosis of urinary schistosomiasis using an in-line lensless holographic microscope combined with flow cytometry technique. Using simple image processing algorithms and binary image classifier, our system provides automated detection of Schistosoma haematobium eggs in infected urine samples. Registered...
conference paper 2020
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Heemels, Alex (author), Agbana, T.E. (author), Pereira, S.F. (author), Diehl, J.C. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
Fourier Ptychography is a computational imaging technique able to decouple high resolution from wide field of view, bypassing the diffraction limit of the microscope. Since it does not rely on high precision mechanics or fluorescent imaging, it is of practical interest for implementation in low scale devices. Despite its gains, realizing a...
conference paper 2020
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Pozzi, P. (author), Smith, C.S. (author), Carroll, E.C.M. (author), Wilding, D. (author), Soloviev, O.A. (author), Booth, Martin (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
Inhomogeneities in the refractive index of a biological microscopy sample can introduce phase aberrations, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve image quality. However, conventional adaptive optics can only correct a single phase aberration for the whole field of...
journal article 2020
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Pozzi, P. (author), Maddalena, L. (author), Ceffa, N.G. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Carroll, E.C.M. (author), Verhaegen, M.H.G. (author)
The use of spatial light modulators to project computer generated holograms is a common strategy for optogenetic stimulation of multiple structures of interest within a three-dimensional volume. A common requirement when addressing multiple targets sparsely distributed in three dimensions is the generation of a points cloud, focusing excitation...
journal article 2019
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...
conference paper 2018
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and...
conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned...
journal article 2018
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Kazasidis, Orestis (author), Verpoort, Sven (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author), Wittrock, Ulrich (author)
With a view to the next generation of large space telescopes, we investigate guide-starfree, image-based aberration correction using a unimorph deformable mirror in a plane conjugate to the primary mirror.We designed and built a high-resolution imaging testbed to evaluate control algorithms. In this paper we use an algorithm based on the...
journal article 2018
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Agbana, T.E. (author), Diehl, J.C. (author), van Pul, Fiona (author), Khan, Shahid M. (author), Patlan, Vsevolod (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
We have optimized the design and imaging procedures, to clearly resolve the malaria parasite in Giemsa-stained thin blood smears, using simple low-cost cellphone-based microscopy with oil immersion. The microscope uses a glass ball as the objective and the phone camera as the tube lens. Our optimization includes the optimal choice of the ball...
journal article 2018
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Pozzi, P. (author), Soloviev, O.A. (author), Wilding, D. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image...
journal article 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)
The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...
conference paper 2018
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Taballione, Caterina (author), Agbana, T.E. (author), Vdovin, Gleb (author), Hoekman, Marcel (author), Wevers, Lennart (author), Kalkman, J. (author), Verhaegen, M.H.G. (author), van der Slot, Peter J.M. (author), Boller, Klaus-Jochen (author)
Wavelength meters are central for many applications such as in telecommunication systems or laser monitoring. The primary function of a wavelength meter is to provide an output signal that changes sensitively with the wavelength of the input light. Of central importance is the reproducibility of the output signal even in the presence of external...
poster 2017
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Wilding, D. (author), de Iongh, Gijs (author), Soloviev, O.A. (author), Pozzi, P. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
By taking multiple input-output measurements, it is shown how to determine the input to an optical system that corrects unknown phase aberrations without interferometric measurements or online iterative optimization within a couple of seconds. It is shown to work in simulations and experiment. This technique may also be used to acquire the...
conference paper 2017
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Agbana, T.E. (author), Gong, H. (author), Amoah, A.S. (author), Bezzubik, V (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
journal article 2017
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Taballione, C. (author), Agbana, T.E. (author), Vdovin, Gleb (author), Hoekman, M (author), Wevers, L. (author), Kalkman, J. (author), Verhaegen, M.H.G. (author), van der Slot, P.J.M. (author), Boller, KJ (author)
conference paper 2017
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Wilding, D. (author), Soloviev, O.A. (author), Pozzi, P. (author), Vdovin, Gleb (author), Verhaegen, M.H.G. (author)
A methodology for retrieving the unknown object distribution and point-spread functions (PSFs) from a set of images acquired in the presence of temporal phase aberrations is presented in this paper. The method works by finding optimal complimentary linear filters for multi-frame deconvolution. The algorithm uses undemanding computational...
journal article 2017
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Gong, H. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
We present a quantitative phase imaging microscope based on a Shack-Hartmann sensor, that directly reconstructs the optical path difference (OPD) in reflective mode. Comparing with the holographic or interferometric methods, the SH technique needs no reference beam in the setup, which simplifies the system. With a preregistered reference, the...
journal article 2017
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Gong, H. (author), Agbana, T.E. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
In this Letter, we show that a Shack-Hartmann wavefront sensor can be used for the quantitative measurement of the specimen optical path difference (OPD) in an ordinary incoherent optical microscope, if the spatial coherence of the illumination light in the plane of the specimen is larger than the microscope resolution. To satisfy this...
journal article 2017
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Gong, H. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovin, Gleb (author)
We present a reference-less and time-multiplexing phase retrieval method by making use of the digital micromirror device (DMD). In this method, the DMD functions not only as a flexible binary mask which modulates the optical field, but also as a sampling mask for measuring corresponding phases, which makes the whole setup simple and robust. The...
conference paper 2016
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