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Localization and kinetics of pyruvate-metabolizing enzymes in relation to aerobic alcoholic fermentation in Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621

Van Urk, H. (author), Schipper, D. (author), Breddveld, G.J. (author), Mak, P.R. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1989

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Enzymic analysis of the crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae

Postma, E. (author), Verduyn, C. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1989

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Preparation of D-xylulose from D-xylose: Enzyme and Microbial Technology

Pronk, J.T. (author), Bakker, A.W. (author), Van Dam, H.E. (author), Straathof, A.J.J. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1988

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Hydrogen Peroxide Metabolism in Yeasts

Verduyn, C. (author), Giuseppin, M.L. (author), Scheffers, W.A. (author), van Dijken, J.P. (author)

journal article 1988

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Production, Distribution, and Kinetic Properties of Inulinase in Continuous Cultures of Kluyveromyces marxianus CBS 6556

Rouwenhorst, R.J. (author), Visser, L.E. (author), Van Der Baan, A.A. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author)

journal article 1988

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Method for producing ethanol from xylose-containing substance

Van Dijken, J. (author), Scheffers, W.A. (author)

A method for producing ethanol from a D-xylose containing substance, comprising fermenting said substance with a yeast of the genus Pichia or its imperfect forms belonging to the genus Candida, selected from the species consisting of Pichia stipitis, Pichia segobiensis and Candida shehatae, under aerobic or anaerobic conditions to produce...

patent 1987

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Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts

De Jonge, P. (author), De Jongh, F.C.M. (author), Meijers, R. (author), Steensma, H.Y. (author), Scheffers, W.A. (author)

Chromosomal DNAs from various yeast species were separated by orthogonal-field-alternation gel electrophoresis (OFAGE). To this end we developed a spheroplasting and lysis method to obtain intact DNA from both ascomycetous and basidiomycetous yeasts. The OFAGE banding patterns of22 ascomycetous and four basidiomycetous yeast strains were...

journal article 1986

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Mechanism of control of adenylate cyclase activity in yeast by fermentable sugars and carbonyl cyanide m-chlorophenylhydrazon

Purwin, C. (author), Nicolay, K. (author), Scheffers, W.A. (author), Holzer, H. (author)

journal article 1986

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Alcoholic fermentation by 'non-fermentative' yeasts

Van Dijken, J.P. (author), Van den Bosch, E. (author), Hermans, J.J. (author), Rodrigues de Miranda, L. (author), Scheffers, W.A. (author)

journal article 1986

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Redox balances in the metabolism of sugars by yeasts. (NAD(H); NADP(H); glucose metabolism; xylose fermentation; ethanol; Crabtree effect; Custers effect)

Van Dijken, J.P. (author), Scheffers, W.A. (author)

journal article 1986

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Multiple forms of xylose reductase in Pachysolen tannophilus CBS4044 (Xylose reductase; xylose fermentation; yeast; wood sugar; ethanol)

Verduyn, C. (author), Frank Jzn, J. (author), Van Dijken, J.P. (author), Scheffers, W.A. (author)

journal article 1985

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Critical Parameters in the Isolation of Mitochondria from Candida-Utilis Grown in Continuous Culture

Bruinenberg, P.M. (author), Van Dijken, J.P. (author), Kuenen, J.G. (author), Scheffers, W.A. (author)

journal article 1985

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Oxidation of NADH and NADPH by Mitochondria from the Yeast Candida utilis

Bruinenberg, P.M. (author), Van Dijken, J.P. (author), Kuenen, J.G. (author), Scheffers, W.A. (author)

journal article 1985

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Properties of the NAD(P)H-dependent xylose reductase from the xylosefermenting yeast Pichia stipitis

Verduyn, C. (author), Van Kleef, R. (author), Frank Jzn, J. (author), Schreuder, H. (author), Van Dijken, J.P. (author), Scheffers, W.A. (author)

Xylose reductase from the xylose-fermenting yeast Pichia stipitis was purified to electrophoretic and spectral homogeneity via ion-exchange, affinity and highperformance gel chromatography. The enzyme was active with various aldose substrates, such as DL-glyceraldehyde, L-arabinose, D-xylose, D-ribose, D-galactose and D-glucose. Hence the xylose...

journal article 1985

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Alcoholic Fermentation of d-Xylose by Yeasts

Toivola, A. (author), Yarrow, D. (author), van den Bosch, E. (author), van Dijken, J.P. (author), Scheffers, W.A. (author)

journal article 1984

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Inhibition of fermentation and growth in batch cultures of the yeast Brettanomyces intermedius upon a shift from aerobic to anaerobic conditions (Custers effect)

Wijsman, M.R. (author), Van Dijken, J.P. (author), Van Kleeff, B.H.A. (author), Scheffers, W.A. (author)

journal article 1984

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NADH-linked aldose reductase: The key to anaerobic alcoholic fermentation of xylose by yeasts

Bruinenberg, P.M. (author), De Bot, P.H.M. (author), Van Dijken, J.P. (author), Scheffers, W.A. (author)

The kinetics and enzymology of o-xylose utilization were studied in aerobic and anaerobic batch cultures of the facultatively fermentative yeasts Candida utilis, Pachysolen tannophilus, and Pichia stipitis. These yeasts did not produce ethanol under aerobic conditions. When shifted to anaerobiosis cultures of C. utilis did not show fermentation...

journal article 1984

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A theoretical analysis of NADPH production and consumption in yeasts

Bruinenberg, P.M. (author), Van Dijken, J.P. (author), Scheffers, W.A. (author)

Theoretical calculations of the NADPH requirement for yeast biomass formation reveal that this parameter is strongly dependent on the carbon and nitrogen source. The data obtained have been used to estimate the carbon flow over the NADPH-producing pathways in these organisms, namely the hexose monophosphate pathway and the NADP+-linked...

journal article 1983

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An enzymic analysis of NADPH production and consumption in Candida utilis

Bruinenberg, P.M. (author), Van Dijken, J. (author), Scheffers, W.A. (author)

journal article 1983

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Phosphorus-31 nuclear magnetic resonance studies of intracellular pH, phosphate compartmentation and phosphate transport in yeasts

Nicolay, K. (author), Scheffers, W.A. (author), Bruinenberg, P.M. (author), Kaptein, R. (author)

31p NMR spectra were obtained from suspensions of Candida utilis, Saccharomyces cerevisiae and Zygosaccharomyces bailii grown aerobically on glucose. Direct introduction of substrate into the cell suspension, without interruption of the measurements, revealed rapid changes in pH upon addition of the energy source. All 3 ~p NMR spectra of the...

journal article 1982