Deep-tissue label-free quantitative optical tomography

In vivo imaging of small animals is of wide interest to the biomedical community studying biological disease and developmental processes. However, optical imaging deep in tissue is severely limited by light scattering, posing restrictions on the imaging depth, image contrast, and spatial resolution. We demonstrate optical coherence projection...

High-resolution deep-tissue quantitative optical tomography

Optical imaging is one of the primary tools in biological and medical research. Over the years many different optical imaging modalities have been developed that have driven the imaging performance in terms of image resolution, contrast, imaging time, and maximum allowed sample size. The goal of this work is to develop techniques for 3D optical...

Comparison of image reconstruction techniques for optical projection tomography

We present a comparison of image reconstruction techniques for optical projection tomography. We compare conventional filtered back projection, sinogram filtering using the frequency–distance relationship (FDR), image deconvolution, and 2D point-spread-function-based iterative reconstruction. The latter three methods aim to remove the spatial...

Optical coherence computed tomography for quantitative 3D imaging of adult zebrafish (Withdrawal Notice)

Point spread function based image reconstruction in optical projection tomography

As a result of the shallow depth of focus of the optical imaging system, the use of standard filtered back projection in optical projection tomography causes space-variant tangential blurring that increases with the distance to the rotation axis. We present a novel optical tomographic image reconstruction technique that incorporates the point...

Image resolution and deconvolution in optical tomography

We present a frequency domain analysis of the image resolution of optical tomography systems. The result of our analysis is a description of the spatially-variant resolution in optical tomographic image after reconstruction as a function of the properties of the imaging system geometry. We validate our model using optical projection...

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