Zhang, Linyue (author), King, Edward (author), Black, William B. (author), Heckmann, C.M. (author), Wolder, A.E. (author), Cui, Youtian (author), Nicklen, Francis (author), Siegel, Justin B. (author), Paul, C.E. (author) Noncanonical redox cofactors are attractive low-cost alternatives to nicotinamide adenine dinucleotide (phosphate) (NAD(P)<sup>+</sup>) in biotransformation. However, engineering enzymes to utilize them is challenging. Here, we present a high-throughput directed evolution platform which couples cell growth to the in vivo cycling of a...
journal article 2022
