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Nedeljković, Marija (author), Mylonas, Akis (author), Wiktor, Virginie (author), Schlangen, E. (author), Visser, Jeanette (author)
Fine recycled concrete aggregates (fRCA, 0–4 mm) are produced from demolished concrete structures and consist of natural aggregates and old cement mortar. The presence of old cement mortar has detrimental effect on the fresh properties and strength of new concrete. This study aims to investigate the working mechanism and effectiveness of...
journal article 2021
document
Palin, D. (author), Mo, Yu (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author)
Presented is a modified test for generating crack permeability data for cementitious materials. Single-parallel cracks were generated in mortar specimens. The width of the cracks was analysed through stereomicroscope and computer tomography, and the water permeability of the cracks was determined. Reduction factors and crack flow models were...
journal article 2019
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Wu, Fabai (author), Japaridze, A. (author), Zheng, X.Z. (author), Wiktor, J.M. (author), Kerssemakers, J.W.J. (author), Dekker, C. (author)
Although the physical properties of chromosomes, including their morphology, mechanics, and dynamics are crucial for their biological function, many basic questions remain unresolved. Here we directly image the circular chromosome in live E. coli with a broadened cell shape. We find that it exhibits a torus topology with, on average, a lower...
journal article 2019
document
Wiktor, J.M. (author), van der Does, M. (author), Büller, L.A. (author), Sherratt, David J. (author), Dekker, C. (author)
The formation of 3 single-stranded DNA overhangs is a first and essential step during homology-directed repair of double-stranded breaks (DSB) of DNA, a task that in Escherichia coli is performed by RecBCD. While this protein complex has been well characterized through in vitro single-molecule studies, it has remained elusive how end...
journal article 2018
document
Sebastian, Antonia (author), Lendering, K.T. (author), van Loon-Steensma, J.M. (author), Paprotny, D. (author), Bellamy, Rob (author), Willems, Patrick (author), van Loenhout, Joris (author), Colaço, Conceição (author), Dias, Susana (author), Nunes, Leónia (author), Rego, Francisco (author), Koundouri, Phoebe (author), Xepapadeas, Petros (author), Vassilopoulos, Achilleas (author), Wiktor, Paweł (author), Wysocka-Golec, Justyna (author)
This report focuses on the methodological development of the testing and implementation framework (TIF) for increasing the socio-technical readiness of climate adaptation innovations and assessing their impact on different socio-economic and environmental sectors. It is designed to be read by innovators and used as a supporting document for the...
report 2017
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Wiktor, J.M. (author)
It seems that the evolution of life on this planet repeatedly acknowledges the value of<br/>the genetic information, by providing an abundant variety of elegant mechanisms for the repair of damage occurring to DNA. The need for such mechanisms tells us that the state of cellular DNA is under constant threat of disintegration and decay. This may...
doctoral thesis 2017
document
Tziviloglou, E. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)
Biogenic self-healing cementitious materials target on the closure of micro-cracks with precipitated inorganic minerals originating from bacterial metabolic activity. Dormant bacterial spores and organic mineral compounds often constitute a biogenic healing agent. The current paper focuses on the investigation of the most appropriate organic...
journal article 2017
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Sebastian, Antonia (author), Lendering, K.T. (author), van Loon-Steensma, J.M. (author), Paprotny, D. (author), Bellamy, Rob (author), Willems, Patrick (author), van Loenhout, Joris (author), Colaço, Conceição (author), Dias, Susana (author), Nunes, Leónia (author), Rego, Francisco (author), Koundouri, Phoebe (author), Xepapadeas, Petros (author), Vassilopoulos, Achilleas (author), Wiktor, Paweł (author), Wysocka-Golec, Justyna (author)
Currently there is no internationally accepted framework for assessing the readiness of innovations that reduce disaster risk. To fill this gap, BRIGAID is developing a standard, comprehensive Testing and Implementation Framework (TIF). The TIF is designed to provide innovators with a framework for innovation and guidelines for assessing an...
report 2017
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Sangadji, S. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)
Bacteria induced calcium carbonate precipitation based on metabolic conversion of nutrients has been acknowledged for having potentials in self-healing cement-based materials. Recent studies have shown the development of bacteria-based repair solution (liquid) for concrete surface repair. This article demonstrates the feasible application of...
conference paper 2017
document
Wiktor, J.M. (author), Lesterlin, Christian (author), Sherratt, David J. (author), Dekker, C. (author)
Programmable control of the cell cycle has been shown to be a powerful tool in cell-biology studies. Here, we develop a novel system for controlling the bacterial cell cycle, based on binding of CRISPR/dCas9 to the origin-of-replication locus. Initiation of replication of bacterial chromosomes is accurately regulated by the DnaA protein,...
journal article 2016
document
Tziviloglou, E. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)
The innovative technology of self-healing concrete allows the material to repair the open micro-cracks that can endanger the structure’s durability, due to ingress of aggressive liquids. Various concepts of self-healing concrete use encapsulation techniques, in order to immobilize and protect the healing agent during mixing and setting. In this...
conference paper 2015
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Wang, J. (author), Mignon, A. (author), Snoeck, D. (author), Wiktor, V.A.C. (author), Van Vliergerghe, S. (author), Boon, N. (author), De Belle, N. (author)
journal article 2015
document
Palin, D. (author), Thijssen, A. (author), Wiktor, V. (author), Jonkers, H.M. (author), Schlangen, H.E.J.G. (author)
Ordinary Portland cement (OPC) mortar specimens submerged in sea-water were analysed through environmental scanning electron microscopy (ESEM) in back scattered electron (BSE) mode and nano-scratching. Results from both sets of analysis show the presence of distinct phases associated with aragonite, brucite and cement paste. Phases associated...
conference paper 2015
document
Wiktor, V. (author), Jonkers, H.M. (author)
Biodeposition, a method by which calcium carbonate (CaCO3) precipitation is induced by bacteria, has been proposed as an interesting approach to protect building materials. The liquid-based system presented in this paper aims at the sealing of cracks and decrease of the porosity due to the production of a calcium-based biomineral. In this system...
conference paper 2015
document
Wiktor, V.A.C. (author), Jonkers, H.M. (author)
This paper presents the field performance in a parking garage of the recently developed bacteria-based repair system for concrete. This liquid-based repair system aims at the sealing of cracks and decrease of the porosity due to the production of a calcium-based biomineral. The system combines advantages of both a traditional repair system for...
journal article 2015
document
Wiktor, V.A.C. (author), Jonkers, H.M. (author)
This paper focuses particularly on the ageing of concrete due to micro-crack formation or freeze/thaw which results in an increased permeability of the concrete. The bacteria-based repair system presented in this paper aims at recovering the concrete permeability thanks to bacteria-induced calcium carbonate precipitation inside cracks/porosity....
conference paper 2014
document
Wiktor, V. (author), Jonkers, H.M. (author)
The goal of this study is to develop a repair system for cementitious materials which features improved properties compared current available systems in terms of long term efficiency and environment friendliness. Thus, a bacteria-based repair system has been developed. The mineral formation mediated by bacterial activity results in the crack...
journal article 2013
document
Sangadji, S. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, H.E.J.G. (author)
Bacteria induced calcite precipitation has been proven to be effective in making concrete structure self-healing. In Microlab TU Delft, the concept has been enhanced by developing a liquid bacteria-based concrete repair system. The solution contains calcite precipitating bacteria, nutrients and buffer compound which may demonstrate high...
conference paper 2013
document
Wiktor, V.A.C. (author), Sangadji, S. (author), Jonkers, H.M. (author), Schlangen, H.E.J.G. (author)
Bacterially induced calcium carbonate precipitation has received considerable attention for its potential application in enforcing or repairing construction material. The mechanism of bacterially mediated calcite precipitation in those studies is primarily based on the enzymatic hydrolysis of urea. Besides calcite precipitation, this reaction...
conference paper 2013
document
Palin, D. (author), Wiktor, V. (author), Jonkers, H.M. (author)
Marine concrete structures are exposed to one of the most hostile of natural environments. Many physical and chemical phenomena are usually interdependent and mutually reinforcing in the deterioration of marine exposed concrete: expansion and microcracking due to physical effects increases concrete permeability paving the way for harmful...
conference paper 2013
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