Immobilization protects enzymes from plasma-mediated inactivation

Non-thermal plasmas are used in various applications to inactivate biological agents or biomolecules. A complex cocktail of reactive species, (vacuum) UV radiation and in some cases exposure to an electric field together cause the detrimental effects. In contrast to this disruptive property of technical plasmas, we have shown previously that...

Creating Conjugated C−C Bonds between Commercial Carbon Electrode and Molecular Catalyst for Oxygen Reduction to Hydrogen Peroxide

Immobilizing molecular catalysts on electrodes is vital for electrochemical applications. However, creating robust electrode-catalyst interactions while maintaining good catalytic performance and rapid electron transfer is challenging. Here, without introducing any foreign elements, we show a bottom-up synthetic approach of constructing the...

Immobilization and Application of Fatty Acid Photodecarboxylase in Deep Eutectic Solvents

Since its discovery in 2017, the fatty acid decarboxylase (FAP) photoenzyme has been the focus of extensive research, given its ability to convert fatty acids into alka(e)nes using merely visible blue light. Unfortunately, there are still some drawbacks that limit the applicability of this biocatalyst, such as poor solubility of the...

Immobilization of Prunus amygdalus Hydroxynitrile Lyase on Celite

The hydroxynitrile lyase from Prunus amygdalus was immobilized on Celite R-633. The immobilized enzyme could successfully be utilized in buffer saturated MTBE and excellent conversions of benzaldehyde to R-mandelonitrile were observed. No leaching occurred. To achieve high enantioselectivities, the suppression of the undesired background...

Effects of Substrate and Polymer Encapsulation on CO₂ Electroreduction by Immobilized Indium(III) Protoporphyrin

Heterogenization of molecular catalysts for CO₂ electroreduction has attracted significant research activity, due to the combined advantages of homogeneous and heterogeneous catalysts. In this work, we demonstrate the strong influence of the nature of the substrate on the selectivity and reactivity of electrocatalytic CO<sub>2</sub...

Production and immobilization of lipase PCL and its application in synthesis of α‐linolenic acid‐rich diacylglycerol

α‐Linolenic acid‐rich diacylglycerol has been demonstrated with promising health promotion functions. This study examined the production, immobilization of lipase PCL, and its application in the synthesis of diacylglycerol by esterification of α‐linolenic acid with glycerol. The resin ECR8806 was selected as an effective support for the...

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)

Cross-linked enzyme aggregates (CLEAs) have many economic and environmental benefits in the context of industrial biocatalysis. They are easily prepared from crude enzyme extracts, and the costs of (often expensive) carriers are circumvented. They generally exhibit improved storage and operational stability towards denaturation by heat, organic...

Deposition and atomic force microscopy of individual phthalocyanine polymers between nanofabricated electrodes

Preparation of D-xylulose from D-xylose: Enzyme and Microbial Technology