Computational modeling and optimization of biopharmaceutical downstream processes

Vaccination is a strong and effective way to prevent spreading of infectious diseases and promotes global health. In the future, the importance of vaccines is expected only to increase, driven by factors such as increased international traveling, higher healthcare expenditures, and a growing population. To meet the growing demands, it is...

Electrochemical Ammonia Synthesis: Hydrogen Permeable Electrodes as Alternative Pathway for Nitrogen Reduction

In the last century, the indiscriminate use of fossil energy to power the industrial revolution and technological progress of humankind, has led to the depletion of limited natural resources and, most importantly, the emission and accumulation of alarming levels of pollutants and greenhouse gases (GHG) in the atmosphere. One of the major...

Recent advances to accelerate purification process development: A review with a focus on vaccines

The safety requirements for vaccines are extremely high since they are administered to healthy people. For that reason, vaccine development is time-consuming and very expensive. Reducing time-to-market is key for pharmaceutical companies, saving lives and money. Therefore the need is raised for systematic, general and efficient process...

High throughput process development for the purification of rapeseed proteins napin and cruciferin by ion exchange chromatography

Proteins derived from plant resources such as oilseed meals, canola and sunflower, are considered a viable alternative to animal proteins for food consumption. This work presents a rational methodology, using high throughput experimentation (HTE), for the separation of cruciferin and napin, the two major proteins of canola meal, by...

Mathematical modelling of expanded bed adsorption - a perspective on in silico process design

Expanded bed adsorption (EBA) emerged in the early 1990s in an attempt to integrate the clarification, capture and initial product concentration/purification process. Several mathematical models have been put forward to describe its operation. However, none of the models developed specifically for EBA allows simultaneous prediction of bed...

Model-based Rational and Systematic Protein Purification Process Development: A Knowledge-based Approach

The increasing market and regulatory (quality and safety) demands on therapeutic proteins calls for radical improvement in their manufacturing processes. Addressing these challenges requires the adoption of strategies and tools that enable faster and more efficient process development. This thesis is concerned with the development and systematic...

Microbial metabolomics: Past, present and future methodologies