Pontiella desulfatans gen. Nov., sp. nov., and pontiella sulfatireligans sp. nov., two marine anaerobes of the pontiellaceae fam. nov. producing sulfated glycosaminoglycan-like exopolymers

Recently, we isolated two marine strains, F1^T and F21^T, which together with Kiritimatiella glycovorans L21-Fru-AB^T are the only pure cultures of the class Kiritimatiellae within the phylum Verrucomicrobiota. Here, we present an in-depth genome-guided characterization of both isolates with emphasis on their...

Aerobic granular sludge contains Hyaluronic acid-like and sulfated glycosaminoglycans-like polymers

Glycosaminoglycans (GAGs) are linear heteropolysaccharides containing a derivative of an amino sugar. The possibility of the presence of GAGs in aerobic granular sludge was studied by combining SDS-PAGE with Alcian Blue staining (at pH 2.5 and 1), FTIR, mammalian Hyaluronic acid and sulfated GAG analysis kits, enzymatic digestion and specific...