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- Holtmann, Dirk (author), Hollmann, F. (author) journal article 2022
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Bouchaut, B.F.H.J. (author), Hollmann, F. (author), Asveld, L. (author)The increasing societal demand for safer, biobased products, and processes<br/>creates opportunities for industrial biotechnology and chemistry. To succeed,<br/>controlled learning about new emerging risks is crucial but both fields endure<br/>difficulty in doing so by their respective regulation and risk management culture.journal article 2022
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Puchľová, Eva (author), Hilberath, T. (author), Vranková, Kvetoslava (author), Hollmann, F. (author)Non-enantioselective alcohol dehydrogenases (ADHs) are rarely found in the biocatalysis portfolio. Generally, highly enantioselective ADHs are sought for. Using such ADHs for the oxidation of racemic alcohols generally results in a kinetic resolution of the starting material, which is unfavourable if the ketone represents the product of interest...journal article 2022
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França, Alexandre S. (author), Brêda, Gabriela C. (author), De Oliveira, Kleber T. (author), Almeida, Rodrigo V. (author), Hollmann, F. (author), de Souza, Rodrigo O.M.A. (author)A visible-light-driven photocatalytic decarboxylation of palmitic acid and related fatty acids is described in this study. Remarkable decarboxylation rates have been observed with full conversion in less than 20 min. In this study, we have<br/>demonstrated that sunlight irradiation, even on cloudy days, can deliver similar results to traditional...journal article 2023
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Knaus, T (author), Paul, C.E. (author), Levy, CW (author), de Vries, S. (author), Mutti, FG (author), Hollmann, F. (author), Scrutton, NS (author)The search for affordable, green biocatalytic processes is a challenge for chemicals manufacture. Redox biotransformations are potentially attractive, but they rely on<br/>unstable and expensive nicotinamide coenzymes that have prevented their widespread exploitation. Stoichiometric use of natural coenzymes is not viable economically, and the...journal article 2016
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- Bornadel, A (author), Hatti-Kaul, R (author), Hollmann, F. (author), Kara, S (author) journal article 2015
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Gjonaj, L. (author), Pinkse, M.W.H. (author), Fernandez Fueyo, E. (author), Hollmann, F. (author), Hanefeld, U. (author)Nitrile reductases catalyse a two-step reduction of nitriles to amines. This requires the binding of two NADPH molecules during one catalytic cycle. For the nitrile reductase from E. coli (EcoNR) mass spectrometry studies of the catalytic mechanism were performed. EcoNR is dimeric and has no Rossman fold. It was demonstrated that during...journal article 2016
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Dong, J. (author), Fernandez Fueyo, E. (author), Li, Jingbo (author), Guo, Zheng (author), Renirie, Rokus (author), Wever, Ron (author), Hollmann, F. (author)The vanadium-dependent chloroperoxidase from Curvularia inaequalis is a stable and efficient biocatalyst for the hydroxyhalogenation of a broad range of alkenes into halohydrins. Up to 1 200 000 TON with 69 s−1 TOF were observed for the biocatalyst. A bienzymatic cascade to yield epoxides as reaction products is presented.journal article 2017
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Zhou, Pengfei (author), Wang, Xuping (author), Yang, Bo (author), Hollmann, F. (author), Wang, Yonghua (author)Epoxides are important synthetic intermediates for the synthesis of a broad range of industrial products. This study presents a promising solution to the current limitation of enzyme instability. By using simple deep eutectic solvents such as choline chloride/sorbitol, significant stabilization of the biocatalyst has been achieved leading to...journal article 2017
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Kim, Jinhyun (author), Lee, S.H. (author), Tieves, F. (author), Choi, Da Som (author), Hollmann, F. (author), Paul, C.E. (author), Park, Chan Beum (author)Light-driven activation of redox enzymes is an emerging route for sustainable chemical synthesis. Among redox enzymes, the family of Old Yellow Enzyme (OYE) dependent on the nicotinamide adenine dinucleotide cofactor (NADH) catalyzes the stereoselective reduction of α,β-unsaturated hydrocarbons. Here, we report OYE-catalyzed asymmetric...journal article 2018
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Biocatalytic Aromaticity-Breaking Epoxidation of Naphthalene and Nucleophilic Ring-Opening ReactionsZhang, W. (author), Li, Huanhuan (author), Younes, S.H.H. (author), Gómez De Santos, Patricia (author), Tieves, F. (author), Grogan, Gideon (author), Pabst, Martin (author), Alcalde, Miguel (author), Whitwood, Adrian C. (author), Hollmann, F. (author)Aromatic hydroxylation reactions catalyzed by heme-thiolate enzymes proceed via an epoxide intermediate. These aromatic epoxides could be valuable building blocks for organic synthesis giving access to a range of chiral trans-disubstituted cyclohexadiene synthons. Here, we show that naphthalene epoxides generated by fungal peroxygenases can...journal article 2021
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Al-Shameri, Ammar (author), Willot, S.J. (author), Paul, C.E. (author), Hollmann, F. (author), Lauterbach, Lars (author)Correction for 'H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions' by Ammar Al-Shameri et al., Chem. Commun., 2020, DOI: 10.1039/d0cc03229h.journal article 2020
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Duong, Hong T. (author), Wu, Y. (author), Sutor, Alexander (author), Burek, Bastien O. (author), Hollmann, F. (author), Bloh, Jonathan Z. (author)Light-driven biocatalytic processes are notoriously hampered by poor penetration of light into the turbid reaction media. In this study, wirelessly powered light-emitting diodes are found to represent an efficient and scalable approach for process intensification of the photobiosynthetic production of diesel alkanes from renewable fatty acids...journal article 2021
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Dirks, Tim (author), Yayci, Abdulkadir (author), Klopsch, Sabrina (author), Krewing, Marco (author), Zhang, Wuyuan (author), Hollmann, F. (author), Bandow, Julia E. (author)Non-thermal plasmas are used in various applications to inactivate biological agents or biomolecules. A complex cocktail of reactive species, (vacuum) UV radiation and in some cases exposure to an electric field together cause the detrimental effects. In contrast to this disruptive property of technical plasmas, we have shown previously that...journal article 2023
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Aslan-Üzel, Aşkın S. (author), Beier, Andy (author), Kovář, David (author), Cziegler, Clemens (author), Padhi, Santosh K. (author), Schuiten, Eva D. (author), Dörr, Mark (author), Böttcher, Dominique (author), Hollmann, F. (author)Halide assays are important for the study of enzymatic dehalogenation, a topic of great industrial and scientific importance. Here we describe the development of a very sensitive halide assay that can detect less than a picomole of bromide ions, making it very useful for quantifying enzymatic dehalogenation products. Halides are oxidised...journal article 2019
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Reeve, Holly A. (author), Nicholson, Jake (author), Altaf, Farieha (author), Lonsdale, Thomas H. (author), Preissler, Janina (author), Lauterbach, Lars (author), Lenz, Oliver (author), Leimkühler, Silke (author), Hollmann, F. (author), Paul, C.E. (author), Vincent, Kylie A. (author)We demonstrate a recycling system for synthetic nicotinamide cofactor analogues using a soluble hydrogenase with turnover number of >1000 for reduction of the cofactor analogues by H2. Coupling this system to an ene reductase, we show quantitative conversion of N-ethylmaleimide to N-ethylsuccinimide. The biocatalyst system retained >50%...journal article 2022
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Hobisch, Markus (author), van Schie, M.M.C.H. (author), Kim, Jinhyun (author), Røjkjær Andersen, Kasper (author), Alcalde, Miguel (author), Kourist, Robert (author), Park, Chan Beum (author), Hollmann, F. (author), Kara, S. (author)The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H<sub>2</sub>O<sub>2</sub> was photocatalytically generated in...journal article 2020
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Al-Shameri, Ammar (author), Willot, S.J. (author), Paul, C.E. (author), Hollmann, F. (author), Lauterbach, Lars (author)The soluble hydrogenase from Ralstonia eutropha provides an atom efficient regeneration system for reduced flavin cofactors using H2 as an electron source. We demonstrated this system for highly selective ene-reductase-catalyzed CC-double bond reductions and monooxygenase-catalyzed epoxidation. Reactions were expanded to aerobic conditions to...journal article 2020
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Evolved Peroxygenase-Aryl Alcohol Oxidase Fusions for Self-Sufficient Oxyfunctionalization ReactionsGomez De Santos, Patricia (author), Lazaro, Sofia (author), Viña-Gonzalez, Javier (author), Hoang, Manh Dat (author), Sánchez-Moreno, Israel (author), Glieder, Anton (author), Hollmann, F. (author), Alcalde, Miguel (author)Fungal peroxygenases are deemed emergent biocatalysts for selective C-H bond oxyfunctionalization reactions. In this study, we have engineered a functional and stable self-sufficient chimeric peroxygenase-oxidase fusion. The bifunctional biocatalyst carried a laboratory-evolved version of the fungal peroxygenase fused to an evolved fungal...journal article 2020
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Yayci, Abdulkadir (author), Dirks, Tim (author), Kogelheide, Friederike (author), Alcalde, Miguel (author), Hollmann, F. (author), Awakowicz, Peter (author), Bandow, Julia E. (author)In plasma-driven biocatalysis, enzymes are employed to carry out reactions using species generated by non-thermal plasmas as the precursors. We have previously demonstrated that this is feasible in principle, but that the approach suffers from the short lifetime of the biocatalyst under operating conditions. In this work, protection...journal article 2020