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Moolman, M.C. (author), Tiruvadi Krishnan, S. (author), Kerssemakers, J.W.J. (author), de Leeuw, R. (author), Lorent, V.J.F. (author), Sherratt, David J. (author), Dekker, N.H. (author)Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in the cell are poorly understood....journal article 2016
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Krishnan, S.T. (author), Moolman, M.C. (author), Van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)Background Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date, ?...journal article 2015
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Tiruvadi Krishnan, S. (author), Moolman, M.C. (author), van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)Background: Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date,...journal article 2015
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Moolman, M.C. (author), Tiruvadi Krishnan, S. (author), Kerssemakers, J.W.J. (author), Van den Berg, A. (author), Tulinski, P. (author), Depken, M. (author), Reyes-Lamothe, R. (author), Sherratt, D.J. (author), Dekker, N.H. (author)The ubiquitous sliding clamp facilitates processivity of the replicative polymerase and acts as a platform to recruit proteins involved in replication, recombination and repair. While the dynamics of the E. coli ?2-sliding clamp have been characterized in vitro, its in vivo stoichiometry and dynamics remain unclear. To probe both ?2-clamp...journal article 2014
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Moolman, M.C. (author), Huang, Z. (author), Krishnan, S.T. (author), Kerssemakers, J.W.J. (author), Dekker, N.H. (author)Background: Controlled restriction of cellular movement using microfluidics allows one to study individual cells to gain insight into aspects of their physiology and behaviour. For example, the use of micron-sized growth channels that confine individual Escherichia coli has yielded novel insights into cell growth and death. To extend this...journal article 2013
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Dulin, D. (author), Lipfert, J. (author), Moolman, M.C. (author), Dekker, N.H. (author)To understand genomic processes such as transcription, translation or splicing, we need to be able to study their spatial and temporal organization at the molecular level. Single-molecule approaches provide this opportunity, allowing researchers to monitor molecular conformations, interactions or diffusion quantitatively and in real time in...journal article 2013