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Perez, D.I. (author), Grau, M.M. (author), Arends, I.W.C.E. (author), Hollmann, F. (author)Robust peroxidase-catalyzed enantiospecific oxyfunctionalizations can be achieved by simple light-driven in situ generation of hydrogen peroxide.journal article 2009
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Lloret, L. (author), Hollmann, F. (author), Eibes, G. (author), Feijoo, G. (author), Moreira, M.T. (author), Lema, J.M. (author)Laccase from Myceliophthora thermophila was covalently immobilised on Eupergit C and Eupergit C 250L yielding specific activities of up to 17 and 80 U/g, respectively. Due to its superior activity, Eupergit C 250L was chosen for further research. The somewhat lower catalytic efficiency (based on the ratio between the turnover number and the...journal article 2011
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Kochius, S. (author), Magnusson, A.O. (author), Hollmann, F. (author), Schrader, J. (author), Holtmann, D. (author)The applicability of dissolved redox mediators for NAD(P)+ regeneration has been demonstrated several times. Nevertheless, the use of mediators in solutions for sensor applications is not a very convenient strategy since the analysis is not reagentless and long stabilization times occur. The most important drawbacks of dissolved mediators in...journal article 2012
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Hollmann, F. (author), Arends, I.W.C.E. (author)Biocatalysis is propagating into practically every area of organic chemistry, amongst them radical polymerizations. A review of the recent developments of this dynamic and quickly evolving area of research is presented together with a critical evaluation of its potential to yield novel polymers and/or environmentally more benign synthetic...journal article 2012
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De Torres, M. (author), Dimroth, J. (author), Arends, I.W.C.E. (author), Keilitz, J. (author), Hollmann, F. (author)Rh(III)-TsDPEN, an immobilized analog of the well-known [Cp*Rh(bpy)(H2O)]2+ was evaluated as a heterogeneous, recyclable regeneration catalyst for reduced oxidoreductase cofactors [NAD(P)H]. Repeated use of this catalyst was established and the catalytic properties were initially investigated. Apparently, Rh(III)-TsDPEN is prone to severe...journal article 2012
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Kara, S. (author), Spickermann, D. (author), Schrittwieser, J.H. (author), Leggewie, C. (author), Van Berkel, W.J.H. (author), Arendsa, I.W.C.E. (author), Hollmann, F. (author)1,4-Butanediol is shown to be an efficient cosubstrate to promote NAD(P)H-dependent redox biocatalysis. The thermodynamically and kinetically inert lactone coproduct makes the regeneration reaction irreversible. Thereby not only the molar surplus of cosubstrate is dramatically reduced but also faster reaction rates are obtained.journal article 2012
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- Perez de los Rios, A. (author), Irabien, A. (author), Hollmann, F. (author), Fernandez, J.H. (author) journal article 2013
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Schrittwieser, J. (author), Coccia, F. (author), Kara, S. (author), Grischek, B. (author), Kroutil, W. (author), d'Alessandro, N. (author), Hollmann, F. (author)One-pot combinations of sequential catalytic reactions can offer practical and ecological advantages over classical multi-step synthesis schemes. In this context, the integration of enzymatic and chemo-catalytic transformations holds particular potential for efficient and selective reaction sequences that would not be 10 possible using either...journal article 2013
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Holtmann, D. (author), Fraaije, M.W. (author), Arends, I.W.C.E. (author), Oppermand, D.J. (author), Hollmann, F. (author)The scope and limitations of oxygenases as catalysts for preparative organic synthesis is discussed.journal article 2014
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- Bornadel, A (author), Hatti-Kaul, R (author), Hollmann, F. (author), Kara, S (author) journal article 2015
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Bormann, S. (author), Gomez Baraibar, A. (author), Ni, Y. (author), Holtmann, D. (author), Hollmann, F. (author)Peroxygenases enable H2O2-driven oxyfunctionalisation of organic compounds such as selective epoxidation, hydroxylation and heteroatom oxygenation. Therefore, peroxygenases are potentially very useful tools for the organic chemist. This contribution reviews the current state of the art in peroxygenases, their availability, engineering and...journal article 2015
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Dominguez de Maria, P (author), Hollmann, F. (author)Biocatalysis is generally regarded as a “green” technology. This statement is justified by the mild reaction conditions, the use of aqueous reaction media—with water as the paradigm of green solvents—, and the renewable nature of the biocatalysts. However, researchers making these statements frequently do not take into account the entire picture...journal article 2015
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Knaus, T (author), Paul, C.E. (author), Levy, CW (author), de Vries, S. (author), Mutti, FG (author), Hollmann, F. (author), Scrutton, NS (author)The search for affordable, green biocatalytic processes is a challenge for chemicals manufacture. Redox biotransformations are potentially attractive, but they rely on<br/>unstable and expensive nicotinamide coenzymes that have prevented their widespread exploitation. Stoichiometric use of natural coenzymes is not viable economically, and the...journal article 2016
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Ni, Y. (author), Hollmann, F. (author)Oxidoreductases are promising catalysts for organic synthesis. To sustain their catalytic cycles they require efficient supply with redox equivalents. Today classical biomimetic approaches utilizing natural electron supply chains prevail but artificial regeneration approaches bear the promise of simpler and more robust reaction schemes....book chapter 2016
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Rauch, M.C.R. (author), Schmidt, S. (author), Arends, I.W.C.E. (author), oppelt, K. (author), Kara, S (author), Hollmann, F. (author)The photocatalytic oxidation of NADH using a flavin photocatalyst and a simple blue LED light source is reported. This in situ NAD+ regeneration system can be used to promote biocatalytic, enantioselective oxidation reactions. Compared to the traditional use of white light bulbs this method enables very significant reductions in energy...journal article 2016
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Fernandez Fueyo, E. (author), Younes, S.H.H. (author), Van Rootselaar, Stefan (author), Aben, René W M (author), Renirie, R. (author), Wever, Ron (author), Holtmann, Dirk (author), Rutjes, Floris P J T (author), Hollmann, F. (author)A catalytic, enzyme-initiated (aza-) Achmatowicz reaction is presented. The involvement of a robust vanadium-dependent peroxidase from Curvularia inaequalis allows the simple use of H<sub>2</sub>O<sub>2</sub> and catalytic amounts of bromide.journal article 2016
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Bissaro, Bastien (author), Forsberg, Zarah (author), Ni, Y. (author), Hollmann, F. (author), Vaaje-Kolstad, Gustav (author), Eijsink, Vincent G H (author)Photosynthesis may be described as light-driven oxidation of water and subsequent use of the generated reducing equivalents to fix CO<sub>2</sub> and synthesize higher energy organic compounds, such as carbohydrates. The transposition of the sustainable and atom-efficient strategy of water oxidation to in vitro controlled biocatalytic...journal article 2016
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Fernandez Fueyo, E. (author), Ni, Y. (author), Gomez Baraibar, A. (author), Alcalde, Miguel (author), van Langen, L.M. (author), Hollmann, F. (author)The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and <sup>tert</sup...journal article 2016
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Peroxygenase-catalyzed oxyfunctionalization reactions promoted by the complete oxidation of methanolNi, Y. (author), Fernandez Fueyo, E. (author), Baraibar, AG (author), Ullrich, R (author), Hofrichter, Martin (author), Yanase, H (author), Alcalde, Miguel (author), van Berkel, WJH (author), Hollmann, F. (author)Peroxygenases catalyze a broad range of (stereo)selective oxyfunctionalization reactions. However, to access their full catalytic potential, peroxygenases need a balanced provision of hydrogen peroxide to achieve high catalytic activity while minimizing oxidative inactivation. Herein, we report an enzymatic cascade process that employs methanol...journal article 2016
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Paul, C.E. (author), Hollmann, F. (author)Synthetic nicotinamide cofactors are analogues of the natural cofactors used by oxidoreductases as redox intermediates. Their ability to be fine-tuned makes these biomimetics an attractive alternative to the natural cofactors in terms of stability, reactivity, and cost. The following mini-review focuses on the current state of the art of...journal article 2016