Whole-Cell Photoenzymatic Cascades to Synthesize Long-Chain Aliphatic Amines and Esters from Renewable Fatty Acids

Long-chain aliphatic amines such as (S,Z)-heptadec-9-en-7-amine and 9-aminoheptadecane were synthesized from ricinoleic acid and oleic acid, respectively, by whole-cell cascade reactions using the combination of an alcohol dehydrogenase (ADH) from Micrococcus luteus, an engineered amine transaminase from Vibrio fluvialis (Vf-ATA), and a...

How To Break the Janus Effect of H ₂ O ₂ in Biocatalysis? Understanding Inactivation Mechanisms To Generate more Robust Enzymes

H ₂ O ₂ , is an attractive oxidant for synthetic chemistry, especially if activated as percarboxylic acid. H ₂ O ₂ , however, is also a potent inactivator of enzymes. Protein engineering efforts to improve enzyme resistance against H ₂ O ₂ in the past have mostly focused on...